Cargando…

An optimized protocol for immuno-electron microscopy of endogenous LC3

MAP1LC3/LC3 (microtubule associated protein 1 light chain 3) is widely used as marker of autophagic compartments at different stages of maturation. Electron microscopy (EM) combined with immunolabeling is the only technique that can reveal the ultrastructural identity of LC3-labeled compartments. Ho...

Descripción completa

Detalles Bibliográficos
Autores principales:	De Mazière, Ann, van der Beek, Jan, van Dijk, Suzanne, de Heus, Cecilia, Reggiori, Fulvio, Koike, Masato, Klumperman, Judith
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2022
Materias:	Toolbox
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673964/ https://www.ncbi.nlm.nih.gov/pubmed/35387562 http://dx.doi.org/10.1080/15548627.2022.2056864

Ejemplares similares

Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins
por: van der Beek, Jan, et al.
Publicado: (2021)

Correlative light and electron microscopy imaging of autophagy in a zebrafish infection model
por: Hosseini, Rohola, et al.
Publicado: (2014)

High Data Output and Automated 3D Correlative Light–Electron Microscopy Method
por: Vicidomini, Giuseppe, et al.
Publicado: (2008)

Correlative single‐molecule localization microscopy and electron tomography reveals endosome nanoscale domains
por: Franke, Christian, et al.
Publicado: (2019)

Monitoring basal autophagy in the retina utilizing CAG-mRFP-EGFP-MAP1LC3B reporter mouse: technical and biological considerations
por: Ramachandra Rao, Sriganesh, et al.
Publicado: (2021)

Multiparameter phenotypic screening for endogenous TFEB and TFE3 translocation identifies novel chemical series modulating lysosome function
por: Carling, Phillippa J, et al.
Publicado: (2022)

Implantation, orientation and validation of a commercially produced heart-rate logger for use in a perciform teleost fish
por: Muller, Cuen, et al.
Publicado: (2020)

Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish
por: Andrewartha, Sarah J., et al.
Publicado: (2016)

Evaluation of different temporal periods between hormone-induced ovulation attempts in the female Fowler’s toad Anaxyrus fowleri
por: Guy, Emmet L, et al.
Publicado: (2020)

Quantitative analysis of autophagic flux by confocal pH-imaging of autophagic intermediates
por: Maulucci, Giuseppe, et al.
Publicado: (2015)

Non-invasive methods to measure inter-renal function in aquatic salamanders—correlating fecal corticosterone to the environmental and physiologic conditions of captive Necturus
por: Nagel, Andrew H, et al.
Publicado: (2019)

A novel Atg5-shRNA mouse model enables temporal control of Autophagy in vivo
por: Cassidy, Liam D., et al.
Publicado: (2018)

Sensitive ELISA-based detection method for the mitophagy marker p-S65-Ub in human cells, autopsy brain, and blood samples
por: Watzlawik, Jens O., et al.
Publicado: (2020)

A high-throughput FRET-based assay for determination of Atg4 activity
por: Li, Min, et al.
Publicado: (2012)

Semi-Automated Analysis of Organelle Movement and Membrane Content: Understanding Rab-Motor Complex Transport Function
por: Hume, Alistair N, et al.
Publicado: (2011)

A novel method for autophagy detection in primary cells: Impaired levels of macroautophagy in immunosenescent T cells
por: Phadwal, Kanchan, et al.
Publicado: (2012)

Live imaging and single-cell analysis reveal differential dynamics of autophagy and apoptosis
por: Xu, Yangqing, et al.
Publicado: (2013)

MitoTimer probe reveals the impact of autophagy, fusion, and motility on subcellular distribution of young and old mitochondrial protein and on relative mitochondrial protein age
por: Ferree, Andrew W, et al.
Publicado: (2013)

Dissecting Functions of the Conserved Oligomeric Golgi Tethering Complex Using a Cell-Free Assay
por: Cottam, Nathanael P, et al.
Publicado: (2014)

Protein Ligation in Living Cells Using Sortase
por: Strijbis, Karin, et al.
Publicado: (2012)

A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications
por: Guo, Sujuan, et al.
Publicado: (2015)

Development and application of an antibody-based protein microarray to assess physiological stress in grizzly bears (Ursus arctos)
por: Carlson, Ruth I., et al.
Publicado: (2016)

Shotgun proteomics as a viable approach for biological discovery in the Pacific oyster
por: Timmins-Schiffman, Emma, et al.
Publicado: (2013)

Enzyme immunoassays as a method for quantifying hair reproductive hormones in two felid species
por: Terwissen, C. V., et al.
Publicado: (2014)

A practical field extraction method for non-invasive monitoring of hormone activity in the black rhinoceros
por: Edwards, Katie L., et al.
Publicado: (2014)

Effects of natural environmental conditions on faecal glucocorticoid metabolite concentrations in jaguars (Panthera onca) in Belize
por: Mesa-Cruz, J. Bernardo, et al.
Publicado: (2014)

Measurement of free glucocorticoids: quantifying corticosteroid-binding globulin binding affinity and its variation within and among mammalian species
por: Delehanty, Brendan, et al.
Publicado: (2015)

Using novel methodologies to examine the impact of artificial light at night on the cortisol stress response in dispersing Atlantic salmon (Salmo salar L.) fry
por: Newman, Rhian C., et al.
Publicado: (2015)

Developing a new research tool for use in free-ranging cetaceans: recovering cortisol from harbour porpoise skin
por: Bechshoft, Thea, et al.
Publicado: (2015)

Longitudinal progesterone profiles in baleen from female North Atlantic right whales (Eubalaena glacialis) match known calving history
por: Hunt, Kathleen E., et al.
Publicado: (2016)

Baleen hormones: a novel tool for retrospective assessment of stress and reproduction in bowhead whales (Balaena mysticetus)
por: Hunt, Kathleen E., et al.
Publicado: (2014)

Temporal overlap and repeatability of feather corticosterone levels: practical considerations for use as a biomarker
por: Harris, Christopher M., et al.
Publicado: (2016)

An evaluation of the use of pentosidine as a biomarker for ageing turtles
por: Iverson, John B., et al.
Publicado: (2017)

Effects of post-mortem storage conditions of bovine epididymides on sperm characteristics: investigating a tool for preservation of sperm from endangered species
por: Strand, Julie, et al.
Publicado: (2016)

Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser
por: Hamilton, Matthew T., et al.
Publicado: (2016)

Using HHsearch to tackle proteins of unknown function: A pilot study with PH domains
por: Fidler, David R., et al.
Publicado: (2016)

Three‐dimensional immersive virtual reality for studying cellular compartments in 3D models from EM preparations of neural tissues
por: Calì, Corrado, et al.
Publicado: (2016)

Get the most out of blow hormones: validation of sampling materials, field storage and extraction techniques for whale respiratory vapour samples
por: Burgess, Elizabeth A., et al.
Publicado: (2016)

Brain maps 4.0—Structure of the rat brain: An open access atlas with global nervous system nomenclature ontology and flatmaps
por: Swanson, Larry W.
Publicado: (2018)

Semen collection and ejaculate characteristics of the Leopard Tortoise (Stigmochelys pardalis)
por: Zimmerman, Dawn M, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_srvvj666iv04ie9go1a82jputd