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Yeast U6 snRNA made by RNA polymerase II is less stable but functional

U6 small nuclear (sn)RNA is the shortest and most conserved snRNA in the spliceosome and forms a substantial portion of its active site. Unlike the other four spliceosomal snRNAs, which are synthesized by RNA polymerase (RNAP) II, U6 is made by RNAP III. To determine if some aspect of U6 function is...

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Autores principales: Lipinski, Karli A., Chi, Jing, Chen, Xin, Hoskins, Aaron A., Brow, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9670810/
https://www.ncbi.nlm.nih.gov/pubmed/36195346
http://dx.doi.org/10.1261/rna.079328.122
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author Lipinski, Karli A.
Chi, Jing
Chen, Xin
Hoskins, Aaron A.
Brow, David A.
author_facet Lipinski, Karli A.
Chi, Jing
Chen, Xin
Hoskins, Aaron A.
Brow, David A.
author_sort Lipinski, Karli A.
collection PubMed
description U6 small nuclear (sn)RNA is the shortest and most conserved snRNA in the spliceosome and forms a substantial portion of its active site. Unlike the other four spliceosomal snRNAs, which are synthesized by RNA polymerase (RNAP) II, U6 is made by RNAP III. To determine if some aspect of U6 function is incompatible with synthesis by RNAP II, we created a U6 snRNA gene with RNAP II promoter and terminator sequences. This “U6-II” gene is functional as the sole source of U6 snRNA in yeast, but its transcript is much less stable than U6 snRNA made by RNAP III. Addition of the U4 snRNA Sm protein binding site to U6-II increased its stability and led to formation of U6-II•Sm complexes. We conclude that synthesis of U6 snRNA by RNAP III is not required for its function and that U6 snRNPs containing the Sm complex can form in vivo. The ability to synthesize U6 snRNA with RNAP II relaxes sequence restraints imposed by intragenic RNAP III promoter and terminator elements and allows facile control of U6 levels via regulators of RNAP II transcription.
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spelling pubmed-96708102023-12-01 Yeast U6 snRNA made by RNA polymerase II is less stable but functional Lipinski, Karli A. Chi, Jing Chen, Xin Hoskins, Aaron A. Brow, David A. RNA Article U6 small nuclear (sn)RNA is the shortest and most conserved snRNA in the spliceosome and forms a substantial portion of its active site. Unlike the other four spliceosomal snRNAs, which are synthesized by RNA polymerase (RNAP) II, U6 is made by RNAP III. To determine if some aspect of U6 function is incompatible with synthesis by RNAP II, we created a U6 snRNA gene with RNAP II promoter and terminator sequences. This “U6-II” gene is functional as the sole source of U6 snRNA in yeast, but its transcript is much less stable than U6 snRNA made by RNAP III. Addition of the U4 snRNA Sm protein binding site to U6-II increased its stability and led to formation of U6-II•Sm complexes. We conclude that synthesis of U6 snRNA by RNAP III is not required for its function and that U6 snRNPs containing the Sm complex can form in vivo. The ability to synthesize U6 snRNA with RNAP II relaxes sequence restraints imposed by intragenic RNAP III promoter and terminator elements and allows facile control of U6 levels via regulators of RNAP II transcription. Cold Spring Harbor Laboratory Press 2022-12 /pmc/articles/PMC9670810/ /pubmed/36195346 http://dx.doi.org/10.1261/rna.079328.122 Text en © 2022 Lipinski et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society https://creativecommons.org/licenses/by-nc/4.0/This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Article
Lipinski, Karli A.
Chi, Jing
Chen, Xin
Hoskins, Aaron A.
Brow, David A.
Yeast U6 snRNA made by RNA polymerase II is less stable but functional
title Yeast U6 snRNA made by RNA polymerase II is less stable but functional
title_full Yeast U6 snRNA made by RNA polymerase II is less stable but functional
title_fullStr Yeast U6 snRNA made by RNA polymerase II is less stable but functional
title_full_unstemmed Yeast U6 snRNA made by RNA polymerase II is less stable but functional
title_short Yeast U6 snRNA made by RNA polymerase II is less stable but functional
title_sort yeast u6 snrna made by rna polymerase ii is less stable but functional
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9670810/
https://www.ncbi.nlm.nih.gov/pubmed/36195346
http://dx.doi.org/10.1261/rna.079328.122
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