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Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity

BACKGROUND: Qing Chang oral liquid (QOL) is a veterinary drug, which mainly composed of Artemisiae annuae herba, Dichroae radix, Agrimonia pilosa and Sanguisorbae radix. OBJECTIVES: This study aims to explore the effect of Qing Chang Oral Liquid (QOL) on the treatment effect of artificially infected...

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Autores principales: Zhi‐qiang, Yan, Qian‐lin, Chen, Li‐zhi, Fu, Wen‐gui, Fu, Hua, Zheng, Hong‐mei, Tang, Shao‐qin, Zhai, Chun‐lin, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677391/
https://www.ncbi.nlm.nih.gov/pubmed/36112763
http://dx.doi.org/10.1002/vms3.922
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author Zhi‐qiang, Yan
Qian‐lin, Chen
Li‐zhi, Fu
Wen‐gui, Fu
Hua, Zheng
Hong‐mei, Tang
Shao‐qin, Zhai
Chun‐lin, Chen
author_facet Zhi‐qiang, Yan
Qian‐lin, Chen
Li‐zhi, Fu
Wen‐gui, Fu
Hua, Zheng
Hong‐mei, Tang
Shao‐qin, Zhai
Chun‐lin, Chen
author_sort Zhi‐qiang, Yan
collection PubMed
description BACKGROUND: Qing Chang oral liquid (QOL) is a veterinary drug, which mainly composed of Artemisiae annuae herba, Dichroae radix, Agrimonia pilosa and Sanguisorbae radix. OBJECTIVES: This study aims to explore the effect of Qing Chang Oral Liquid (QOL) on the treatment effect of artificially infected chicken coccidiosis and to the cellular immunity. METHODS: Healthy Roman chickens were randomly divided into five groups: blank group, model group, QOL high‐, medium‐ and low‐dose groups. All the groups were orally administered with 1 × 10(4) sporulated oocysts (except the blank group). After 5 days of oral administration, the high‐, medium‐ and low‐dose groups of QOL were added to the drinking water at 2.4, 1.8 and 1.2 ml/kg, respectively. The blank and model groups were fed normally, and this experiment lasted for 7 days. The clinical signs were observed, and the relative weight gain, survival rate, cecum lesion score and oocyst value were measured to evaluate the effect of QOL. Meanwhile, the peripheral blood T‐lymphocyte subsets and cecal IL‐2, IL‐17, IFN‐γ mRNA expression were detected by flow cytometry and fluorescent quantitative PCR. RESULTS: The chickens in the model group were in poor mental state, gathered together, had loose stools or bloody stools and had less food intake and less exercise. The chicken mental state improved, the food intake and drinking water increased, and the faeces are normal in the high‐, medium‐ and low‐dose groups, especially in the high‐dose group, the anti‐coccidial indexes were up to 173.08. No significant differences were observed (p > 0.05) in the peripheral blood CD3(+), CD3(+)CD4(+) between the experimental groups. Compared with the blank group, there were different degrees of increase in each dose drug group of the cecal IFN‐γ, IL‐2 and IL‐17 mRNA expression, but the high‐dose group was significantly reduced compared with the model group (p < 0.01), but there was no significant difference (p > 0.05). CONCLUSIONS: This study suggests that QOL has positive anti‐coccidial effect but has no obvious effect on the cellular immunity.
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spelling pubmed-96773912022-11-22 Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity Zhi‐qiang, Yan Qian‐lin, Chen Li‐zhi, Fu Wen‐gui, Fu Hua, Zheng Hong‐mei, Tang Shao‐qin, Zhai Chun‐lin, Chen Vet Med Sci POULTRY BACKGROUND: Qing Chang oral liquid (QOL) is a veterinary drug, which mainly composed of Artemisiae annuae herba, Dichroae radix, Agrimonia pilosa and Sanguisorbae radix. OBJECTIVES: This study aims to explore the effect of Qing Chang Oral Liquid (QOL) on the treatment effect of artificially infected chicken coccidiosis and to the cellular immunity. METHODS: Healthy Roman chickens were randomly divided into five groups: blank group, model group, QOL high‐, medium‐ and low‐dose groups. All the groups were orally administered with 1 × 10(4) sporulated oocysts (except the blank group). After 5 days of oral administration, the high‐, medium‐ and low‐dose groups of QOL were added to the drinking water at 2.4, 1.8 and 1.2 ml/kg, respectively. The blank and model groups were fed normally, and this experiment lasted for 7 days. The clinical signs were observed, and the relative weight gain, survival rate, cecum lesion score and oocyst value were measured to evaluate the effect of QOL. Meanwhile, the peripheral blood T‐lymphocyte subsets and cecal IL‐2, IL‐17, IFN‐γ mRNA expression were detected by flow cytometry and fluorescent quantitative PCR. RESULTS: The chickens in the model group were in poor mental state, gathered together, had loose stools or bloody stools and had less food intake and less exercise. The chicken mental state improved, the food intake and drinking water increased, and the faeces are normal in the high‐, medium‐ and low‐dose groups, especially in the high‐dose group, the anti‐coccidial indexes were up to 173.08. No significant differences were observed (p > 0.05) in the peripheral blood CD3(+), CD3(+)CD4(+) between the experimental groups. Compared with the blank group, there were different degrees of increase in each dose drug group of the cecal IFN‐γ, IL‐2 and IL‐17 mRNA expression, but the high‐dose group was significantly reduced compared with the model group (p < 0.01), but there was no significant difference (p > 0.05). CONCLUSIONS: This study suggests that QOL has positive anti‐coccidial effect but has no obvious effect on the cellular immunity. John Wiley and Sons Inc. 2022-09-16 /pmc/articles/PMC9677391/ /pubmed/36112763 http://dx.doi.org/10.1002/vms3.922 Text en © 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle POULTRY
Zhi‐qiang, Yan
Qian‐lin, Chen
Li‐zhi, Fu
Wen‐gui, Fu
Hua, Zheng
Hong‐mei, Tang
Shao‐qin, Zhai
Chun‐lin, Chen
Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity
title Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity
title_full Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity
title_fullStr Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity
title_full_unstemmed Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity
title_short Effect of Qing Chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity
title_sort effect of qing chang oral liquid on the treatment of artificially infected chicken coccidiosis and the cellular immunity
topic POULTRY
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677391/
https://www.ncbi.nlm.nih.gov/pubmed/36112763
http://dx.doi.org/10.1002/vms3.922
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