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Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations
PURPOSE: To characterize the progression of vascular changes that occur in each retinal plexus, in three canine models of inherited retinal degeneration. METHODS: In this retrospective cohort study, we examined the retinal imaging records of 44 dogs from a research colony that had undergone optical...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Association for Research in Vision and Ophthalmology
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672900/ https://www.ncbi.nlm.nih.gov/pubmed/36378130 http://dx.doi.org/10.1167/iovs.63.12.22 |
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author | Ripolles-Garcia, Ana Chen, Yineng Sato, Yu Gray, Alexa Ying, Gui-Shuang Aguirre, Gustavo D. Beltran, William A. |
author_facet | Ripolles-Garcia, Ana Chen, Yineng Sato, Yu Gray, Alexa Ying, Gui-Shuang Aguirre, Gustavo D. Beltran, William A. |
author_sort | Ripolles-Garcia, Ana |
collection | PubMed |
description | PURPOSE: To characterize the progression of vascular changes that occur in each retinal plexus, in three canine models of inherited retinal degeneration. METHODS: In this retrospective cohort study, we examined the retinal imaging records of 44 dogs from a research colony that had undergone optical coherence tomography angiography (OCTA) imaging. Animals enrolled included crd2/NPHP5 and xlpra2/RPGR mutant dogs imaged at different stages of photoreceptor loss, as well as RHO(T4R/+) dogs after acute light-induced rod degeneration. Also included were normal controls imaged at similar ages. OCT angiograms of the superficial vascular plexus combined with the intermediate capillary plexus (SVP + ICP), and the deep capillary plexus (DCP) were analyzed using the AngioTool software to calculate vessel density and other vascular parameters. RESULTS: A reduction in vessel density was seen over time in both the SVP + ICP and DCP in all mutant dogs but was more pronounced in the DCP. Scans were subclassified based on outer nuclear layer (ONL) thinning compared to age-matched normal controls. When ONL loss was 0% to 50%, vessel density in the DCP was significantly lower than in age-matched controls. In all cases, when ONL loss exceeded 87.5%, vessel density in the SVP + ICP was significantly reduced as well. In the acute light-induced rod degeneration model, the vascular regression changes were observed mainly in the DCP. CONCLUSIONS: Vessel density reduction in dogs undergoing retinal degeneration is first detected by OCTA in the DCP, and only at later stages in the SVP + ICP. |
format | Online Article Text |
id | pubmed-9672900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-96729002022-11-19 Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations Ripolles-Garcia, Ana Chen, Yineng Sato, Yu Gray, Alexa Ying, Gui-Shuang Aguirre, Gustavo D. Beltran, William A. Invest Ophthalmol Vis Sci Retina PURPOSE: To characterize the progression of vascular changes that occur in each retinal plexus, in three canine models of inherited retinal degeneration. METHODS: In this retrospective cohort study, we examined the retinal imaging records of 44 dogs from a research colony that had undergone optical coherence tomography angiography (OCTA) imaging. Animals enrolled included crd2/NPHP5 and xlpra2/RPGR mutant dogs imaged at different stages of photoreceptor loss, as well as RHO(T4R/+) dogs after acute light-induced rod degeneration. Also included were normal controls imaged at similar ages. OCT angiograms of the superficial vascular plexus combined with the intermediate capillary plexus (SVP + ICP), and the deep capillary plexus (DCP) were analyzed using the AngioTool software to calculate vessel density and other vascular parameters. RESULTS: A reduction in vessel density was seen over time in both the SVP + ICP and DCP in all mutant dogs but was more pronounced in the DCP. Scans were subclassified based on outer nuclear layer (ONL) thinning compared to age-matched normal controls. When ONL loss was 0% to 50%, vessel density in the DCP was significantly lower than in age-matched controls. In all cases, when ONL loss exceeded 87.5%, vessel density in the SVP + ICP was significantly reduced as well. In the acute light-induced rod degeneration model, the vascular regression changes were observed mainly in the DCP. CONCLUSIONS: Vessel density reduction in dogs undergoing retinal degeneration is first detected by OCTA in the DCP, and only at later stages in the SVP + ICP. The Association for Research in Vision and Ophthalmology 2022-11-15 /pmc/articles/PMC9672900/ /pubmed/36378130 http://dx.doi.org/10.1167/iovs.63.12.22 Text en Copyright 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Retina Ripolles-Garcia, Ana Chen, Yineng Sato, Yu Gray, Alexa Ying, Gui-Shuang Aguirre, Gustavo D. Beltran, William A. Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations |
title | Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations |
title_full | Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations |
title_fullStr | Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations |
title_full_unstemmed | Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations |
title_short | Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations |
title_sort | retinal vascular plexuses are unequally affected in canine inherited retinal degenerations |
topic | Retina |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672900/ https://www.ncbi.nlm.nih.gov/pubmed/36378130 http://dx.doi.org/10.1167/iovs.63.12.22 |
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