Cargando…

Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s

Diphtheria is a potentially fatal respiratory disease caused by toxigenic forms of the Gram-positive bacterium Corynebacterium diphtheriae . Despite the availability of treatments (antitoxin and antimicrobials) and effective vaccines, the disease still occurs sporadically in low-income countries and...

Descripción completa

Detalles Bibliográficos
Autores principales: Nguyen Thi Nguyen, To, Parry, Christopher M., Campbell, James I., Vinh, Phat Voong, Kneen, Rachel, Baker, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676054/
https://www.ncbi.nlm.nih.gov/pubmed/36259695
http://dx.doi.org/10.1099/mgen.0.000861
_version_ 1784833507774693376
author Nguyen Thi Nguyen, To
Parry, Christopher M.
Campbell, James I.
Vinh, Phat Voong
Kneen, Rachel
Baker, Stephen
author_facet Nguyen Thi Nguyen, To
Parry, Christopher M.
Campbell, James I.
Vinh, Phat Voong
Kneen, Rachel
Baker, Stephen
author_sort Nguyen Thi Nguyen, To
collection PubMed
description Diphtheria is a potentially fatal respiratory disease caused by toxigenic forms of the Gram-positive bacterium Corynebacterium diphtheriae . Despite the availability of treatments (antitoxin and antimicrobials) and effective vaccines, the disease still occurs sporadically in low-income countries and in higher income where use of diphtheria vaccine is inconsistent. Diphtheria was highly endemic in Vietnam in the 1990s; here, we aimed to provide some historical context to the circulation of erythromycin resistant organisms in Vietnam during this period. After recovering 54 C . diphtheriae isolated from clinical cases of diphtheria in Ho Chi Minh City between 1992 and 1998 we conducted whole genome sequencing and analysis. Our data outlined substantial genetic diversity among the isolates, illustrated by seven distinct Sequence Types (STs), but punctuated by the sustained circulation of ST67 and ST209. With the exception of one isolate, all sequences contained the tox gene, which was classically located on a corynebacteriophage. All erythromycin resistant isolates, accounting for 13 % of organisms in this study, harboured a novel 18 kb erm(X)-carrying plasmid, which exhibited limited sequence homology to previously described resistance plasmids in C. diphtheriae . Our study provides historic context for the circulation of antimicrobial resistant C. diphtheriae in Vietnam; these data provide a framework for the current trajectory in global antimicrobial resistance trends.
format Online
Article
Text
id pubmed-9676054
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-96760542022-11-21 Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s Nguyen Thi Nguyen, To Parry, Christopher M. Campbell, James I. Vinh, Phat Voong Kneen, Rachel Baker, Stephen Microb Genom Research Articles Diphtheria is a potentially fatal respiratory disease caused by toxigenic forms of the Gram-positive bacterium Corynebacterium diphtheriae . Despite the availability of treatments (antitoxin and antimicrobials) and effective vaccines, the disease still occurs sporadically in low-income countries and in higher income where use of diphtheria vaccine is inconsistent. Diphtheria was highly endemic in Vietnam in the 1990s; here, we aimed to provide some historical context to the circulation of erythromycin resistant organisms in Vietnam during this period. After recovering 54 C . diphtheriae isolated from clinical cases of diphtheria in Ho Chi Minh City between 1992 and 1998 we conducted whole genome sequencing and analysis. Our data outlined substantial genetic diversity among the isolates, illustrated by seven distinct Sequence Types (STs), but punctuated by the sustained circulation of ST67 and ST209. With the exception of one isolate, all sequences contained the tox gene, which was classically located on a corynebacteriophage. All erythromycin resistant isolates, accounting for 13 % of organisms in this study, harboured a novel 18 kb erm(X)-carrying plasmid, which exhibited limited sequence homology to previously described resistance plasmids in C. diphtheriae . Our study provides historic context for the circulation of antimicrobial resistant C. diphtheriae in Vietnam; these data provide a framework for the current trajectory in global antimicrobial resistance trends. Microbiology Society 2022-10-19 /pmc/articles/PMC9676054/ /pubmed/36259695 http://dx.doi.org/10.1099/mgen.0.000861 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Research Articles
Nguyen Thi Nguyen, To
Parry, Christopher M.
Campbell, James I.
Vinh, Phat Voong
Kneen, Rachel
Baker, Stephen
Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s
title Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s
title_full Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s
title_fullStr Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s
title_full_unstemmed Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s
title_short Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s
title_sort endemic erythromycin resistant corynebacterium diphtheriae in vietnam in the 1990s
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676054/
https://www.ncbi.nlm.nih.gov/pubmed/36259695
http://dx.doi.org/10.1099/mgen.0.000861
work_keys_str_mv AT nguyenthinguyento endemicerythromycinresistantcorynebacteriumdiphtheriaeinvietnaminthe1990s
AT parrychristopherm endemicerythromycinresistantcorynebacteriumdiphtheriaeinvietnaminthe1990s
AT campbelljamesi endemicerythromycinresistantcorynebacteriumdiphtheriaeinvietnaminthe1990s
AT vinhphatvoong endemicerythromycinresistantcorynebacteriumdiphtheriaeinvietnaminthe1990s
AT kneenrachel endemicerythromycinresistantcorynebacteriumdiphtheriaeinvietnaminthe1990s
AT bakerstephen endemicerythromycinresistantcorynebacteriumdiphtheriaeinvietnaminthe1990s