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On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene
Furunculosis, which is caused by Aeromonas salmonicida, can induce septicemia, leading to the rapid death of fishes belonging to Salmonidae, Cyprinidae, and Fuscheridae, and lamprey. Targeting A. salmonicida, five DNAzyme sequences with the highest enrichment rates were selected through the Systemat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673123/ https://www.ncbi.nlm.nih.gov/pubmed/36405323 http://dx.doi.org/10.3389/fchem.2022.1015827 |
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author | Ding, Wen Miao, Qingzhen Bao, Xiuli Wang, Shiyuan Lu, Jing Lyu, Mingsheng Wang, Shujun |
author_facet | Ding, Wen Miao, Qingzhen Bao, Xiuli Wang, Shiyuan Lu, Jing Lyu, Mingsheng Wang, Shujun |
author_sort | Ding, Wen |
collection | PubMed |
description | Furunculosis, which is caused by Aeromonas salmonicida, can induce septicemia, leading to the rapid death of fishes belonging to Salmonidae, Cyprinidae, and Fuscheridae, and lamprey. Targeting A. salmonicida, five DNAzyme sequences with the highest enrichment rates were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX). The enrichment rates were 34.78, 23.60, 8.91, 2.89, and 2.34%, respectively. The DNAzyme with the highest activity, named D-AS-2, showed specificity and sensitivity. D-AS-2 was combined with carboxyl-functionalized graphene to construct a biosensor, which showed good fluorescence response to scabies lesion samples. The diagnostic procedure was completed in <2 min and can be used for the on-site diagnosis of fish diseases. A low-cost, rapid, simple, and highly specific biosensor for the diagnosis of furunculosis was established based on DNAzyme and carboxyl-functionalized graphene. |
format | Online Article Text |
id | pubmed-9673123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96731232022-11-19 On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene Ding, Wen Miao, Qingzhen Bao, Xiuli Wang, Shiyuan Lu, Jing Lyu, Mingsheng Wang, Shujun Front Chem Chemistry Furunculosis, which is caused by Aeromonas salmonicida, can induce septicemia, leading to the rapid death of fishes belonging to Salmonidae, Cyprinidae, and Fuscheridae, and lamprey. Targeting A. salmonicida, five DNAzyme sequences with the highest enrichment rates were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX). The enrichment rates were 34.78, 23.60, 8.91, 2.89, and 2.34%, respectively. The DNAzyme with the highest activity, named D-AS-2, showed specificity and sensitivity. D-AS-2 was combined with carboxyl-functionalized graphene to construct a biosensor, which showed good fluorescence response to scabies lesion samples. The diagnostic procedure was completed in <2 min and can be used for the on-site diagnosis of fish diseases. A low-cost, rapid, simple, and highly specific biosensor for the diagnosis of furunculosis was established based on DNAzyme and carboxyl-functionalized graphene. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9673123/ /pubmed/36405323 http://dx.doi.org/10.3389/fchem.2022.1015827 Text en Copyright © 2022 Ding, Miao, Bao, Wang, Lu, Lyu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Ding, Wen Miao, Qingzhen Bao, Xiuli Wang, Shiyuan Lu, Jing Lyu, Mingsheng Wang, Shujun On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene |
title | On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene |
title_full | On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene |
title_fullStr | On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene |
title_full_unstemmed | On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene |
title_short | On-site detection of fish furunculosis by combining DNAzyme and carboxyl-functionalized graphene |
title_sort | on-site detection of fish furunculosis by combining dnazyme and carboxyl-functionalized graphene |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673123/ https://www.ncbi.nlm.nih.gov/pubmed/36405323 http://dx.doi.org/10.3389/fchem.2022.1015827 |
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