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Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion
Normothermic machine perfusion (NMP) is nowadays frequently utilized in liver transplantation. Despite commonly accepted viability assessment criteria, such as perfusate lactate and perfusate pH, there is a lack of predictive organ evaluation strategies to ensure graft viability. Hyperspectral imagi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9671746/ https://www.ncbi.nlm.nih.gov/pubmed/36406899 http://dx.doi.org/10.1097/TXD.0000000000001420 |
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author | Kneifel, Felicia Wagner, Tristan Flammang, Isabelle Vogt, Franziska Katou, Shadi Vogel, Thomas Houben, Philipp Becker, Felix Wahl, Philip Pascher, Andreas Radunz, Sonia |
author_facet | Kneifel, Felicia Wagner, Tristan Flammang, Isabelle Vogt, Franziska Katou, Shadi Vogel, Thomas Houben, Philipp Becker, Felix Wahl, Philip Pascher, Andreas Radunz, Sonia |
author_sort | Kneifel, Felicia |
collection | PubMed |
description | Normothermic machine perfusion (NMP) is nowadays frequently utilized in liver transplantation. Despite commonly accepted viability assessment criteria, such as perfusate lactate and perfusate pH, there is a lack of predictive organ evaluation strategies to ensure graft viability. Hyperspectral imaging (HSI)—as an optical imaging modality increasingly applied in the biomedical field—might provide additional useful data regarding allograft viability and performance of liver grafts during NMP. METHODS. Twenty-five deceased donor liver allografts were included in the study. During NMP, graft viability was assessed conventionally and by means of HSI. Images of liver parenchyma were acquired at 1, 2, and 4 h of NMP, and subsequently analyzed using a specialized HSI acquisition software to compute oxygen saturation, tissue hemoglobin index, near-infrared perfusion index, and tissue water index. To analyze the association between HSI parameters and perfusate lactate as well as perfusate pH, we performed simple linear regression analysis. RESULTS. Perfusate lactate at 1, 2, and 4 h NMP was 1.5 [0.3–8.1], 0.9 [0.3–2.8], and 0.9 [0.1–2.2] mmol/L. Perfusate pH at 1, 2, and 4 h NMP was 7.329 [7.013–7.510], 7.318 [7.081–7.472], and 7.265 [6.967–7.462], respectively. Oxygen saturation predicted perfusate lactate at 1 and 2 h NMP (R(2) = 0.1577, P = 0.0493; R(2) = 0.1831, P = 0.0329; respectively). Tissue hemoglobin index predicted perfusate lactate at 1, 2, and 4 h NMP (R(2) = 0.1916, P = 0.0286; R(2) = 0.2900, P = 0.0055; R(2) = 0.2453, P = 0.0139; respectively). CONCLUSIONS. HSI may serve as a noninvasive tool for viability assessment during NMP. Further evaluation and validation of HSI parameters are warranted in larger sample sizes. |
format | Online Article Text |
id | pubmed-9671746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-96717462022-11-18 Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion Kneifel, Felicia Wagner, Tristan Flammang, Isabelle Vogt, Franziska Katou, Shadi Vogel, Thomas Houben, Philipp Becker, Felix Wahl, Philip Pascher, Andreas Radunz, Sonia Transplant Direct Liver Transplantation Normothermic machine perfusion (NMP) is nowadays frequently utilized in liver transplantation. Despite commonly accepted viability assessment criteria, such as perfusate lactate and perfusate pH, there is a lack of predictive organ evaluation strategies to ensure graft viability. Hyperspectral imaging (HSI)—as an optical imaging modality increasingly applied in the biomedical field—might provide additional useful data regarding allograft viability and performance of liver grafts during NMP. METHODS. Twenty-five deceased donor liver allografts were included in the study. During NMP, graft viability was assessed conventionally and by means of HSI. Images of liver parenchyma were acquired at 1, 2, and 4 h of NMP, and subsequently analyzed using a specialized HSI acquisition software to compute oxygen saturation, tissue hemoglobin index, near-infrared perfusion index, and tissue water index. To analyze the association between HSI parameters and perfusate lactate as well as perfusate pH, we performed simple linear regression analysis. RESULTS. Perfusate lactate at 1, 2, and 4 h NMP was 1.5 [0.3–8.1], 0.9 [0.3–2.8], and 0.9 [0.1–2.2] mmol/L. Perfusate pH at 1, 2, and 4 h NMP was 7.329 [7.013–7.510], 7.318 [7.081–7.472], and 7.265 [6.967–7.462], respectively. Oxygen saturation predicted perfusate lactate at 1 and 2 h NMP (R(2) = 0.1577, P = 0.0493; R(2) = 0.1831, P = 0.0329; respectively). Tissue hemoglobin index predicted perfusate lactate at 1, 2, and 4 h NMP (R(2) = 0.1916, P = 0.0286; R(2) = 0.2900, P = 0.0055; R(2) = 0.2453, P = 0.0139; respectively). CONCLUSIONS. HSI may serve as a noninvasive tool for viability assessment during NMP. Further evaluation and validation of HSI parameters are warranted in larger sample sizes. Lippincott Williams & Wilkins 2022-11-17 /pmc/articles/PMC9671746/ /pubmed/36406899 http://dx.doi.org/10.1097/TXD.0000000000001420 Text en Copyright © 2022 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Liver Transplantation Kneifel, Felicia Wagner, Tristan Flammang, Isabelle Vogt, Franziska Katou, Shadi Vogel, Thomas Houben, Philipp Becker, Felix Wahl, Philip Pascher, Andreas Radunz, Sonia Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion |
title | Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion |
title_full | Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion |
title_fullStr | Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion |
title_full_unstemmed | Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion |
title_short | Hyperspectral Imaging for Viability Assessment of Human Liver Allografts During Normothermic Machine Perfusion |
title_sort | hyperspectral imaging for viability assessment of human liver allografts during normothermic machine perfusion |
topic | Liver Transplantation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9671746/ https://www.ncbi.nlm.nih.gov/pubmed/36406899 http://dx.doi.org/10.1097/TXD.0000000000001420 |
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