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Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations

PURPOSE: To characterize the progression of vascular changes that occur in each retinal plexus, in three canine models of inherited retinal degeneration. METHODS: In this retrospective cohort study, we examined the retinal imaging records of 44 dogs from a research colony that had undergone optical...

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Autores principales: Ripolles-Garcia, Ana, Chen, Yineng, Sato, Yu, Gray, Alexa, Ying, Gui-Shuang, Aguirre, Gustavo D., Beltran, William A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672900/
https://www.ncbi.nlm.nih.gov/pubmed/36378130
http://dx.doi.org/10.1167/iovs.63.12.22
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author Ripolles-Garcia, Ana
Chen, Yineng
Sato, Yu
Gray, Alexa
Ying, Gui-Shuang
Aguirre, Gustavo D.
Beltran, William A.
author_facet Ripolles-Garcia, Ana
Chen, Yineng
Sato, Yu
Gray, Alexa
Ying, Gui-Shuang
Aguirre, Gustavo D.
Beltran, William A.
author_sort Ripolles-Garcia, Ana
collection PubMed
description PURPOSE: To characterize the progression of vascular changes that occur in each retinal plexus, in three canine models of inherited retinal degeneration. METHODS: In this retrospective cohort study, we examined the retinal imaging records of 44 dogs from a research colony that had undergone optical coherence tomography angiography (OCTA) imaging. Animals enrolled included crd2/NPHP5 and xlpra2/RPGR mutant dogs imaged at different stages of photoreceptor loss, as well as RHO(T4R/+) dogs after acute light-induced rod degeneration. Also included were normal controls imaged at similar ages. OCT angiograms of the superficial vascular plexus combined with the intermediate capillary plexus (SVP + ICP), and the deep capillary plexus (DCP) were analyzed using the AngioTool software to calculate vessel density and other vascular parameters. RESULTS: A reduction in vessel density was seen over time in both the SVP + ICP and DCP in all mutant dogs but was more pronounced in the DCP. Scans were subclassified based on outer nuclear layer (ONL) thinning compared to age-matched normal controls. When ONL loss was 0% to 50%, vessel density in the DCP was significantly lower than in age-matched controls. In all cases, when ONL loss exceeded 87.5%, vessel density in the SVP + ICP was significantly reduced as well. In the acute light-induced rod degeneration model, the vascular regression changes were observed mainly in the DCP. CONCLUSIONS: Vessel density reduction in dogs undergoing retinal degeneration is first detected by OCTA in the DCP, and only at later stages in the SVP + ICP.
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spelling pubmed-96729002022-11-19 Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations Ripolles-Garcia, Ana Chen, Yineng Sato, Yu Gray, Alexa Ying, Gui-Shuang Aguirre, Gustavo D. Beltran, William A. Invest Ophthalmol Vis Sci Retina PURPOSE: To characterize the progression of vascular changes that occur in each retinal plexus, in three canine models of inherited retinal degeneration. METHODS: In this retrospective cohort study, we examined the retinal imaging records of 44 dogs from a research colony that had undergone optical coherence tomography angiography (OCTA) imaging. Animals enrolled included crd2/NPHP5 and xlpra2/RPGR mutant dogs imaged at different stages of photoreceptor loss, as well as RHO(T4R/+) dogs after acute light-induced rod degeneration. Also included were normal controls imaged at similar ages. OCT angiograms of the superficial vascular plexus combined with the intermediate capillary plexus (SVP + ICP), and the deep capillary plexus (DCP) were analyzed using the AngioTool software to calculate vessel density and other vascular parameters. RESULTS: A reduction in vessel density was seen over time in both the SVP + ICP and DCP in all mutant dogs but was more pronounced in the DCP. Scans were subclassified based on outer nuclear layer (ONL) thinning compared to age-matched normal controls. When ONL loss was 0% to 50%, vessel density in the DCP was significantly lower than in age-matched controls. In all cases, when ONL loss exceeded 87.5%, vessel density in the SVP + ICP was significantly reduced as well. In the acute light-induced rod degeneration model, the vascular regression changes were observed mainly in the DCP. CONCLUSIONS: Vessel density reduction in dogs undergoing retinal degeneration is first detected by OCTA in the DCP, and only at later stages in the SVP + ICP. The Association for Research in Vision and Ophthalmology 2022-11-15 /pmc/articles/PMC9672900/ /pubmed/36378130 http://dx.doi.org/10.1167/iovs.63.12.22 Text en Copyright 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Retina
Ripolles-Garcia, Ana
Chen, Yineng
Sato, Yu
Gray, Alexa
Ying, Gui-Shuang
Aguirre, Gustavo D.
Beltran, William A.
Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations
title Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations
title_full Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations
title_fullStr Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations
title_full_unstemmed Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations
title_short Retinal Vascular Plexuses Are Unequally Affected in Canine Inherited Retinal Degenerations
title_sort retinal vascular plexuses are unequally affected in canine inherited retinal degenerations
topic Retina
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672900/
https://www.ncbi.nlm.nih.gov/pubmed/36378130
http://dx.doi.org/10.1167/iovs.63.12.22
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