Cargando…

Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) is a usual oral cancer. Therefore, it's essential to identify targets for its early diagnosis and therapy. This research aimed to explore the roles of human β-defensin-3 (hBD-3) and nuclear factor-kappa B (NF-κB) p65 in the pathogenesis and progression of OSC...

Descripción completa

Detalles Bibliográficos
Autores principales: Du, Yongxiu, Yang, Yanlan, Zhang, Wenbo, Yang, Chenxi, Xu, Pu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676516/
https://www.ncbi.nlm.nih.gov/pubmed/36403504
http://dx.doi.org/10.1016/j.tranon.2022.101582
_version_ 1784833614607810560
author Du, Yongxiu
Yang, Yanlan
Zhang, Wenbo
Yang, Chenxi
Xu, Pu
author_facet Du, Yongxiu
Yang, Yanlan
Zhang, Wenbo
Yang, Chenxi
Xu, Pu
author_sort Du, Yongxiu
collection PubMed
description Oral squamous cell carcinoma (OSCC) is a usual oral cancer. Therefore, it's essential to identify targets for its early diagnosis and therapy. This research aimed to explore the roles of human β-defensin-3 (hBD-3) and nuclear factor-kappa B (NF-κB) p65 in the pathogenesis and progression of OSCC. The connection between NF-κB p65 and the carcinogenesis of oral cancer was analyzed by immunohistochemical staining. The relative expressions of hBD-3 and NF-κB p65 in OSCC cells were evaluated by qRT-PCR and Western blot. Afterward, hBD-3 was knocked down, and NF-κB p65 was overexpressed. The cell viability and invasion were tested via CCK-8 and Transwell experiment, and the expression of hBD-3, NF-κB p65, and its downstream molecules was evaluated by Western blot. The expression of NF-κB p65 was increased with the aggravation of the oral submucosal fibrosis. HBD-3 and NF-κB p65 were high-expressed in OSCC cells. The viability and invasion abilities of OSCC cells that knocked down hBD-3 were markedly decreased, while they were restored by the overexpression of NF-κB p65. The expressions of NF-κB p65 and c-myc were diminished while IκB and p21 were raised with the knockdown of hBD-3. After overexpression of NF-κB p65, the expression of hBD-3 and IκB did not change markedly, while c-myc was increased and p21 was decreased dramatically. HBD-3 and NF-κB p65 facilitate the proliferation and invasion of OSCC cells, and hBD-3 may promote this process by governing the expression of NF-κB p65 and its downstream c-myc and p21.
format Online
Article
Text
id pubmed-9676516
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Neoplasia Press
record_format MEDLINE/PubMed
spelling pubmed-96765162022-11-25 Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma Du, Yongxiu Yang, Yanlan Zhang, Wenbo Yang, Chenxi Xu, Pu Transl Oncol Commentary Oral squamous cell carcinoma (OSCC) is a usual oral cancer. Therefore, it's essential to identify targets for its early diagnosis and therapy. This research aimed to explore the roles of human β-defensin-3 (hBD-3) and nuclear factor-kappa B (NF-κB) p65 in the pathogenesis and progression of OSCC. The connection between NF-κB p65 and the carcinogenesis of oral cancer was analyzed by immunohistochemical staining. The relative expressions of hBD-3 and NF-κB p65 in OSCC cells were evaluated by qRT-PCR and Western blot. Afterward, hBD-3 was knocked down, and NF-κB p65 was overexpressed. The cell viability and invasion were tested via CCK-8 and Transwell experiment, and the expression of hBD-3, NF-κB p65, and its downstream molecules was evaluated by Western blot. The expression of NF-κB p65 was increased with the aggravation of the oral submucosal fibrosis. HBD-3 and NF-κB p65 were high-expressed in OSCC cells. The viability and invasion abilities of OSCC cells that knocked down hBD-3 were markedly decreased, while they were restored by the overexpression of NF-κB p65. The expressions of NF-κB p65 and c-myc were diminished while IκB and p21 were raised with the knockdown of hBD-3. After overexpression of NF-κB p65, the expression of hBD-3 and IκB did not change markedly, while c-myc was increased and p21 was decreased dramatically. HBD-3 and NF-κB p65 facilitate the proliferation and invasion of OSCC cells, and hBD-3 may promote this process by governing the expression of NF-κB p65 and its downstream c-myc and p21. Neoplasia Press 2022-11-18 /pmc/articles/PMC9676516/ /pubmed/36403504 http://dx.doi.org/10.1016/j.tranon.2022.101582 Text en © 2022 Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Commentary
Du, Yongxiu
Yang, Yanlan
Zhang, Wenbo
Yang, Chenxi
Xu, Pu
Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma
title Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma
title_full Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma
title_fullStr Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma
title_full_unstemmed Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma
title_short Human β-defensin-3 and nuclear factor-kappa B p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma
title_sort human β-defensin-3 and nuclear factor-kappa b p65 synergistically promote the cell proliferation and invasion of oral squamous cell carcinoma
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676516/
https://www.ncbi.nlm.nih.gov/pubmed/36403504
http://dx.doi.org/10.1016/j.tranon.2022.101582
work_keys_str_mv AT duyongxiu humanbdefensin3andnuclearfactorkappabp65synergisticallypromotethecellproliferationandinvasionoforalsquamouscellcarcinoma
AT yangyanlan humanbdefensin3andnuclearfactorkappabp65synergisticallypromotethecellproliferationandinvasionoforalsquamouscellcarcinoma
AT zhangwenbo humanbdefensin3andnuclearfactorkappabp65synergisticallypromotethecellproliferationandinvasionoforalsquamouscellcarcinoma
AT yangchenxi humanbdefensin3andnuclearfactorkappabp65synergisticallypromotethecellproliferationandinvasionoforalsquamouscellcarcinoma
AT xupu humanbdefensin3andnuclearfactorkappabp65synergisticallypromotethecellproliferationandinvasionoforalsquamouscellcarcinoma