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Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System

[Image: see text] The repulsive electrostatic force between a biomolecule and a like-charged surface can be geometrically tailored to create spatial traps for charged molecules in solution. Using a parallel-plate system composed of silicon dioxide surfaces, we recently demonstrated single-molecule t...

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Autores principales: Bespalova, Maria, Öz, Robin, Westerlund, Fredrik, Krishnan, Madhavi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9671048/
https://www.ncbi.nlm.nih.gov/pubmed/36326814
http://dx.doi.org/10.1021/acs.langmuir.2c02203
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author Bespalova, Maria
Öz, Robin
Westerlund, Fredrik
Krishnan, Madhavi
author_facet Bespalova, Maria
Öz, Robin
Westerlund, Fredrik
Krishnan, Madhavi
author_sort Bespalova, Maria
collection PubMed
description [Image: see text] The repulsive electrostatic force between a biomolecule and a like-charged surface can be geometrically tailored to create spatial traps for charged molecules in solution. Using a parallel-plate system composed of silicon dioxide surfaces, we recently demonstrated single-molecule trapping and high precision molecular charge measurements in a nanostructured free energy landscape. Here we show that surfaces coated with charged lipid bilayers provide a system with tunable surface properties for molecular electrometry experiments. Working with molecular species whose effective charge and geometry are well-defined, we demonstrate the ability to quantitatively probe the electrical charge density of a supported lipid bilayer. Our findings indicate that the fraction of charged lipids in nanoslit lipid bilayers can be significantly different from that in the precursor lipid mixtures used to generate them. We also explore the temporal stability of bilayer properties in nanofluidic systems. Beyond their relevance in molecular measurement, such experimental systems offer the opportunity to examine lipid bilayer formation and wetting dynamics on nanostructured surfaces.
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spelling pubmed-96710482022-11-18 Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System Bespalova, Maria Öz, Robin Westerlund, Fredrik Krishnan, Madhavi Langmuir [Image: see text] The repulsive electrostatic force between a biomolecule and a like-charged surface can be geometrically tailored to create spatial traps for charged molecules in solution. Using a parallel-plate system composed of silicon dioxide surfaces, we recently demonstrated single-molecule trapping and high precision molecular charge measurements in a nanostructured free energy landscape. Here we show that surfaces coated with charged lipid bilayers provide a system with tunable surface properties for molecular electrometry experiments. Working with molecular species whose effective charge and geometry are well-defined, we demonstrate the ability to quantitatively probe the electrical charge density of a supported lipid bilayer. Our findings indicate that the fraction of charged lipids in nanoslit lipid bilayers can be significantly different from that in the precursor lipid mixtures used to generate them. We also explore the temporal stability of bilayer properties in nanofluidic systems. Beyond their relevance in molecular measurement, such experimental systems offer the opportunity to examine lipid bilayer formation and wetting dynamics on nanostructured surfaces. American Chemical Society 2022-11-03 2022-11-15 /pmc/articles/PMC9671048/ /pubmed/36326814 http://dx.doi.org/10.1021/acs.langmuir.2c02203 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Bespalova, Maria
Öz, Robin
Westerlund, Fredrik
Krishnan, Madhavi
Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
title Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
title_full Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
title_fullStr Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
title_full_unstemmed Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
title_short Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
title_sort single-molecule trapping and measurement in a nanostructured lipid bilayer system
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9671048/
https://www.ncbi.nlm.nih.gov/pubmed/36326814
http://dx.doi.org/10.1021/acs.langmuir.2c02203
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