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Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia
Sparassis latifolia is a valuable edible mushroom, growing on fresh pine wood sawdust substrate. However, the mechanistic bases are poorly understood. The gene expression profiles of S. latifolia were analyzed from submerged cultures with fresh pine wood sawdust substrate for different time (0 h, 1 ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9674834/ https://www.ncbi.nlm.nih.gov/pubmed/36400936 http://dx.doi.org/10.1038/s41598-022-24171-z |
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author | Yang, Chi Ma, Lu Xiao, Donglai Liu, Xiaoyu Jiang, Xiaoling Lin, Yanquan |
author_facet | Yang, Chi Ma, Lu Xiao, Donglai Liu, Xiaoyu Jiang, Xiaoling Lin, Yanquan |
author_sort | Yang, Chi |
collection | PubMed |
description | Sparassis latifolia is a valuable edible mushroom, growing on fresh pine wood sawdust substrate. However, the mechanistic bases are poorly understood. The gene expression profiles of S. latifolia were analyzed from submerged cultures with fresh pine wood sawdust substrate for different time (0 h, 1 h, 6 h, 1 day, 5 days, and 10 days, respectively). The total number of differentially expressed genes (DEGs) identified under pine sawdust inducing was 2,659 compared to 0 h (CK). And 1,073, 520, 385, 424, and 257 DEGs were identified at the five time points, respectively. There were 34 genes in common at all inoculated time points, including FAD/NAD(P)-binding domain-containing protein, glucose methanol choline (GMC) oxidoreductase, flavin-containing monooxygenase, and taurine catabolism dioxygenase. Weighted gene co-expression analysis (WGCNA) was then used to compare the molecular characteristics among the groups and identified that the blue module had the highest correlation with the time induced by pine wood sawdust. There were 102 DEGs out of 125 genes in the blue model, which were most enriched in nitronate monooxygenase activity, dioxygenase activity, and oxidation–reduction process GO terms (p < 0.05), and peroxisome in KEGG pathway. This may provide clues into mechanisms that S. latifolia can grow on fresh pine wood sawdust substrate. |
format | Online Article Text |
id | pubmed-9674834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96748342022-11-20 Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia Yang, Chi Ma, Lu Xiao, Donglai Liu, Xiaoyu Jiang, Xiaoling Lin, Yanquan Sci Rep Article Sparassis latifolia is a valuable edible mushroom, growing on fresh pine wood sawdust substrate. However, the mechanistic bases are poorly understood. The gene expression profiles of S. latifolia were analyzed from submerged cultures with fresh pine wood sawdust substrate for different time (0 h, 1 h, 6 h, 1 day, 5 days, and 10 days, respectively). The total number of differentially expressed genes (DEGs) identified under pine sawdust inducing was 2,659 compared to 0 h (CK). And 1,073, 520, 385, 424, and 257 DEGs were identified at the five time points, respectively. There were 34 genes in common at all inoculated time points, including FAD/NAD(P)-binding domain-containing protein, glucose methanol choline (GMC) oxidoreductase, flavin-containing monooxygenase, and taurine catabolism dioxygenase. Weighted gene co-expression analysis (WGCNA) was then used to compare the molecular characteristics among the groups and identified that the blue module had the highest correlation with the time induced by pine wood sawdust. There were 102 DEGs out of 125 genes in the blue model, which were most enriched in nitronate monooxygenase activity, dioxygenase activity, and oxidation–reduction process GO terms (p < 0.05), and peroxisome in KEGG pathway. This may provide clues into mechanisms that S. latifolia can grow on fresh pine wood sawdust substrate. Nature Publishing Group UK 2022-11-18 /pmc/articles/PMC9674834/ /pubmed/36400936 http://dx.doi.org/10.1038/s41598-022-24171-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Chi Ma, Lu Xiao, Donglai Liu, Xiaoyu Jiang, Xiaoling Lin, Yanquan Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia |
title | Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia |
title_full | Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia |
title_fullStr | Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia |
title_full_unstemmed | Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia |
title_short | Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia |
title_sort | comparative transcriptomics reveals unique pine wood decay strategies in the sparassis latifolia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9674834/ https://www.ncbi.nlm.nih.gov/pubmed/36400936 http://dx.doi.org/10.1038/s41598-022-24171-z |
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