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Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach

Acinetobacter baumannii is a bacterial pathogen that is commonly associated with hospital-acquired illnesses. Antimicrobial drug resistance in A. baumannii includes several penicillin classes, first and second-generation cephalosporins, cephamycins, most aminoglycosides, chloramphenicol, and tetracy...

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Detalles Bibliográficos
Autores principales: Nandi, Abhishek, Yadav, Ruchi, Singh, Aditi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672511/
https://www.ncbi.nlm.nih.gov/pubmed/36405598
http://dx.doi.org/10.3389/fmed.2022.1047752
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author Nandi, Abhishek
Yadav, Ruchi
Singh, Aditi
author_facet Nandi, Abhishek
Yadav, Ruchi
Singh, Aditi
author_sort Nandi, Abhishek
collection PubMed
description Acinetobacter baumannii is a bacterial pathogen that is commonly associated with hospital-acquired illnesses. Antimicrobial drug resistance in A. baumannii includes several penicillin classes, first and second-generation cephalosporins, cephamycins, most aminoglycosides, chloramphenicol, and tetracyclines. The recent rise in multidrug-resistant A. baumannii strains has resulted in an increase in pneumoniae associated with ventilators, urinary tract infections associated with the catheter, and bloodstream infections, all of which have increased complications in treatment, cost of treatment, and death. Small compounds known as antimicrobial peptides (AMPs) are known to have damaging effects on pathogenic bacteria. To determine their antimicrobial activity, AMPs are created from proteins acquired from various sources and evaluated in vitro. In the last phase of lytic cycle, bacteriophages release hydrolytic enzymes called endolysins that cleave the host’s cell wall. Due to their superior potency and specificity compared to antibiotics, lysins are used as antibacterial agents. In the present study, different types of endolysin from phages of A. baumannii were selected based on an extensive literature survey. From the PhaLP database, the sequences of the selected lysins were retrieved in FASTA format and antimicrobial peptides were found among them. With the help of available bioinformatic tools, the anti-biofilm property, anti-fungal property, cell-penetrating property, and cellular toxicity of the antimicrobial peptides were determined. Out of the fourteen antimicrobial peptides found from the eight selected endolysins of A. baumannii specific phage, eight of them has anti-biofilm property, nine of them has anti-fungal property, five of them has cell-penetrating property and all of them are non-toxic.
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spelling pubmed-96725112022-11-19 Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach Nandi, Abhishek Yadav, Ruchi Singh, Aditi Front Med (Lausanne) Medicine Acinetobacter baumannii is a bacterial pathogen that is commonly associated with hospital-acquired illnesses. Antimicrobial drug resistance in A. baumannii includes several penicillin classes, first and second-generation cephalosporins, cephamycins, most aminoglycosides, chloramphenicol, and tetracyclines. The recent rise in multidrug-resistant A. baumannii strains has resulted in an increase in pneumoniae associated with ventilators, urinary tract infections associated with the catheter, and bloodstream infections, all of which have increased complications in treatment, cost of treatment, and death. Small compounds known as antimicrobial peptides (AMPs) are known to have damaging effects on pathogenic bacteria. To determine their antimicrobial activity, AMPs are created from proteins acquired from various sources and evaluated in vitro. In the last phase of lytic cycle, bacteriophages release hydrolytic enzymes called endolysins that cleave the host’s cell wall. Due to their superior potency and specificity compared to antibiotics, lysins are used as antibacterial agents. In the present study, different types of endolysin from phages of A. baumannii were selected based on an extensive literature survey. From the PhaLP database, the sequences of the selected lysins were retrieved in FASTA format and antimicrobial peptides were found among them. With the help of available bioinformatic tools, the anti-biofilm property, anti-fungal property, cell-penetrating property, and cellular toxicity of the antimicrobial peptides were determined. Out of the fourteen antimicrobial peptides found from the eight selected endolysins of A. baumannii specific phage, eight of them has anti-biofilm property, nine of them has anti-fungal property, five of them has cell-penetrating property and all of them are non-toxic. Frontiers Media S.A. 2022-11-04 /pmc/articles/PMC9672511/ /pubmed/36405598 http://dx.doi.org/10.3389/fmed.2022.1047752 Text en Copyright © 2022 Nandi, Yadav and Singh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Nandi, Abhishek
Yadav, Ruchi
Singh, Aditi
Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach
title Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach
title_full Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach
title_fullStr Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach
title_full_unstemmed Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach
title_short Phage derived lytic peptides, a secret weapon against Acinetobacter baumannii—An in silico approach
title_sort phage derived lytic peptides, a secret weapon against acinetobacter baumannii—an in silico approach
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672511/
https://www.ncbi.nlm.nih.gov/pubmed/36405598
http://dx.doi.org/10.3389/fmed.2022.1047752
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