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Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age
Interferon-gamma release assays performance can be impaired by host-related, technical and environmental factors, but data in young children are limited. We performed a cross-sectional study of children < 5 years-of-age at risk of tuberculosis (TB), using QuantiFERON-TB Gold In-Tube (QFT-GIT) ass...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675832/ https://www.ncbi.nlm.nih.gov/pubmed/36402803 http://dx.doi.org/10.1038/s41598-022-24433-w |
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author | Velasco-Arnaiz, Eneritz Batllori, Marta Monsonís, Manuel Valls, Anna Ríos-Barnes, María Simó-Nebot, Sílvia Gamell, Anna Fortuny, Clàudia Tebruegge, Marc Noguera-Julian, Antoni |
author_facet | Velasco-Arnaiz, Eneritz Batllori, Marta Monsonís, Manuel Valls, Anna Ríos-Barnes, María Simó-Nebot, Sílvia Gamell, Anna Fortuny, Clàudia Tebruegge, Marc Noguera-Julian, Antoni |
author_sort | Velasco-Arnaiz, Eneritz |
collection | PubMed |
description | Interferon-gamma release assays performance can be impaired by host-related, technical and environmental factors, but data in young children are limited. We performed a cross-sectional study of children < 5 years-of-age at risk of tuberculosis (TB), using QuantiFERON-TB Gold In-Tube (QFT-GIT) assays. The impact of the following was evaluated: (i) host-related [age; hematological parameters; erythrocyte sedimentation rate (ESR); C-reactive protein (CRP); and tobacco smoke exposure (TSE) based on serum cotinine concentrations], (ii) technical (pre-analytical delay) and (iii) environmental factors (annual season; monthly temperatures). Of 204 children, 35 (17.2%) were diagnosed with latent TB infection or TB disease. QFT-GIT results were indeterminate in 14 (6.9%) patients. In multivariate analysis, younger age and higher ESR were associated with lower positive control responses (beta: 0.247, p = 0.002 and − 0.204, p = 0.007, respectively), and increasing age was associated with lower rates of indeterminate QFT-GIT results [OR (95% CI) 0.948 (0.903–0.996) per month, p = 0.035]. In children with positive QFT-GIT results, average monthly temperatures correlated with antigen responses (r = 0.453, p = 0.020); also, antigen responses were lower in winter than in other seasons (p = 0.027). Serum cotinine concentrations determined in a subgroup of patients (n = 41) indicated TSE in 36 (88%), positive control responses being lower in children with TSE (p = 0.034). In children < 5 years-of-age, young age, elevated ESR, temperature, annual season and TSE can affect the performance of QFT-GIT assays. |
format | Online Article Text |
id | pubmed-9675832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96758322022-11-21 Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age Velasco-Arnaiz, Eneritz Batllori, Marta Monsonís, Manuel Valls, Anna Ríos-Barnes, María Simó-Nebot, Sílvia Gamell, Anna Fortuny, Clàudia Tebruegge, Marc Noguera-Julian, Antoni Sci Rep Article Interferon-gamma release assays performance can be impaired by host-related, technical and environmental factors, but data in young children are limited. We performed a cross-sectional study of children < 5 years-of-age at risk of tuberculosis (TB), using QuantiFERON-TB Gold In-Tube (QFT-GIT) assays. The impact of the following was evaluated: (i) host-related [age; hematological parameters; erythrocyte sedimentation rate (ESR); C-reactive protein (CRP); and tobacco smoke exposure (TSE) based on serum cotinine concentrations], (ii) technical (pre-analytical delay) and (iii) environmental factors (annual season; monthly temperatures). Of 204 children, 35 (17.2%) were diagnosed with latent TB infection or TB disease. QFT-GIT results were indeterminate in 14 (6.9%) patients. In multivariate analysis, younger age and higher ESR were associated with lower positive control responses (beta: 0.247, p = 0.002 and − 0.204, p = 0.007, respectively), and increasing age was associated with lower rates of indeterminate QFT-GIT results [OR (95% CI) 0.948 (0.903–0.996) per month, p = 0.035]. In children with positive QFT-GIT results, average monthly temperatures correlated with antigen responses (r = 0.453, p = 0.020); also, antigen responses were lower in winter than in other seasons (p = 0.027). Serum cotinine concentrations determined in a subgroup of patients (n = 41) indicated TSE in 36 (88%), positive control responses being lower in children with TSE (p = 0.034). In children < 5 years-of-age, young age, elevated ESR, temperature, annual season and TSE can affect the performance of QFT-GIT assays. Nature Publishing Group UK 2022-11-19 /pmc/articles/PMC9675832/ /pubmed/36402803 http://dx.doi.org/10.1038/s41598-022-24433-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Velasco-Arnaiz, Eneritz Batllori, Marta Monsonís, Manuel Valls, Anna Ríos-Barnes, María Simó-Nebot, Sílvia Gamell, Anna Fortuny, Clàudia Tebruegge, Marc Noguera-Julian, Antoni Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
title | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
title_full | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
title_fullStr | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
title_full_unstemmed | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
title_short | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
title_sort | host, technical, and environmental factors affecting quantiferon-tb gold in-tube performance in children below 5 years of age |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675832/ https://www.ncbi.nlm.nih.gov/pubmed/36402803 http://dx.doi.org/10.1038/s41598-022-24433-w |
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