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Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’

Lilium is a popular cut flower that is highly favored by consumers due to its snowy white color and strong fragrance, which originates from the release of monoterpenes. However, the underlying molecular mechanism of monoterpene synthesis remains poorly understood. In this study, the content of three...

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Autores principales: Yang, Yun-Yao, Ma, Bo, Li, Ying-Ying, Han, Ming-Zheng, Wu, Jing, Zhou, Xiao-Feng, Tian, Ji, Wang, Wen-He, Leng, Ping-Sheng, Hu, Zeng-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677127/
https://www.ncbi.nlm.nih.gov/pubmed/36420028
http://dx.doi.org/10.3389/fpls.2022.1021576
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author Yang, Yun-Yao
Ma, Bo
Li, Ying-Ying
Han, Ming-Zheng
Wu, Jing
Zhou, Xiao-Feng
Tian, Ji
Wang, Wen-He
Leng, Ping-Sheng
Hu, Zeng-Hui
author_facet Yang, Yun-Yao
Ma, Bo
Li, Ying-Ying
Han, Ming-Zheng
Wu, Jing
Zhou, Xiao-Feng
Tian, Ji
Wang, Wen-He
Leng, Ping-Sheng
Hu, Zeng-Hui
author_sort Yang, Yun-Yao
collection PubMed
description Lilium is a popular cut flower that is highly favored by consumers due to its snowy white color and strong fragrance, which originates from the release of monoterpenes. However, the underlying molecular mechanism of monoterpene synthesis remains poorly understood. In this study, the content of three main monoterpenes (linalool, ocimene, and myrcene) was examined in Lilium ‘Siberia’, and RNA sequencing of the 11 stages of flower development was conducted. The biosynthesis of the three monoterpenes increased with flower development, reaching their peak levels at the full flowering stage. Transcriptome data revealed 257,140 unigenes, with an average size of 794 bp, from which 43,934 differentially expressed genes were identified and enriched in the KEGG pathways partly involved in plant hormone signal transduction and monoterpenoid biosynthesis. Furthermore, the essential factor LiMYB305 was identified by WGCNA after the release of the flower fragrance. The transient silencing of LiMYB305 in petals using VIGS technology showed that the mRNA expression levels of LiLiS, LiOcS, and LiMyS were significantly downregulated and that the release of linalool, ocimene, and myrcene had decreased significantly. Y1H, LUC, and EMSA experiments revealed that LiMYB305 directly bound and activated the LiOcS promoter to increase the synthesis of monoterpenes. Taken together, these results provide insight into the molecular mechanism of monoterpene synthesis and provide valuable information to investigate the formation of the flower fragrance in Lilium.
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spelling pubmed-96771272022-11-22 Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’ Yang, Yun-Yao Ma, Bo Li, Ying-Ying Han, Ming-Zheng Wu, Jing Zhou, Xiao-Feng Tian, Ji Wang, Wen-He Leng, Ping-Sheng Hu, Zeng-Hui Front Plant Sci Plant Science Lilium is a popular cut flower that is highly favored by consumers due to its snowy white color and strong fragrance, which originates from the release of monoterpenes. However, the underlying molecular mechanism of monoterpene synthesis remains poorly understood. In this study, the content of three main monoterpenes (linalool, ocimene, and myrcene) was examined in Lilium ‘Siberia’, and RNA sequencing of the 11 stages of flower development was conducted. The biosynthesis of the three monoterpenes increased with flower development, reaching their peak levels at the full flowering stage. Transcriptome data revealed 257,140 unigenes, with an average size of 794 bp, from which 43,934 differentially expressed genes were identified and enriched in the KEGG pathways partly involved in plant hormone signal transduction and monoterpenoid biosynthesis. Furthermore, the essential factor LiMYB305 was identified by WGCNA after the release of the flower fragrance. The transient silencing of LiMYB305 in petals using VIGS technology showed that the mRNA expression levels of LiLiS, LiOcS, and LiMyS were significantly downregulated and that the release of linalool, ocimene, and myrcene had decreased significantly. Y1H, LUC, and EMSA experiments revealed that LiMYB305 directly bound and activated the LiOcS promoter to increase the synthesis of monoterpenes. Taken together, these results provide insight into the molecular mechanism of monoterpene synthesis and provide valuable information to investigate the formation of the flower fragrance in Lilium. Frontiers Media S.A. 2022-11-07 /pmc/articles/PMC9677127/ /pubmed/36420028 http://dx.doi.org/10.3389/fpls.2022.1021576 Text en Copyright © 2022 Yang, Ma, Li, Han, Wu, Zhou, Tian, Wang, Leng and Hu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Yun-Yao
Ma, Bo
Li, Ying-Ying
Han, Ming-Zheng
Wu, Jing
Zhou, Xiao-Feng
Tian, Ji
Wang, Wen-He
Leng, Ping-Sheng
Hu, Zeng-Hui
Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’
title Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’
title_full Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’
title_fullStr Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’
title_full_unstemmed Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’
title_short Transcriptome analysis identifies key gene LiMYB305 involved in monoterpene biosynthesis in Lilium ‘Siberia’
title_sort transcriptome analysis identifies key gene limyb305 involved in monoterpene biosynthesis in lilium ‘siberia’
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677127/
https://www.ncbi.nlm.nih.gov/pubmed/36420028
http://dx.doi.org/10.3389/fpls.2022.1021576
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