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Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells

Globularia alypum (GA), a plant of the Globulariacea family, has long been used as a traditional cure for inflammatory and metabolic illnesses. In addition to various in vitro model studies, the current work focuses on the antioxidant and anti-inflammatory properties of GA in human colon biopsies. T...

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Autores principales: Hajji, Najla, Hudik, Elodie, Iovino, Paola, Zouari, Nacim, Sebai, Hichem, Nusse, Oliver, Ciacci, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Università di Salerno 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673913/
https://www.ncbi.nlm.nih.gov/pubmed/36447742
http://dx.doi.org/10.37825/2239-9754.1030
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author Hajji, Najla
Hudik, Elodie
Iovino, Paola
Zouari, Nacim
Sebai, Hichem
Nusse, Oliver
Ciacci, Carolina
author_facet Hajji, Najla
Hudik, Elodie
Iovino, Paola
Zouari, Nacim
Sebai, Hichem
Nusse, Oliver
Ciacci, Carolina
author_sort Hajji, Najla
collection PubMed
description Globularia alypum (GA), a plant of the Globulariacea family, has long been used as a traditional cure for inflammatory and metabolic illnesses. In addition to various in vitro model studies, the current work focuses on the antioxidant and anti-inflammatory properties of GA in human colon biopsies. The phenol components in GA aqueous extract (GAAE) were identified by Liquid Chromatography-Electrospray Ionization Mass Spectrometry. The antioxidant ability of GAAE was tested in vitro utilizing chemiluminescence and flow cytometry using fluorescent yeasts n conjunction with PLB-985-human myeloid leukemia cells. Experiments on human colon biopsies after a biopsy challenge with Escherichia coli-lipopolysaccharides aimed to see if GAAE had an anti-inflammatory impact on human colon inflammation. Western blotting was used to assess the expression of several inflammatory markers. According to the findings, GAAE had a significant influence on hydrogen peroxide and cellular reactive oxygen species. GAAE inhibited the activities of cyclooxygenase 2 and nuclear factor B in inflamed biopsies, indicating anti-inflammatory action. The present study is the first to show that GA has a beneficial effect on human colon inflammation, thanks to its significant antioxidant activity in vitro. According to these preliminary data, GA may be utilized to treat a range of human inflammatory illnesses.
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spelling pubmed-96739132022-11-28 Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells Hajji, Najla Hudik, Elodie Iovino, Paola Zouari, Nacim Sebai, Hichem Nusse, Oliver Ciacci, Carolina Transl Med UniSa Original Article Globularia alypum (GA), a plant of the Globulariacea family, has long been used as a traditional cure for inflammatory and metabolic illnesses. In addition to various in vitro model studies, the current work focuses on the antioxidant and anti-inflammatory properties of GA in human colon biopsies. The phenol components in GA aqueous extract (GAAE) were identified by Liquid Chromatography-Electrospray Ionization Mass Spectrometry. The antioxidant ability of GAAE was tested in vitro utilizing chemiluminescence and flow cytometry using fluorescent yeasts n conjunction with PLB-985-human myeloid leukemia cells. Experiments on human colon biopsies after a biopsy challenge with Escherichia coli-lipopolysaccharides aimed to see if GAAE had an anti-inflammatory impact on human colon inflammation. Western blotting was used to assess the expression of several inflammatory markers. According to the findings, GAAE had a significant influence on hydrogen peroxide and cellular reactive oxygen species. GAAE inhibited the activities of cyclooxygenase 2 and nuclear factor B in inflamed biopsies, indicating anti-inflammatory action. The present study is the first to show that GA has a beneficial effect on human colon inflammation, thanks to its significant antioxidant activity in vitro. According to these preliminary data, GA may be utilized to treat a range of human inflammatory illnesses. Università di Salerno 2021-12-23 /pmc/articles/PMC9673913/ /pubmed/36447742 http://dx.doi.org/10.37825/2239-9754.1030 Text en © 2021 Università di Salerno. https://creativecommons.org/licenses/by/2.5/This is an open access article under the CC BY 2.5 license (https://creativecommons.org/licenses/by/2.5/).
spellingShingle Original Article
Hajji, Najla
Hudik, Elodie
Iovino, Paola
Zouari, Nacim
Sebai, Hichem
Nusse, Oliver
Ciacci, Carolina
Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells
title Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells
title_full Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells
title_fullStr Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells
title_full_unstemmed Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells
title_short Globularia alypum L. Modulates Inflammatory Markers in Human Colon and Shows a Potential Antioxidant Role in Myeloid Leukemic Cells
title_sort globularia alypum l. modulates inflammatory markers in human colon and shows a potential antioxidant role in myeloid leukemic cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673913/
https://www.ncbi.nlm.nih.gov/pubmed/36447742
http://dx.doi.org/10.37825/2239-9754.1030
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