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Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow

Bovine papillomavirus (BPV) infection can induce neoplastic lesions in both cutaneous and mucosal epithelia in cattle. This study describes the BPV types associated with proliferative lesions with diverse histopathological features present in the upper alimentary tract of a dairy cow suffering from...

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Autores principales: Fernandes, Kamila Peruchi, Alfieri, Amauri Alcindo, Darold, Gabriela Molinari, Boabaid, Fabiana Marques, Dall Agnol, Alais Maria, Lunardi, Michele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673478/
https://www.ncbi.nlm.nih.gov/pubmed/36406071
http://dx.doi.org/10.3389/fvets.2022.1020166
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author Fernandes, Kamila Peruchi
Alfieri, Amauri Alcindo
Darold, Gabriela Molinari
Boabaid, Fabiana Marques
Dall Agnol, Alais Maria
Lunardi, Michele
author_facet Fernandes, Kamila Peruchi
Alfieri, Amauri Alcindo
Darold, Gabriela Molinari
Boabaid, Fabiana Marques
Dall Agnol, Alais Maria
Lunardi, Michele
author_sort Fernandes, Kamila Peruchi
collection PubMed
description Bovine papillomavirus (BPV) infection can induce neoplastic lesions in both cutaneous and mucosal epithelia in cattle. This study describes the BPV types associated with proliferative lesions with diverse histopathological features present in the upper alimentary tract of a dairy cow suffering from chronic diarrhea from Midwestern Brazil. At autopsy, warts and plaques composed of multiple spherical nodules were observed in the esophageal mucosa, the areas surrounding and constricting the opening of the cardia, and the rumen pillars. One esophageal papillomatous proliferative lesion and a smooth-surfaced proliferative lesion located at the rumen entrance were evaluated by histopathological and molecular analyses. PCR amplification of partial fragments of the BPV L1 and E1 genes was performed followed by sequencing of the obtained amplicons. Upon histopathological evaluation, the esophageal lesion was classified as a squamous papilloma, whereas the other ruminal proliferative lesion consisted of a fibropapilloma. Direct sequencing of PCR products obtained from ruminal fibropapilloma DNA revealed the presence of BPV2. Sequencing of inserts from selected clones containing partial fragments of the BPV L1 and E1 genes revealed a mixed infection of BPV types 2 and 4 in the esophageal squamous papilloma. The findings reported in our investigation reinforce the association of BPV with benign lesions of the bovine alimentary tract in both single and mixed infections, as previously demonstrated to occur in a buffalo. In addition, this report represents the documentation of the occurrence of massive alimentary papillomatosis associated with BPV types 2 and 4 in cattle raised on lands without infestation by bracken fern in Midwestern Brazil.
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spelling pubmed-96734782022-11-19 Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow Fernandes, Kamila Peruchi Alfieri, Amauri Alcindo Darold, Gabriela Molinari Boabaid, Fabiana Marques Dall Agnol, Alais Maria Lunardi, Michele Front Vet Sci Veterinary Science Bovine papillomavirus (BPV) infection can induce neoplastic lesions in both cutaneous and mucosal epithelia in cattle. This study describes the BPV types associated with proliferative lesions with diverse histopathological features present in the upper alimentary tract of a dairy cow suffering from chronic diarrhea from Midwestern Brazil. At autopsy, warts and plaques composed of multiple spherical nodules were observed in the esophageal mucosa, the areas surrounding and constricting the opening of the cardia, and the rumen pillars. One esophageal papillomatous proliferative lesion and a smooth-surfaced proliferative lesion located at the rumen entrance were evaluated by histopathological and molecular analyses. PCR amplification of partial fragments of the BPV L1 and E1 genes was performed followed by sequencing of the obtained amplicons. Upon histopathological evaluation, the esophageal lesion was classified as a squamous papilloma, whereas the other ruminal proliferative lesion consisted of a fibropapilloma. Direct sequencing of PCR products obtained from ruminal fibropapilloma DNA revealed the presence of BPV2. Sequencing of inserts from selected clones containing partial fragments of the BPV L1 and E1 genes revealed a mixed infection of BPV types 2 and 4 in the esophageal squamous papilloma. The findings reported in our investigation reinforce the association of BPV with benign lesions of the bovine alimentary tract in both single and mixed infections, as previously demonstrated to occur in a buffalo. In addition, this report represents the documentation of the occurrence of massive alimentary papillomatosis associated with BPV types 2 and 4 in cattle raised on lands without infestation by bracken fern in Midwestern Brazil. Frontiers Media S.A. 2022-11-04 /pmc/articles/PMC9673478/ /pubmed/36406071 http://dx.doi.org/10.3389/fvets.2022.1020166 Text en Copyright © 2022 Fernandes, Alfieri, Darold, Boabaid, Dall Agnol and Lunardi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Fernandes, Kamila Peruchi
Alfieri, Amauri Alcindo
Darold, Gabriela Molinari
Boabaid, Fabiana Marques
Dall Agnol, Alais Maria
Lunardi, Michele
Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
title Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
title_full Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
title_fullStr Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
title_full_unstemmed Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
title_short Case report: Mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
title_sort case report: mixed infection of bovine papillomaviruses associated with squamous papilloma of the upper alimentary tract in a dairy cow
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673478/
https://www.ncbi.nlm.nih.gov/pubmed/36406071
http://dx.doi.org/10.3389/fvets.2022.1020166
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