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Impact of pulmonary African trypanosomes on the immunology and function of the lung

Approximately 20% of sleeping sickness patients exhibit respiratory complications, however, with a largely unknown role of the parasite. Here we show that tsetse fly-transmitted Trypanosoma brucei parasites rapidly and permanently colonize the lungs and occupy the extravascular spaces surrounding th...

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Autores principales: Mabille, Dorien, Dirkx, Laura, Thys, Sofie, Vermeersch, Marjorie, Montenye, Daniel, Govaerts, Matthias, Hendrickx, Sarah, Takac, Peter, Van Weyenbergh, Johan, Pintelon, Isabel, Delputte, Peter, Maes, Louis, Pérez-Morga, David, Timmermans, Jean-Pierre, Caljon, Guy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9674601/
https://www.ncbi.nlm.nih.gov/pubmed/36400767
http://dx.doi.org/10.1038/s41467-022-34757-w
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author Mabille, Dorien
Dirkx, Laura
Thys, Sofie
Vermeersch, Marjorie
Montenye, Daniel
Govaerts, Matthias
Hendrickx, Sarah
Takac, Peter
Van Weyenbergh, Johan
Pintelon, Isabel
Delputte, Peter
Maes, Louis
Pérez-Morga, David
Timmermans, Jean-Pierre
Caljon, Guy
author_facet Mabille, Dorien
Dirkx, Laura
Thys, Sofie
Vermeersch, Marjorie
Montenye, Daniel
Govaerts, Matthias
Hendrickx, Sarah
Takac, Peter
Van Weyenbergh, Johan
Pintelon, Isabel
Delputte, Peter
Maes, Louis
Pérez-Morga, David
Timmermans, Jean-Pierre
Caljon, Guy
author_sort Mabille, Dorien
collection PubMed
description Approximately 20% of sleeping sickness patients exhibit respiratory complications, however, with a largely unknown role of the parasite. Here we show that tsetse fly-transmitted Trypanosoma brucei parasites rapidly and permanently colonize the lungs and occupy the extravascular spaces surrounding the blood vessels of the alveoli and bronchi. They are present as nests of multiplying parasites exhibiting close interactions with collagen and active secretion of extracellular vesicles. The local immune response shows a substantial increase of monocytes, macrophages, dendritic cells and γδ and activated αβ T cells and a later influx of neutrophils. Interestingly, parasite presence results in a significant reduction of B cells, eosinophils and natural killer cells. T. brucei infected mice show no infection-associated pulmonary dysfunction, mirroring the limited pulmonary clinical complications during sleeping sickness. However, the substantial reduction of the various immune cells may render individuals more susceptible to opportunistic infections, as evident by a co-infection experiment with respiratory syncytial virus. Collectively, these observations provide insights into a largely overlooked target organ, and may trigger new diagnostic and supportive therapeutic approaches for sleeping sickness.
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spelling pubmed-96746012022-11-20 Impact of pulmonary African trypanosomes on the immunology and function of the lung Mabille, Dorien Dirkx, Laura Thys, Sofie Vermeersch, Marjorie Montenye, Daniel Govaerts, Matthias Hendrickx, Sarah Takac, Peter Van Weyenbergh, Johan Pintelon, Isabel Delputte, Peter Maes, Louis Pérez-Morga, David Timmermans, Jean-Pierre Caljon, Guy Nat Commun Article Approximately 20% of sleeping sickness patients exhibit respiratory complications, however, with a largely unknown role of the parasite. Here we show that tsetse fly-transmitted Trypanosoma brucei parasites rapidly and permanently colonize the lungs and occupy the extravascular spaces surrounding the blood vessels of the alveoli and bronchi. They are present as nests of multiplying parasites exhibiting close interactions with collagen and active secretion of extracellular vesicles. The local immune response shows a substantial increase of monocytes, macrophages, dendritic cells and γδ and activated αβ T cells and a later influx of neutrophils. Interestingly, parasite presence results in a significant reduction of B cells, eosinophils and natural killer cells. T. brucei infected mice show no infection-associated pulmonary dysfunction, mirroring the limited pulmonary clinical complications during sleeping sickness. However, the substantial reduction of the various immune cells may render individuals more susceptible to opportunistic infections, as evident by a co-infection experiment with respiratory syncytial virus. Collectively, these observations provide insights into a largely overlooked target organ, and may trigger new diagnostic and supportive therapeutic approaches for sleeping sickness. Nature Publishing Group UK 2022-11-18 /pmc/articles/PMC9674601/ /pubmed/36400767 http://dx.doi.org/10.1038/s41467-022-34757-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mabille, Dorien
Dirkx, Laura
Thys, Sofie
Vermeersch, Marjorie
Montenye, Daniel
Govaerts, Matthias
Hendrickx, Sarah
Takac, Peter
Van Weyenbergh, Johan
Pintelon, Isabel
Delputte, Peter
Maes, Louis
Pérez-Morga, David
Timmermans, Jean-Pierre
Caljon, Guy
Impact of pulmonary African trypanosomes on the immunology and function of the lung
title Impact of pulmonary African trypanosomes on the immunology and function of the lung
title_full Impact of pulmonary African trypanosomes on the immunology and function of the lung
title_fullStr Impact of pulmonary African trypanosomes on the immunology and function of the lung
title_full_unstemmed Impact of pulmonary African trypanosomes on the immunology and function of the lung
title_short Impact of pulmonary African trypanosomes on the immunology and function of the lung
title_sort impact of pulmonary african trypanosomes on the immunology and function of the lung
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9674601/
https://www.ncbi.nlm.nih.gov/pubmed/36400767
http://dx.doi.org/10.1038/s41467-022-34757-w
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