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Gene expression profile of placentomes and clinical parameters in the cows with retained placenta
BACKGROUND: Retained placenta (RP) is a prevalent disorder in cattle with many health-related and economic costs for the farm owners. Its etiology has not been clarified yet and there is no definite therapy for this disorder. In this study we conducted RNA-seq, hematologic and histologic experiments...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677913/ https://www.ncbi.nlm.nih.gov/pubmed/36411408 http://dx.doi.org/10.1186/s12864-022-08989-5 |
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author | Moradi, Mehdi Zhandi, Mahdi Sharafi, Mohsen Akbari, Arvand Atrabi, Mohammad Jafari Totonchi, Mehdi |
author_facet | Moradi, Mehdi Zhandi, Mahdi Sharafi, Mohsen Akbari, Arvand Atrabi, Mohammad Jafari Totonchi, Mehdi |
author_sort | Moradi, Mehdi |
collection | PubMed |
description | BACKGROUND: Retained placenta (RP) is a prevalent disorder in cattle with many health-related and economic costs for the farm owners. Its etiology has not been clarified yet and there is no definite therapy for this disorder. In this study we conducted RNA-seq, hematologic and histologic experiments to survey the causes of RP development. METHODS: Blood samples were collected from 4 RP and 3 healthy cows during periparturtion period for hematological assessments followed by placentome sampling within 30 min after parturition. Cows were grouped as RP and control in case the placenta was retained or otherwise expelled, respectively. Total RNA was extracted from placentome samples followed by RNA-sequencing. RESULTS: We showed 240 differentially expressed genes (DEGs) between the RP and control groups. Enrichment analyzes indicated immune system and lipid metabolism as prominent over- and under-represented pathways in RP cows, respectively. Hormonal assessments showed that estradiol-17β (E2) was lower and cortisol tended to be higher in RP cows compared to controls at the day of parturition. Furthermore, histologic experiment showed that villi-crypt junctions remain tighter in RP cows compared to controls and the crypts layer seemed thicker in the placentome of RP cows. Complete blood cell (CBC) parameters were not significantly different between the two groups. CONCLUSION: Overall, DEGs derived from expression profiling and these genes contributed to enrichment of immune and lipid metabolism pathways. We suggested that E2 could be involved in development of RP and the concentrations of P4 and CBC counts periparturition might not be a determining factor. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08989-5. |
format | Online Article Text |
id | pubmed-9677913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-96779132022-11-22 Gene expression profile of placentomes and clinical parameters in the cows with retained placenta Moradi, Mehdi Zhandi, Mahdi Sharafi, Mohsen Akbari, Arvand Atrabi, Mohammad Jafari Totonchi, Mehdi BMC Genomics Research BACKGROUND: Retained placenta (RP) is a prevalent disorder in cattle with many health-related and economic costs for the farm owners. Its etiology has not been clarified yet and there is no definite therapy for this disorder. In this study we conducted RNA-seq, hematologic and histologic experiments to survey the causes of RP development. METHODS: Blood samples were collected from 4 RP and 3 healthy cows during periparturtion period for hematological assessments followed by placentome sampling within 30 min after parturition. Cows were grouped as RP and control in case the placenta was retained or otherwise expelled, respectively. Total RNA was extracted from placentome samples followed by RNA-sequencing. RESULTS: We showed 240 differentially expressed genes (DEGs) between the RP and control groups. Enrichment analyzes indicated immune system and lipid metabolism as prominent over- and under-represented pathways in RP cows, respectively. Hormonal assessments showed that estradiol-17β (E2) was lower and cortisol tended to be higher in RP cows compared to controls at the day of parturition. Furthermore, histologic experiment showed that villi-crypt junctions remain tighter in RP cows compared to controls and the crypts layer seemed thicker in the placentome of RP cows. Complete blood cell (CBC) parameters were not significantly different between the two groups. CONCLUSION: Overall, DEGs derived from expression profiling and these genes contributed to enrichment of immune and lipid metabolism pathways. We suggested that E2 could be involved in development of RP and the concentrations of P4 and CBC counts periparturition might not be a determining factor. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08989-5. BioMed Central 2022-11-21 /pmc/articles/PMC9677913/ /pubmed/36411408 http://dx.doi.org/10.1186/s12864-022-08989-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Moradi, Mehdi Zhandi, Mahdi Sharafi, Mohsen Akbari, Arvand Atrabi, Mohammad Jafari Totonchi, Mehdi Gene expression profile of placentomes and clinical parameters in the cows with retained placenta |
title | Gene expression profile of placentomes and clinical parameters in the cows with retained placenta |
title_full | Gene expression profile of placentomes and clinical parameters in the cows with retained placenta |
title_fullStr | Gene expression profile of placentomes and clinical parameters in the cows with retained placenta |
title_full_unstemmed | Gene expression profile of placentomes and clinical parameters in the cows with retained placenta |
title_short | Gene expression profile of placentomes and clinical parameters in the cows with retained placenta |
title_sort | gene expression profile of placentomes and clinical parameters in the cows with retained placenta |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677913/ https://www.ncbi.nlm.nih.gov/pubmed/36411408 http://dx.doi.org/10.1186/s12864-022-08989-5 |
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