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Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D
Strawberry gray mold caused by Botrytis cinerea is one of the most severe diseases in pre- and post-harvest periods. Although fungicides have been an effective way to control this disease, they can cause serious “3R” problems (Resistance, Resurgence and Residue). In this study, Streptomyces sp. sdu1...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9674021/ https://www.ncbi.nlm.nih.gov/pubmed/36406410 http://dx.doi.org/10.3389/fmicb.2022.1051730 |
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author | Yong, Daojing Li, Yue Gong, Kai Yu, Yingying Zhao, Shuai Duan, Qiong Ren, Cailing Li, Aiying Fu, Jun Ni, Jinfeng Zhang, Youming Li, Ruijuan |
author_facet | Yong, Daojing Li, Yue Gong, Kai Yu, Yingying Zhao, Shuai Duan, Qiong Ren, Cailing Li, Aiying Fu, Jun Ni, Jinfeng Zhang, Youming Li, Ruijuan |
author_sort | Yong, Daojing |
collection | PubMed |
description | Strawberry gray mold caused by Botrytis cinerea is one of the most severe diseases in pre- and post-harvest periods. Although fungicides have been an effective way to control this disease, they can cause serious “3R” problems (Resistance, Resurgence and Residue). In this study, Streptomyces sp. sdu1201 isolated from the hindgut of the fungus-growing termite Odontotermes formosanus revealed significant antifungal activity against B. cinerea. Four compounds (1–4) were isolated from Streptomyces sp. sdu1201 and further identified as actinomycins by the HRMS and 1D NMR data. Among them, actinomycin D had the strongest inhibitory activity against B. cinerea with the EC(50) value of 7.65 μg mL(−1). The control effect of actinomycin D on strawberry gray mold was also tested on fruits and leaves in vitro, and its control efficiency on leaves was 78.77% at 3 d. Moreover, actinomycin D can also inhibit the polarized growth of germ tubes of B. cinerea. Therefore, Streptomyces sp. sdu1201 and actinomycin D have great potential to gray mold as biocontrol agents. |
format | Online Article Text |
id | pubmed-9674021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96740212022-11-19 Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D Yong, Daojing Li, Yue Gong, Kai Yu, Yingying Zhao, Shuai Duan, Qiong Ren, Cailing Li, Aiying Fu, Jun Ni, Jinfeng Zhang, Youming Li, Ruijuan Front Microbiol Microbiology Strawberry gray mold caused by Botrytis cinerea is one of the most severe diseases in pre- and post-harvest periods. Although fungicides have been an effective way to control this disease, they can cause serious “3R” problems (Resistance, Resurgence and Residue). In this study, Streptomyces sp. sdu1201 isolated from the hindgut of the fungus-growing termite Odontotermes formosanus revealed significant antifungal activity against B. cinerea. Four compounds (1–4) were isolated from Streptomyces sp. sdu1201 and further identified as actinomycins by the HRMS and 1D NMR data. Among them, actinomycin D had the strongest inhibitory activity against B. cinerea with the EC(50) value of 7.65 μg mL(−1). The control effect of actinomycin D on strawberry gray mold was also tested on fruits and leaves in vitro, and its control efficiency on leaves was 78.77% at 3 d. Moreover, actinomycin D can also inhibit the polarized growth of germ tubes of B. cinerea. Therefore, Streptomyces sp. sdu1201 and actinomycin D have great potential to gray mold as biocontrol agents. Frontiers Media S.A. 2022-11-04 /pmc/articles/PMC9674021/ /pubmed/36406410 http://dx.doi.org/10.3389/fmicb.2022.1051730 Text en Copyright © 2022 Yong, Li, Gong, Yu, Zhao, Duan, Ren, Li, Fu, Ni, Zhang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yong, Daojing Li, Yue Gong, Kai Yu, Yingying Zhao, Shuai Duan, Qiong Ren, Cailing Li, Aiying Fu, Jun Ni, Jinfeng Zhang, Youming Li, Ruijuan Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D |
title | Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D |
title_full | Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D |
title_fullStr | Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D |
title_full_unstemmed | Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D |
title_short | Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D |
title_sort | biocontrol of strawberry gray mold caused by botrytis cinerea with the termite associated streptomyces sp. sdu1201 and actinomycin d |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9674021/ https://www.ncbi.nlm.nih.gov/pubmed/36406410 http://dx.doi.org/10.3389/fmicb.2022.1051730 |
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