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Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation
Diabetes mellitus (DM) is highly comorbid with severe dengue diseases; however, the underlying mechanisms are unclear. Patients with DM have a 1.61-fold increased risk of developing dengue hemorrhagic fever. In search of host factors involved in dengue virus (DENV) infection, we used high-glucose (H...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Clinical Investigation
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675471/ https://www.ncbi.nlm.nih.gov/pubmed/36125898 http://dx.doi.org/10.1172/jci.insight.142805 |
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author | Shen, Ting-Jing Chen, Chia-Ling Tsai, Tsung-Ting Jhan, Ming-Kai Bai, Chyi-Huey Yen, Yu-Chun Tsai, Ching-Wen Tseng, Po-Chun Yu, Chia-Yi Lin, Chiou-Feng |
author_facet | Shen, Ting-Jing Chen, Chia-Ling Tsai, Tsung-Ting Jhan, Ming-Kai Bai, Chyi-Huey Yen, Yu-Chun Tsai, Ching-Wen Tseng, Po-Chun Yu, Chia-Yi Lin, Chiou-Feng |
author_sort | Shen, Ting-Jing |
collection | PubMed |
description | Diabetes mellitus (DM) is highly comorbid with severe dengue diseases; however, the underlying mechanisms are unclear. Patients with DM have a 1.61-fold increased risk of developing dengue hemorrhagic fever. In search of host factors involved in dengue virus (DENV) infection, we used high-glucose (HG) treatment and showed that HG increased viral protein expression and virion release but had no effects on the early stages of viral infection. After HG stimulation, DENV–firefly luciferase–transfected assay and cellular replicon–based assay indicated increased viral translation, whereas using the glucose uptake inhibitor phloretin blocked this effect. HG treatment increased the translational factor poly(A)-binding protein (PABP) in a glucose transporter–associated, PI3K/AKT-regulated manner. Silencing PABP significantly decreased HG-prompted virion production. HG enhanced the formation of the PABP–eukaryotic translation initiation factor 4G complex, which is regulated by protein–disulfide isomerase. Hyperglycemia increased PABP expression, mortality rate, viral protein expression, and viral loads in streptozotocin-induced DM mice. Overall, hyperglycemic stress facilitates DENV infection by strengthening PABP-mediated viral translation. |
format | Online Article Text |
id | pubmed-9675471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Clinical Investigation |
record_format | MEDLINE/PubMed |
spelling | pubmed-96754712022-11-21 Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation Shen, Ting-Jing Chen, Chia-Ling Tsai, Tsung-Ting Jhan, Ming-Kai Bai, Chyi-Huey Yen, Yu-Chun Tsai, Ching-Wen Tseng, Po-Chun Yu, Chia-Yi Lin, Chiou-Feng JCI Insight Research Article Diabetes mellitus (DM) is highly comorbid with severe dengue diseases; however, the underlying mechanisms are unclear. Patients with DM have a 1.61-fold increased risk of developing dengue hemorrhagic fever. In search of host factors involved in dengue virus (DENV) infection, we used high-glucose (HG) treatment and showed that HG increased viral protein expression and virion release but had no effects on the early stages of viral infection. After HG stimulation, DENV–firefly luciferase–transfected assay and cellular replicon–based assay indicated increased viral translation, whereas using the glucose uptake inhibitor phloretin blocked this effect. HG treatment increased the translational factor poly(A)-binding protein (PABP) in a glucose transporter–associated, PI3K/AKT-regulated manner. Silencing PABP significantly decreased HG-prompted virion production. HG enhanced the formation of the PABP–eukaryotic translation initiation factor 4G complex, which is regulated by protein–disulfide isomerase. Hyperglycemia increased PABP expression, mortality rate, viral protein expression, and viral loads in streptozotocin-induced DM mice. Overall, hyperglycemic stress facilitates DENV infection by strengthening PABP-mediated viral translation. American Society for Clinical Investigation 2022-11-08 /pmc/articles/PMC9675471/ /pubmed/36125898 http://dx.doi.org/10.1172/jci.insight.142805 Text en © 2022 Shen et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Shen, Ting-Jing Chen, Chia-Ling Tsai, Tsung-Ting Jhan, Ming-Kai Bai, Chyi-Huey Yen, Yu-Chun Tsai, Ching-Wen Tseng, Po-Chun Yu, Chia-Yi Lin, Chiou-Feng Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation |
title | Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation |
title_full | Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation |
title_fullStr | Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation |
title_full_unstemmed | Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation |
title_short | Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein–mediated viral translation |
title_sort | hyperglycemia exacerbates dengue virus infection by facilitating poly(a)-binding protein–mediated viral translation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675471/ https://www.ncbi.nlm.nih.gov/pubmed/36125898 http://dx.doi.org/10.1172/jci.insight.142805 |
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