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Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia
BACKGROUND: One of the consequences of the presentation of reproductive failures in sows is the economic losses in production because it alters the estimated values of the volume of production, decreasing the productivity of the farm. Porcine circovirosis by porcine circovirus 2 (PCV2) has been asso...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677394/ https://www.ncbi.nlm.nih.gov/pubmed/36137294 http://dx.doi.org/10.1002/vms3.949 |
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author | Uribe‐García, Heinner F. Suarez‐Mesa, Rafael A. Rondón‐Barragán, Iang S. |
author_facet | Uribe‐García, Heinner F. Suarez‐Mesa, Rafael A. Rondón‐Barragán, Iang S. |
author_sort | Uribe‐García, Heinner F. |
collection | PubMed |
description | BACKGROUND: One of the consequences of the presentation of reproductive failures in sows is the economic losses in production because it alters the estimated values of the volume of production, decreasing the productivity of the farm. Porcine circovirosis by porcine circovirus 2 (PCV2) has been associated with reproductive disorders, and porcine parvovirus (PVP) is one of the pathological agents most related to the presentation of reproductive failure in pigs. In Colombia, there are reports of the presence of PCV2 through molecular techniques, and PVP through serum tests; however, in the department of Tolima, the prevalence of these two viruses is unknown. OBJECTIVE: In this study, the aim was to establish a report of the prevalence of viruses in five municipalities of the department of Tolima‐Colombia. METHODS: Blood samples from 150 breeding sows of five municipalities in Tolima, Colombia, were obtained. Quantitative polymerase chain reaction (qPCR) was used to detect the PCV2 and PVP virus in the blood samples followed by PCR and sequencing of 16 PCR products of the amplification of the cap gene of PCV2. A phylogenetic tree was constructed to identify the genotype of the PCV2 virus. RESULTS: The presence of PCV2d in sows was detected in 135 samples (90%), as well as the identification of PVP in 2.6% of the samples. In addition, the phylogenetic analysis showed that 16 isolates were the PCV2d2 genotype. CONCLUSION: PCV2d and PVP were found to coinfect the females, and the identification of variability in regions in the predicted amino acid sequence of the PCV2 capsid may be associated with virus pathogenicity. |
format | Online Article Text |
id | pubmed-9677394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96773942022-11-22 Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia Uribe‐García, Heinner F. Suarez‐Mesa, Rafael A. Rondón‐Barragán, Iang S. Vet Med Sci PIGS BACKGROUND: One of the consequences of the presentation of reproductive failures in sows is the economic losses in production because it alters the estimated values of the volume of production, decreasing the productivity of the farm. Porcine circovirosis by porcine circovirus 2 (PCV2) has been associated with reproductive disorders, and porcine parvovirus (PVP) is one of the pathological agents most related to the presentation of reproductive failure in pigs. In Colombia, there are reports of the presence of PCV2 through molecular techniques, and PVP through serum tests; however, in the department of Tolima, the prevalence of these two viruses is unknown. OBJECTIVE: In this study, the aim was to establish a report of the prevalence of viruses in five municipalities of the department of Tolima‐Colombia. METHODS: Blood samples from 150 breeding sows of five municipalities in Tolima, Colombia, were obtained. Quantitative polymerase chain reaction (qPCR) was used to detect the PCV2 and PVP virus in the blood samples followed by PCR and sequencing of 16 PCR products of the amplification of the cap gene of PCV2. A phylogenetic tree was constructed to identify the genotype of the PCV2 virus. RESULTS: The presence of PCV2d in sows was detected in 135 samples (90%), as well as the identification of PVP in 2.6% of the samples. In addition, the phylogenetic analysis showed that 16 isolates were the PCV2d2 genotype. CONCLUSION: PCV2d and PVP were found to coinfect the females, and the identification of variability in regions in the predicted amino acid sequence of the PCV2 capsid may be associated with virus pathogenicity. John Wiley and Sons Inc. 2022-09-22 /pmc/articles/PMC9677394/ /pubmed/36137294 http://dx.doi.org/10.1002/vms3.949 Text en © 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | PIGS Uribe‐García, Heinner F. Suarez‐Mesa, Rafael A. Rondón‐Barragán, Iang S. Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia |
title | Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia |
title_full | Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia |
title_fullStr | Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia |
title_full_unstemmed | Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia |
title_short | Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia |
title_sort | survey of porcine circovirus type 2 and parvovirus in swine breeding herds of colombia |
topic | PIGS |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677394/ https://www.ncbi.nlm.nih.gov/pubmed/36137294 http://dx.doi.org/10.1002/vms3.949 |
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