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Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd

BACKGROUND: Wax gourd [Benincasa hispida (Thunb) Cogn. (2n = 2x = 24)] is an economically important vegetable crop of genus Benincasa in the Cucurbitaceae family. Fruit is the main consumption organ of wax gourd. The mature fruit cuticular wax (MFCW) is an important trait in breeding programs, which...

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Autores principales: Yan, Jinqiang, Chen, Feng, Sun, Piaoyun, Liu, Wenrui, Xie, Dasen, Qian, Yulei, Jiang, Biao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675113/
https://www.ncbi.nlm.nih.gov/pubmed/36401157
http://dx.doi.org/10.1186/s12870-022-03931-z
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author Yan, Jinqiang
Chen, Feng
Sun, Piaoyun
Liu, Wenrui
Xie, Dasen
Qian, Yulei
Jiang, Biao
author_facet Yan, Jinqiang
Chen, Feng
Sun, Piaoyun
Liu, Wenrui
Xie, Dasen
Qian, Yulei
Jiang, Biao
author_sort Yan, Jinqiang
collection PubMed
description BACKGROUND: Wax gourd [Benincasa hispida (Thunb) Cogn. (2n = 2x = 24)] is an economically important vegetable crop of genus Benincasa in the Cucurbitaceae family. Fruit is the main consumption organ of wax gourd. The mature fruit cuticular wax (MFCW) is an important trait in breeding programs, which is also of evolutionary significance in wax gourd. However, the genetic architecture of this valuable trait remains unrevealed. RESULTS: In this study, genetic analysis revealed that the inheritance of MFCW was controlled by a single gene, with MFCW dominant over non-MFCW, and the gene was primarily named as BhWAX. Genome-wide association study (GWAS) highlighted a 1.1 Mb interval on chromosome 9 associated with MFCW in wax gourd germplasm resources. Traditional fine genetic mapping delimited BhWAX to a 0.5 Mb region containing 12 genes. Based on the gene annotation, expression analysis and co-segregation analysis, Bhi09G001428 that encodes a membrane bound O-acyltransferase (MBOAT) was proposed as the candidate gene for BhWAX. Moreover, it was demonstrated that the efficiency of a cleaved amplified polymorphic sequences (CAPS) marker in the determination of MFCW in wax gourd reached 80%. CONCLUSIONS: In closing, the study identified the candidate gene controlling MFCW and provided an efficient molecular marker for the trait in wax gourd for the first time, which will not only be beneficial for functional validation of the gene and marker-assisted breeding of wax gourd, but also lay a foundation for analysis of its evolutionary meaning among cucurbits. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03931-z.
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spelling pubmed-96751132022-11-20 Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd Yan, Jinqiang Chen, Feng Sun, Piaoyun Liu, Wenrui Xie, Dasen Qian, Yulei Jiang, Biao BMC Plant Biol Research Article BACKGROUND: Wax gourd [Benincasa hispida (Thunb) Cogn. (2n = 2x = 24)] is an economically important vegetable crop of genus Benincasa in the Cucurbitaceae family. Fruit is the main consumption organ of wax gourd. The mature fruit cuticular wax (MFCW) is an important trait in breeding programs, which is also of evolutionary significance in wax gourd. However, the genetic architecture of this valuable trait remains unrevealed. RESULTS: In this study, genetic analysis revealed that the inheritance of MFCW was controlled by a single gene, with MFCW dominant over non-MFCW, and the gene was primarily named as BhWAX. Genome-wide association study (GWAS) highlighted a 1.1 Mb interval on chromosome 9 associated with MFCW in wax gourd germplasm resources. Traditional fine genetic mapping delimited BhWAX to a 0.5 Mb region containing 12 genes. Based on the gene annotation, expression analysis and co-segregation analysis, Bhi09G001428 that encodes a membrane bound O-acyltransferase (MBOAT) was proposed as the candidate gene for BhWAX. Moreover, it was demonstrated that the efficiency of a cleaved amplified polymorphic sequences (CAPS) marker in the determination of MFCW in wax gourd reached 80%. CONCLUSIONS: In closing, the study identified the candidate gene controlling MFCW and provided an efficient molecular marker for the trait in wax gourd for the first time, which will not only be beneficial for functional validation of the gene and marker-assisted breeding of wax gourd, but also lay a foundation for analysis of its evolutionary meaning among cucurbits. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03931-z. BioMed Central 2022-11-19 /pmc/articles/PMC9675113/ /pubmed/36401157 http://dx.doi.org/10.1186/s12870-022-03931-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Yan, Jinqiang
Chen, Feng
Sun, Piaoyun
Liu, Wenrui
Xie, Dasen
Qian, Yulei
Jiang, Biao
Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd
title Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd
title_full Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd
title_fullStr Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd
title_full_unstemmed Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd
title_short Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd
title_sort genome-wide association study and genetic mapping of bhwax conferring mature fruit cuticular wax in wax gourd
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675113/
https://www.ncbi.nlm.nih.gov/pubmed/36401157
http://dx.doi.org/10.1186/s12870-022-03931-z
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