OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice

Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (P(OsTZF1):OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. Th...

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Autores principales: Ilyas, Muhammad, Hussain Shah, Safdar, Fujita, Yasunari, Maruyama, Kyonoshin, Nakashima, Kazuo, Yamaguchi-Shinozaki, Kazuko, Jan, Asad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677997/
https://www.ncbi.nlm.nih.gov/pubmed/36398733
http://dx.doi.org/10.1080/15592324.2022.2142725
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author Ilyas, Muhammad
Hussain Shah, Safdar
Fujita, Yasunari
Maruyama, Kyonoshin
Nakashima, Kazuo
Yamaguchi-Shinozaki, Kazuko
Jan, Asad
author_facet Ilyas, Muhammad
Hussain Shah, Safdar
Fujita, Yasunari
Maruyama, Kyonoshin
Nakashima, Kazuo
Yamaguchi-Shinozaki, Kazuko
Jan, Asad
author_sort Ilyas, Muhammad
collection PubMed
description Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (P(OsTZF1):OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. The P(OsTZF1):OsTZF1-OX produced normal leaves without any lesion mimic phenotype and exhibited normal seed setting. The P(OsTZF1):OsTZF1-OX plants showed significantly increased tolerance to salt and drought stresses and enhanced post stress recovery. Microarray analysis revealed a total of 846 genes up-regulated and 360 genes down-regulated in P(OsTZF1):OsTZF1-OX salt-treated plants. Microarray analysis of P(OsTZF1:)OsTZF1-OX plants showed the regulation of many abiotic stress tolerance genes. These results suggest that OsTZF1-OX under own promoter show abiotic stress tolerance and produces no pleiotropic effect on phenotype of transgenic rice plant.
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spelling pubmed-96779972022-11-22 OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice Ilyas, Muhammad Hussain Shah, Safdar Fujita, Yasunari Maruyama, Kyonoshin Nakashima, Kazuo Yamaguchi-Shinozaki, Kazuko Jan, Asad Plant Signal Behav Research Paper Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (P(OsTZF1):OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. The P(OsTZF1):OsTZF1-OX produced normal leaves without any lesion mimic phenotype and exhibited normal seed setting. The P(OsTZF1):OsTZF1-OX plants showed significantly increased tolerance to salt and drought stresses and enhanced post stress recovery. Microarray analysis revealed a total of 846 genes up-regulated and 360 genes down-regulated in P(OsTZF1):OsTZF1-OX salt-treated plants. Microarray analysis of P(OsTZF1:)OsTZF1-OX plants showed the regulation of many abiotic stress tolerance genes. These results suggest that OsTZF1-OX under own promoter show abiotic stress tolerance and produces no pleiotropic effect on phenotype of transgenic rice plant. Taylor & Francis 2022-11-18 /pmc/articles/PMC9677997/ /pubmed/36398733 http://dx.doi.org/10.1080/15592324.2022.2142725 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Ilyas, Muhammad
Hussain Shah, Safdar
Fujita, Yasunari
Maruyama, Kyonoshin
Nakashima, Kazuo
Yamaguchi-Shinozaki, Kazuko
Jan, Asad
OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice
title OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice
title_full OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice
title_fullStr OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice
title_full_unstemmed OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice
title_short OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice
title_sort ostzf1, a ccch-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677997/
https://www.ncbi.nlm.nih.gov/pubmed/36398733
http://dx.doi.org/10.1080/15592324.2022.2142725
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