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Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering

Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food colorant. Its relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based manufacturing. Microbia...

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Autores principales: Zhang, Lijuan, Liu, Xue, Li, Jiawei, Meng, Yonghui, Zhao, Guang-Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672881/
https://www.ncbi.nlm.nih.gov/pubmed/36438069
http://dx.doi.org/10.1016/j.synbio.2022.11.002
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author Zhang, Lijuan
Liu, Xue
Li, Jiawei
Meng, Yonghui
Zhao, Guang-Rong
author_facet Zhang, Lijuan
Liu, Xue
Li, Jiawei
Meng, Yonghui
Zhao, Guang-Rong
author_sort Zhang, Lijuan
collection PubMed
description Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food colorant. Its relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based manufacturing. Microbial bioproduction therefore represents an attractive alternative. Here, we present the construction of a Saccharomyces cerevisiae strain for betanin production. Through minimizing metabolic crosstalk, screening and modifying biosynthetic enzymes, enhancing pathway flux and optimizing fermentation conditions, a final titer of betanin of 28.7 mg/L was achieved from glucose at 25 °C in baffled shake-flask, which is the highest reported titer produced by yeast to our knowledge. This work provides a promising step towards developing synthetic yeast cell factories for de novo biosynthesis of value-added betanin and other betacyanins.
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spelling pubmed-96728812022-11-25 Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering Zhang, Lijuan Liu, Xue Li, Jiawei Meng, Yonghui Zhao, Guang-Rong Synth Syst Biotechnol Original Research Article Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food colorant. Its relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based manufacturing. Microbial bioproduction therefore represents an attractive alternative. Here, we present the construction of a Saccharomyces cerevisiae strain for betanin production. Through minimizing metabolic crosstalk, screening and modifying biosynthetic enzymes, enhancing pathway flux and optimizing fermentation conditions, a final titer of betanin of 28.7 mg/L was achieved from glucose at 25 °C in baffled shake-flask, which is the highest reported titer produced by yeast to our knowledge. This work provides a promising step towards developing synthetic yeast cell factories for de novo biosynthesis of value-added betanin and other betacyanins. KeAi Publishing 2022-11-12 /pmc/articles/PMC9672881/ /pubmed/36438069 http://dx.doi.org/10.1016/j.synbio.2022.11.002 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Research Article
Zhang, Lijuan
Liu, Xue
Li, Jiawei
Meng, Yonghui
Zhao, Guang-Rong
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering
title Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering
title_full Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering
title_fullStr Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering
title_full_unstemmed Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering
title_short Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering
title_sort improvement of betanin biosynthesis in saccharomyces cerevisiae by metabolic engineering
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672881/
https://www.ncbi.nlm.nih.gov/pubmed/36438069
http://dx.doi.org/10.1016/j.synbio.2022.11.002
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