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Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
[Image: see text] The repulsive electrostatic force between a biomolecule and a like-charged surface can be geometrically tailored to create spatial traps for charged molecules in solution. Using a parallel-plate system composed of silicon dioxide surfaces, we recently demonstrated single-molecule t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9671048/ https://www.ncbi.nlm.nih.gov/pubmed/36326814 http://dx.doi.org/10.1021/acs.langmuir.2c02203 |
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author | Bespalova, Maria Öz, Robin Westerlund, Fredrik Krishnan, Madhavi |
author_facet | Bespalova, Maria Öz, Robin Westerlund, Fredrik Krishnan, Madhavi |
author_sort | Bespalova, Maria |
collection | PubMed |
description | [Image: see text] The repulsive electrostatic force between a biomolecule and a like-charged surface can be geometrically tailored to create spatial traps for charged molecules in solution. Using a parallel-plate system composed of silicon dioxide surfaces, we recently demonstrated single-molecule trapping and high precision molecular charge measurements in a nanostructured free energy landscape. Here we show that surfaces coated with charged lipid bilayers provide a system with tunable surface properties for molecular electrometry experiments. Working with molecular species whose effective charge and geometry are well-defined, we demonstrate the ability to quantitatively probe the electrical charge density of a supported lipid bilayer. Our findings indicate that the fraction of charged lipids in nanoslit lipid bilayers can be significantly different from that in the precursor lipid mixtures used to generate them. We also explore the temporal stability of bilayer properties in nanofluidic systems. Beyond their relevance in molecular measurement, such experimental systems offer the opportunity to examine lipid bilayer formation and wetting dynamics on nanostructured surfaces. |
format | Online Article Text |
id | pubmed-9671048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-96710482022-11-18 Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System Bespalova, Maria Öz, Robin Westerlund, Fredrik Krishnan, Madhavi Langmuir [Image: see text] The repulsive electrostatic force between a biomolecule and a like-charged surface can be geometrically tailored to create spatial traps for charged molecules in solution. Using a parallel-plate system composed of silicon dioxide surfaces, we recently demonstrated single-molecule trapping and high precision molecular charge measurements in a nanostructured free energy landscape. Here we show that surfaces coated with charged lipid bilayers provide a system with tunable surface properties for molecular electrometry experiments. Working with molecular species whose effective charge and geometry are well-defined, we demonstrate the ability to quantitatively probe the electrical charge density of a supported lipid bilayer. Our findings indicate that the fraction of charged lipids in nanoslit lipid bilayers can be significantly different from that in the precursor lipid mixtures used to generate them. We also explore the temporal stability of bilayer properties in nanofluidic systems. Beyond their relevance in molecular measurement, such experimental systems offer the opportunity to examine lipid bilayer formation and wetting dynamics on nanostructured surfaces. American Chemical Society 2022-11-03 2022-11-15 /pmc/articles/PMC9671048/ /pubmed/36326814 http://dx.doi.org/10.1021/acs.langmuir.2c02203 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Bespalova, Maria Öz, Robin Westerlund, Fredrik Krishnan, Madhavi Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System |
title | Single-Molecule
Trapping and Measurement in a Nanostructured
Lipid Bilayer System |
title_full | Single-Molecule
Trapping and Measurement in a Nanostructured
Lipid Bilayer System |
title_fullStr | Single-Molecule
Trapping and Measurement in a Nanostructured
Lipid Bilayer System |
title_full_unstemmed | Single-Molecule
Trapping and Measurement in a Nanostructured
Lipid Bilayer System |
title_short | Single-Molecule
Trapping and Measurement in a Nanostructured
Lipid Bilayer System |
title_sort | single-molecule
trapping and measurement in a nanostructured
lipid bilayer system |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9671048/ https://www.ncbi.nlm.nih.gov/pubmed/36326814 http://dx.doi.org/10.1021/acs.langmuir.2c02203 |
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