Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models

The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to character...

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Detalles Bibliográficos
Autores principales: Bartikoski, Bárbara Jonson, Longo, Larisse, Gabriel de Souza, Valessa Emanoele, Salvati, Fernando, Uribe-Cruz, Carolina, Lenz, Guido, Xavier, Ricardo Machado, Álvares-da-Silva, Mário Reis, Visioli, Fernanda, Filippi-Chiela, Eduardo Cremonese
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676206/
https://www.ncbi.nlm.nih.gov/pubmed/36595941
http://dx.doi.org/10.1016/j.xpro.2022.101855
Descripción
Sumario:The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to characterize physiopathological conditions related to muscle tissue. The main limitation of MusMA is the need for high-quality tissue slides and images and control samples to set up the analyses.

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