Cargando…
Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium
Microbial community succession during the enrichment of crude-oil-degrading bacteria was analyzed using Illumina high-throughput sequencing to guide bacterial isolation and construction of a bacterial consortium. Community change occurred in 6 days; the most abundant phylum changed from Proteobacter...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672818/ https://www.ncbi.nlm.nih.gov/pubmed/36406435 http://dx.doi.org/10.3389/fmicb.2022.1044448 |
_version_ | 1784832824801492992 |
---|---|
author | Yu, Tianfei Liu, Xiaodong Ai, Jiamin Wang, Jiamin Guo, Yidan Liu, Xinhui He, Xiaolong Deng, Zhenshan Jiang, Yingying |
author_facet | Yu, Tianfei Liu, Xiaodong Ai, Jiamin Wang, Jiamin Guo, Yidan Liu, Xinhui He, Xiaolong Deng, Zhenshan Jiang, Yingying |
author_sort | Yu, Tianfei |
collection | PubMed |
description | Microbial community succession during the enrichment of crude-oil-degrading bacteria was analyzed using Illumina high-throughput sequencing to guide bacterial isolation and construction of a bacterial consortium. Community change occurred in 6 days; the most abundant phylum changed from Proteobacteria to Actinobacteria; the most abundant genera were Dietzia and unspecified_Idiomarinaceae. Two crude oil-degrading strains, Rhodococcus sp. OS62-1 and Dietzia sp. OS33, and one weak-crude-oil-degrading strain, Pseudomonas sp. P35, were isolated. A consortium comprising Rhodococcus sp. OS62-1 and Pseudomonas sp. P35 showed the highest crude-oil-degrading efficiency, reaching 85.72 ± 3.21% within 7 days, over a wide pH range (5–11) and salinity (0–80 g·L(−1)). Consumption of saturated hydrocarbons, aromatic hydrocarbons, and resins was greater by the consortium than by a single strain, as was degradation of short-chain-alkanes (C(13)–C(17)) according to gas-chromatography. The bacterial consortium provides technical support for bioremediation of crude oil pollution. |
format | Online Article Text |
id | pubmed-9672818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96728182022-11-19 Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium Yu, Tianfei Liu, Xiaodong Ai, Jiamin Wang, Jiamin Guo, Yidan Liu, Xinhui He, Xiaolong Deng, Zhenshan Jiang, Yingying Front Microbiol Microbiology Microbial community succession during the enrichment of crude-oil-degrading bacteria was analyzed using Illumina high-throughput sequencing to guide bacterial isolation and construction of a bacterial consortium. Community change occurred in 6 days; the most abundant phylum changed from Proteobacteria to Actinobacteria; the most abundant genera were Dietzia and unspecified_Idiomarinaceae. Two crude oil-degrading strains, Rhodococcus sp. OS62-1 and Dietzia sp. OS33, and one weak-crude-oil-degrading strain, Pseudomonas sp. P35, were isolated. A consortium comprising Rhodococcus sp. OS62-1 and Pseudomonas sp. P35 showed the highest crude-oil-degrading efficiency, reaching 85.72 ± 3.21% within 7 days, over a wide pH range (5–11) and salinity (0–80 g·L(−1)). Consumption of saturated hydrocarbons, aromatic hydrocarbons, and resins was greater by the consortium than by a single strain, as was degradation of short-chain-alkanes (C(13)–C(17)) according to gas-chromatography. The bacterial consortium provides technical support for bioremediation of crude oil pollution. Frontiers Media S.A. 2022-11-04 /pmc/articles/PMC9672818/ /pubmed/36406435 http://dx.doi.org/10.3389/fmicb.2022.1044448 Text en Copyright © 2022 Yu, Liu, Ai, Wang, Guo, Liu, He, Deng and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yu, Tianfei Liu, Xiaodong Ai, Jiamin Wang, Jiamin Guo, Yidan Liu, Xinhui He, Xiaolong Deng, Zhenshan Jiang, Yingying Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium |
title | Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium |
title_full | Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium |
title_fullStr | Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium |
title_full_unstemmed | Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium |
title_short | Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium |
title_sort | microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672818/ https://www.ncbi.nlm.nih.gov/pubmed/36406435 http://dx.doi.org/10.3389/fmicb.2022.1044448 |
work_keys_str_mv | AT yutianfei microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT liuxiaodong microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT aijiamin microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT wangjiamin microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT guoyidan microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT liuxinhui microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT hexiaolong microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT dengzhenshan microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium AT jiangyingying microbialcommunitysuccessionduringcrudeoildegradingbacterialenrichmentcultivationandconstructionofadegradingconsortium |