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Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate

Literature has well-established the importance of 3-O-sulfation of neuronal cell surface glycan heparan sulfate (HS) to its interaction with herpes simplex virus type 1 glycoprotein D (gD). Previous investigations of gD to its viral receptors HVEM and nectin-1 also highlighted the conformational dyn...

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Autores principales: Gandy, Lauren A., Canning, Ashley J., Lou, Huan, Xia, Ke, He, Peng, Su, Guowei, Cairns, Tina, Liu, Jian, Zhang, Fuming, Linhardt, Robert J., Cohen, Gary, Wang, Chunyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678342/
https://www.ncbi.nlm.nih.gov/pubmed/36419932
http://dx.doi.org/10.3389/fmolb.2022.1043713
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author Gandy, Lauren A.
Canning, Ashley J.
Lou, Huan
Xia, Ke
He, Peng
Su, Guowei
Cairns, Tina
Liu, Jian
Zhang, Fuming
Linhardt, Robert J.
Cohen, Gary
Wang, Chunyu
author_facet Gandy, Lauren A.
Canning, Ashley J.
Lou, Huan
Xia, Ke
He, Peng
Su, Guowei
Cairns, Tina
Liu, Jian
Zhang, Fuming
Linhardt, Robert J.
Cohen, Gary
Wang, Chunyu
author_sort Gandy, Lauren A.
collection PubMed
description Literature has well-established the importance of 3-O-sulfation of neuronal cell surface glycan heparan sulfate (HS) to its interaction with herpes simplex virus type 1 glycoprotein D (gD). Previous investigations of gD to its viral receptors HVEM and nectin-1 also highlighted the conformational dynamics of gD’s N- and C-termini, necessary for viral membrane fusion. However, little is known on the structural interactions of gD with HS. Here, we present our findings on this interface from both the glycan and the protein perspective. We used C-terminal and N-terminal gD variants to probe the role of their respective regions in gD/HS binding. The N-terminal truncation mutants (with Δ1-22) demonstrate equivalent or stronger binding to heparin than their intact glycoproteins, indicating that the first 22 amino acids are disposable for heparin binding. Characterization of the conformational differences between C-terminal truncated mutants by sedimentation velocity analytical ultracentrifugation distinguished between the “open” and “closed” conformations of the glycoprotein D, highlighting the region’s modulation of receptor binding. From the glycan perspective, we investigated gD interacting with heparin, heparan sulfate, and other de-sulfated and chemically defined oligosaccharides using surface plasmon resonance and glycan microarray. The results show a strong preference of gD for 6-O-sulfate, with 2-O-sulfation becoming more important in the presence of 6-O-S. Additionally, 3-O-sulfation shifted the chain length preference of gD from longer chain to mid-chain length, reaffirming the sulfation site’s importance to the gD/HS interface. Our results shed new light on the molecular details of one of seven known protein-glycan interactions with 3-O-sulfated heparan sulfate.
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spelling pubmed-96783422022-11-22 Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate Gandy, Lauren A. Canning, Ashley J. Lou, Huan Xia, Ke He, Peng Su, Guowei Cairns, Tina Liu, Jian Zhang, Fuming Linhardt, Robert J. Cohen, Gary Wang, Chunyu Front Mol Biosci Molecular Biosciences Literature has well-established the importance of 3-O-sulfation of neuronal cell surface glycan heparan sulfate (HS) to its interaction with herpes simplex virus type 1 glycoprotein D (gD). Previous investigations of gD to its viral receptors HVEM and nectin-1 also highlighted the conformational dynamics of gD’s N- and C-termini, necessary for viral membrane fusion. However, little is known on the structural interactions of gD with HS. Here, we present our findings on this interface from both the glycan and the protein perspective. We used C-terminal and N-terminal gD variants to probe the role of their respective regions in gD/HS binding. The N-terminal truncation mutants (with Δ1-22) demonstrate equivalent or stronger binding to heparin than their intact glycoproteins, indicating that the first 22 amino acids are disposable for heparin binding. Characterization of the conformational differences between C-terminal truncated mutants by sedimentation velocity analytical ultracentrifugation distinguished between the “open” and “closed” conformations of the glycoprotein D, highlighting the region’s modulation of receptor binding. From the glycan perspective, we investigated gD interacting with heparin, heparan sulfate, and other de-sulfated and chemically defined oligosaccharides using surface plasmon resonance and glycan microarray. The results show a strong preference of gD for 6-O-sulfate, with 2-O-sulfation becoming more important in the presence of 6-O-S. Additionally, 3-O-sulfation shifted the chain length preference of gD from longer chain to mid-chain length, reaffirming the sulfation site’s importance to the gD/HS interface. Our results shed new light on the molecular details of one of seven known protein-glycan interactions with 3-O-sulfated heparan sulfate. Frontiers Media S.A. 2022-11-07 /pmc/articles/PMC9678342/ /pubmed/36419932 http://dx.doi.org/10.3389/fmolb.2022.1043713 Text en Copyright © 2022 Gandy, Canning, Lou, Xia, He, Su, Cairns, Liu, Zhang, Linhardt, Cohen and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Gandy, Lauren A.
Canning, Ashley J.
Lou, Huan
Xia, Ke
He, Peng
Su, Guowei
Cairns, Tina
Liu, Jian
Zhang, Fuming
Linhardt, Robert J.
Cohen, Gary
Wang, Chunyu
Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate
title Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate
title_full Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate
title_fullStr Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate
title_full_unstemmed Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate
title_short Molecular determinants of the interaction between HSV-1 glycoprotein D and heparan sulfate
title_sort molecular determinants of the interaction between hsv-1 glycoprotein d and heparan sulfate
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678342/
https://www.ncbi.nlm.nih.gov/pubmed/36419932
http://dx.doi.org/10.3389/fmolb.2022.1043713
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