An optimized protocol for immuno-electron microscopy of endogenous LC3

MAP1LC3/LC3 (microtubule associated protein 1 light chain 3) is widely used as marker of autophagic compartments at different stages of maturation. Electron microscopy (EM) combined with immunolabeling is the only technique that can reveal the ultrastructural identity of LC3-labeled compartments. Ho...

Descripción completa

Detalles Bibliográficos
Autores principales: De Mazière, Ann, van der Beek, Jan, van Dijk, Suzanne, de Heus, Cecilia, Reggiori, Fulvio, Koike, Masato, Klumperman, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Toolbox
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673964/
https://www.ncbi.nlm.nih.gov/pubmed/35387562
http://dx.doi.org/10.1080/15548627.2022.2056864

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673964/
https://www.ncbi.nlm.nih.gov/pubmed/35387562
http://dx.doi.org/10.1080/15548627.2022.2056864

Ejemplares similares