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Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)

BACKGROUND: Enrofloxacin (ENR) is a kind of quinolone antibiotic that is most widely used antimicrobials in veterinary practice, and possesses both a broad spectrum antimicrobial activity against a range of bacteria and adverse effects towards plants and animals. OBJECTIVES: This study was conducted...

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Detalles Bibliográficos
Autores principales: Zhang, Fan, Wang, Runping, Huang, Jianzhen, Zhang, Haixin, Wang, Long, Zhong, Zhiwei, Ruan, Jiming, Liu, Huazhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677365/
https://www.ncbi.nlm.nih.gov/pubmed/36037402
http://dx.doi.org/10.1002/vms3.918
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author Zhang, Fan
Wang, Runping
Huang, Jianzhen
Zhang, Haixin
Wang, Long
Zhong, Zhiwei
Ruan, Jiming
Liu, Huazhong
author_facet Zhang, Fan
Wang, Runping
Huang, Jianzhen
Zhang, Haixin
Wang, Long
Zhong, Zhiwei
Ruan, Jiming
Liu, Huazhong
author_sort Zhang, Fan
collection PubMed
description BACKGROUND: Enrofloxacin (ENR) is a kind of quinolone antibiotic that is most widely used antimicrobials in veterinary practice, and possesses both a broad spectrum antimicrobial activity against a range of bacteria and adverse effects towards plants and animals. OBJECTIVES: This study was conducted to explore the permeability of blood–brain barrier (BBB) to ENR and brain injury based on crucian carp orally administrated with high dose of ENR. METHODS: Juvenile Pengze crucian carp were treated with half lethal dose (LD(50)) or safe dose (SD(50)) of ENR. BBB permeability was determined by evaluating ENR contents detected by HPLC and evens blue contents estimated by confocal laser scanning microscope. Brain damage was evaluated by measuring protein and mRNA contents of related molecules with western blotting and qPCR. RESULTS: Data indicated that ENR destroyed BBB structure of crucian carp and enhanced permeability of the biological barrier, resulting in more ENR crossed BBB and induced brain damage of crucian carp. CONCLUSIONS: This data indicated that ENR can induce brain damage of crucian carp through destroying BBB structure and enhancing permeability.
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spelling pubmed-96773652022-11-22 Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze) Zhang, Fan Wang, Runping Huang, Jianzhen Zhang, Haixin Wang, Long Zhong, Zhiwei Ruan, Jiming Liu, Huazhong Vet Med Sci FISH BACKGROUND: Enrofloxacin (ENR) is a kind of quinolone antibiotic that is most widely used antimicrobials in veterinary practice, and possesses both a broad spectrum antimicrobial activity against a range of bacteria and adverse effects towards plants and animals. OBJECTIVES: This study was conducted to explore the permeability of blood–brain barrier (BBB) to ENR and brain injury based on crucian carp orally administrated with high dose of ENR. METHODS: Juvenile Pengze crucian carp were treated with half lethal dose (LD(50)) or safe dose (SD(50)) of ENR. BBB permeability was determined by evaluating ENR contents detected by HPLC and evens blue contents estimated by confocal laser scanning microscope. Brain damage was evaluated by measuring protein and mRNA contents of related molecules with western blotting and qPCR. RESULTS: Data indicated that ENR destroyed BBB structure of crucian carp and enhanced permeability of the biological barrier, resulting in more ENR crossed BBB and induced brain damage of crucian carp. CONCLUSIONS: This data indicated that ENR can induce brain damage of crucian carp through destroying BBB structure and enhancing permeability. John Wiley and Sons Inc. 2022-08-29 /pmc/articles/PMC9677365/ /pubmed/36037402 http://dx.doi.org/10.1002/vms3.918 Text en © 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle FISH
Zhang, Fan
Wang, Runping
Huang, Jianzhen
Zhang, Haixin
Wang, Long
Zhong, Zhiwei
Ruan, Jiming
Liu, Huazhong
Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)
title Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)
title_full Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)
title_fullStr Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)
title_full_unstemmed Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)
title_short Blood–brain barrier permeability to enrofloxacin in Pengze crucian carp (Carassius auratus var. Pengze)
title_sort blood–brain barrier permeability to enrofloxacin in pengze crucian carp (carassius auratus var. pengze)
topic FISH
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677365/
https://www.ncbi.nlm.nih.gov/pubmed/36037402
http://dx.doi.org/10.1002/vms3.918
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