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Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link

Bauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavai...

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Autores principales: Verônica Cardoso de Souza, Bárbara, de Morais Sousa, Mariana, Augusto Gasparotto Sattler, José, Cristina Sousa Gramoza Vilarinho Santana, Ana, Bruno Fonseca de Carvalho, Rusbene, de Sousa Lima Neto, José, de Matos Borges, Fernando, Angelica Neri Numa, Iramaia, Braga Ribeiro, Alessandra, César Cunha Nunes, Lívio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672949/
https://www.ncbi.nlm.nih.gov/pubmed/36404894
http://dx.doi.org/10.1016/j.fochms.2022.100144
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author Verônica Cardoso de Souza, Bárbara
de Morais Sousa, Mariana
Augusto Gasparotto Sattler, José
Cristina Sousa Gramoza Vilarinho Santana, Ana
Bruno Fonseca de Carvalho, Rusbene
de Sousa Lima Neto, José
de Matos Borges, Fernando
Angelica Neri Numa, Iramaia
Braga Ribeiro, Alessandra
César Cunha Nunes, Lívio
author_facet Verônica Cardoso de Souza, Bárbara
de Morais Sousa, Mariana
Augusto Gasparotto Sattler, José
Cristina Sousa Gramoza Vilarinho Santana, Ana
Bruno Fonseca de Carvalho, Rusbene
de Sousa Lima Neto, José
de Matos Borges, Fernando
Angelica Neri Numa, Iramaia
Braga Ribeiro, Alessandra
César Cunha Nunes, Lívio
author_sort Verônica Cardoso de Souza, Bárbara
collection PubMed
description Bauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of B. forficata Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MS(n), evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (<0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated in vitro digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of B. forficata is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets.
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spelling pubmed-96729492022-11-19 Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link Verônica Cardoso de Souza, Bárbara de Morais Sousa, Mariana Augusto Gasparotto Sattler, José Cristina Sousa Gramoza Vilarinho Santana, Ana Bruno Fonseca de Carvalho, Rusbene de Sousa Lima Neto, José de Matos Borges, Fernando Angelica Neri Numa, Iramaia Braga Ribeiro, Alessandra César Cunha Nunes, Lívio Food Chem (Oxf) Article Bauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of B. forficata Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MS(n), evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (<0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated in vitro digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of B. forficata is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets. Elsevier 2022-11-07 /pmc/articles/PMC9672949/ /pubmed/36404894 http://dx.doi.org/10.1016/j.fochms.2022.100144 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Verônica Cardoso de Souza, Bárbara
de Morais Sousa, Mariana
Augusto Gasparotto Sattler, José
Cristina Sousa Gramoza Vilarinho Santana, Ana
Bruno Fonseca de Carvalho, Rusbene
de Sousa Lima Neto, José
de Matos Borges, Fernando
Angelica Neri Numa, Iramaia
Braga Ribeiro, Alessandra
César Cunha Nunes, Lívio
Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link
title Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link
title_full Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link
title_fullStr Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link
title_full_unstemmed Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link
title_short Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link
title_sort nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from bauhinia forficata link
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9672949/
https://www.ncbi.nlm.nih.gov/pubmed/36404894
http://dx.doi.org/10.1016/j.fochms.2022.100144
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