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Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China

BACKGROUND: Tigecycline is regarded as a last‐resort antimicrobial agent against multidrug resistance (MDR). However, resistance‐nodulation‐division efflux pump resistance genes, such as tmexCD3‐toprJ3, that confer resistance to tigecycline have emerged. OBJECTIVES: This study aimed to characterise...

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Autores principales: Yang, Jie, Zeng, Ziyue, Hu, Jufang, Liu, Zhihong, Gu, Jinrong, Chen, Xiaojun, Sun, Zhiliang, Li, Jiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677350/
https://www.ncbi.nlm.nih.gov/pubmed/36130135
http://dx.doi.org/10.1002/vms3.950
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author Yang, Jie
Zeng, Ziyue
Hu, Jufang
Liu, Zhihong
Gu, Jinrong
Chen, Xiaojun
Sun, Zhiliang
Li, Jiyun
author_facet Yang, Jie
Zeng, Ziyue
Hu, Jufang
Liu, Zhihong
Gu, Jinrong
Chen, Xiaojun
Sun, Zhiliang
Li, Jiyun
author_sort Yang, Jie
collection PubMed
description BACKGROUND: Tigecycline is regarded as a last‐resort antimicrobial agent against multidrug resistance (MDR). However, resistance‐nodulation‐division efflux pump resistance genes, such as tmexCD3‐toprJ3, that confer resistance to tigecycline have emerged. OBJECTIVES: This study aimed to characterise the tmexCD3‐toprJ3 gene cluster in Pseudomonas juntendi and Proteus terrae isolated from a pig farm. METHODS: Samples were obtained from a Chinese piggery and included 92 pig faecal samples, 56 wastewater samples, 23 drinking water samples, 28 sow vagina samples and nine sow uterus swabs. The presence of tmexCD3‐toprJ3 was detected using a polymerase chain reaction assay, and the antimicrobial susceptibility profile of the tmexCD3‐toprJ3‐positive isolates was determined using the broth dilution method. Genomic locations were identified using whole‐genome sequencing and bioinformatics. RESULTS: We identified two tmexCD3‐toprJ3‐positive isolates, and both isolates were highly resistant to antibiotics such as tigecycline. In addition, we identified several mobile elements (ISPa7, ISCfr1, ISVsa3) and insertion sequences (TnAs2, TnAs3) in tmexCD3‐toprJ3‐positive isolates that could increase the potential for the spread of MDR. CONCLUSIONS: To the best of our knowledge, this study is the first to report the detection of tmexCD3‐toprJ3 in P. juntendi and P. terrae isolated from a pig farm.
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spelling pubmed-96773502022-11-22 Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China Yang, Jie Zeng, Ziyue Hu, Jufang Liu, Zhihong Gu, Jinrong Chen, Xiaojun Sun, Zhiliang Li, Jiyun Vet Med Sci PIGS BACKGROUND: Tigecycline is regarded as a last‐resort antimicrobial agent against multidrug resistance (MDR). However, resistance‐nodulation‐division efflux pump resistance genes, such as tmexCD3‐toprJ3, that confer resistance to tigecycline have emerged. OBJECTIVES: This study aimed to characterise the tmexCD3‐toprJ3 gene cluster in Pseudomonas juntendi and Proteus terrae isolated from a pig farm. METHODS: Samples were obtained from a Chinese piggery and included 92 pig faecal samples, 56 wastewater samples, 23 drinking water samples, 28 sow vagina samples and nine sow uterus swabs. The presence of tmexCD3‐toprJ3 was detected using a polymerase chain reaction assay, and the antimicrobial susceptibility profile of the tmexCD3‐toprJ3‐positive isolates was determined using the broth dilution method. Genomic locations were identified using whole‐genome sequencing and bioinformatics. RESULTS: We identified two tmexCD3‐toprJ3‐positive isolates, and both isolates were highly resistant to antibiotics such as tigecycline. In addition, we identified several mobile elements (ISPa7, ISCfr1, ISVsa3) and insertion sequences (TnAs2, TnAs3) in tmexCD3‐toprJ3‐positive isolates that could increase the potential for the spread of MDR. CONCLUSIONS: To the best of our knowledge, this study is the first to report the detection of tmexCD3‐toprJ3 in P. juntendi and P. terrae isolated from a pig farm. John Wiley and Sons Inc. 2022-09-21 /pmc/articles/PMC9677350/ /pubmed/36130135 http://dx.doi.org/10.1002/vms3.950 Text en © 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle PIGS
Yang, Jie
Zeng, Ziyue
Hu, Jufang
Liu, Zhihong
Gu, Jinrong
Chen, Xiaojun
Sun, Zhiliang
Li, Jiyun
Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China
title Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China
title_full Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China
title_fullStr Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China
title_full_unstemmed Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China
title_short Emergence of the resistance‐nodulation‐division efflux pump tmexCD3‐toprJ3 gene confers resistance to tigecycline in Pseudomonas juntendi and Proteus terrae isolated from a pig farm in China
title_sort emergence of the resistance‐nodulation‐division efflux pump tmexcd3‐toprj3 gene confers resistance to tigecycline in pseudomonas juntendi and proteus terrae isolated from a pig farm in china
topic PIGS
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677350/
https://www.ncbi.nlm.nih.gov/pubmed/36130135
http://dx.doi.org/10.1002/vms3.950
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