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Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt

Bacterial communities in the rhizosphere play an important role in sustaining plant growth and the health of diverse soils. Recent studies have demonstrated that microbial keystone taxa in the rhizosphere microbial community are extremely critical for the suppression of diseases. However, the mechan...

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Autores principales: Shang, Xian-chao, Cai, Xianjie, Zhou, Yanan, Han, Xiaobin, Zhang, Cheng-Sheng, Ilyas, Naila, Li, Yiqiang, Zheng, Yanfen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673043/
https://www.ncbi.nlm.nih.gov/pubmed/36406638
http://dx.doi.org/10.3389/fpls.2021.738611
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author Shang, Xian-chao
Cai, Xianjie
Zhou, Yanan
Han, Xiaobin
Zhang, Cheng-Sheng
Ilyas, Naila
Li, Yiqiang
Zheng, Yanfen
author_facet Shang, Xian-chao
Cai, Xianjie
Zhou, Yanan
Han, Xiaobin
Zhang, Cheng-Sheng
Ilyas, Naila
Li, Yiqiang
Zheng, Yanfen
author_sort Shang, Xian-chao
collection PubMed
description Bacterial communities in the rhizosphere play an important role in sustaining plant growth and the health of diverse soils. Recent studies have demonstrated that microbial keystone taxa in the rhizosphere microbial community are extremely critical for the suppression of diseases. However, the mechanisms involved in disease suppression by keystone species remain unclear. The present study assessed the effects of three Pseudomonas strains, which were identified as keystone species in our previous study, on the growth performance and root-associated bacterial community of tobacco plants. A high relative abundance of Ralstonia was found in the non-inoculated group, while a large Azospira population was observed in all groups inoculated with the three Pseudomonas strains. Correspondingly, the activities of the defense-related enzymes and the expression levels of the defense signaling marker genes of the plant were increased after inoculation with the Pseudomonas strains. Moreover, the correlation analyses showed that the relative abundance of Azospira, the activity of superoxide dismutase, catalase, and polyphenol oxidase, and the expression of H1N1, ACC Oxidase, and PR1 a/c had a significantly negative (p<0.05) relationship with the abundance of Ralstonia. This further revealed that the keystone species, such as Pseudomonas spp., can suppress bacterial wilt disease by enhancing the systemic resistance of tobacco plants.
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spelling pubmed-96730432022-11-19 Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt Shang, Xian-chao Cai, Xianjie Zhou, Yanan Han, Xiaobin Zhang, Cheng-Sheng Ilyas, Naila Li, Yiqiang Zheng, Yanfen Front Plant Sci Plant Science Bacterial communities in the rhizosphere play an important role in sustaining plant growth and the health of diverse soils. Recent studies have demonstrated that microbial keystone taxa in the rhizosphere microbial community are extremely critical for the suppression of diseases. However, the mechanisms involved in disease suppression by keystone species remain unclear. The present study assessed the effects of three Pseudomonas strains, which were identified as keystone species in our previous study, on the growth performance and root-associated bacterial community of tobacco plants. A high relative abundance of Ralstonia was found in the non-inoculated group, while a large Azospira population was observed in all groups inoculated with the three Pseudomonas strains. Correspondingly, the activities of the defense-related enzymes and the expression levels of the defense signaling marker genes of the plant were increased after inoculation with the Pseudomonas strains. Moreover, the correlation analyses showed that the relative abundance of Azospira, the activity of superoxide dismutase, catalase, and polyphenol oxidase, and the expression of H1N1, ACC Oxidase, and PR1 a/c had a significantly negative (p<0.05) relationship with the abundance of Ralstonia. This further revealed that the keystone species, such as Pseudomonas spp., can suppress bacterial wilt disease by enhancing the systemic resistance of tobacco plants. Frontiers Media S.A. 2021-09-22 /pmc/articles/PMC9673043/ /pubmed/36406638 http://dx.doi.org/10.3389/fpls.2021.738611 Text en Copyright © 2021 Shang, Cai, Zhou, Han, Zhang, Ilyas, Li and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shang, Xian-chao
Cai, Xianjie
Zhou, Yanan
Han, Xiaobin
Zhang, Cheng-Sheng
Ilyas, Naila
Li, Yiqiang
Zheng, Yanfen
Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt
title Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt
title_full Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt
title_fullStr Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt
title_full_unstemmed Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt
title_short Pseudomonas Inoculation Stimulates Endophytic Azospira Population and Induces Systemic Resistance to Bacterial Wilt
title_sort pseudomonas inoculation stimulates endophytic azospira population and induces systemic resistance to bacterial wilt
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673043/
https://www.ncbi.nlm.nih.gov/pubmed/36406638
http://dx.doi.org/10.3389/fpls.2021.738611
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