Modified dietary fiber from cassava pulp affects the cecal microbial population, short-chain fatty acid, and ammonia production in broiler chickens

The objective of this study was to investigate the effects of modified dietary fiber from cassava pulp (M-DFCP) supplementation in broiler diets on cecal microbial populations, short-chain fatty acids (SCFAs), ammonia production, and immune responses. A total of 336, one-day-old male broiler chicks (Ross 308) were distributed over 4 dietary treatments in 7 replicate pens (n = 12 chicks) using a completely randomized design. Chicks were fed the control diet and 3 levels of M-DFCP (0.5, 1.0, and 1.5%) for an experimental duration of 42 d. The M-DFCP contained total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF), cello-oligosaccharides (COS), and xylo-oligosaccharides (XOS) of approximately 280.70, 22.20, 258.50, 23.93, and 157.55 g/kg, respectively. The 1.0 and 1.5% M-DFCP supplementation diets showed positive effects on stimulating the growth of Lactobacillus spp. and Bifidobacterium spp., enhancing SCFAs (acetic, propionic, butyric acid, and branched SCFAs) and lactic acid concentrations during growing periods. Broilers fed 1.0 and 1.5% M-DFCP also exhibited a significant increase in caecal Lactobacillus spp. and lactic acid concentrations during the finisher period as well. In addition, M-DFCP also reduced cecal digesta and excreta ammonia production in broilers over both periods (0–21 and 22–42 d of age). However, M-DFCP did not exhibit any effect on total serum immunoglobulin (Ig) or lysozyme activity. In conclusion, this study shows that M-DFCP can be used as a dietary fiber source in broiler diets, with a recommended level of approximately 1.0%.