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Drosophila mechanical nociceptors preferentially sense localized poking

Mechanical nociception is an evolutionarily conserved sensory process required for the survival of living organisms. Previous studies have revealed much about the neural circuits and sensory molecules in mechanical nociception, but the cellular mechanisms adopted by nociceptors in force detection re...

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Autores principales: Liu, Zhen, Wu, Meng-Hua, Wang, Qi-Xuan, Lin, Shao-Zhen, Feng, Xi-Qiao, Li, Bo, Liang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678358/
https://www.ncbi.nlm.nih.gov/pubmed/36200757
http://dx.doi.org/10.7554/eLife.76574
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author Liu, Zhen
Wu, Meng-Hua
Wang, Qi-Xuan
Lin, Shao-Zhen
Feng, Xi-Qiao
Li, Bo
Liang, Xin
author_facet Liu, Zhen
Wu, Meng-Hua
Wang, Qi-Xuan
Lin, Shao-Zhen
Feng, Xi-Qiao
Li, Bo
Liang, Xin
author_sort Liu, Zhen
collection PubMed
description Mechanical nociception is an evolutionarily conserved sensory process required for the survival of living organisms. Previous studies have revealed much about the neural circuits and sensory molecules in mechanical nociception, but the cellular mechanisms adopted by nociceptors in force detection remain elusive. To address this issue, we study the mechanosensation of a fly larval nociceptor (class IV da neurons, c4da) using a customized mechanical device. We find that c4da are sensitive to mN-scale forces and make uniform responses to the forces applied at different dendritic regions. Moreover, c4da showed a greater sensitivity to localized forces, consistent with them being able to detect the poking of sharp objects, such as wasp ovipositor. Further analysis reveals that high morphological complexity, mechanosensitivity to lateral tension and possibly also active signal propagation in dendrites contribute to the sensory features of c4da. In particular, we discover that Piezo and Ppk1/Ppk26, two key mechanosensory molecules, make differential but additive contributions to the mechanosensitivity of c4da. In all, our results provide updates into understanding how c4da process mechanical signals at the cellular level and reveal the contributions of key molecules.
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spelling pubmed-96783582022-11-22 Drosophila mechanical nociceptors preferentially sense localized poking Liu, Zhen Wu, Meng-Hua Wang, Qi-Xuan Lin, Shao-Zhen Feng, Xi-Qiao Li, Bo Liang, Xin eLife Neuroscience Mechanical nociception is an evolutionarily conserved sensory process required for the survival of living organisms. Previous studies have revealed much about the neural circuits and sensory molecules in mechanical nociception, but the cellular mechanisms adopted by nociceptors in force detection remain elusive. To address this issue, we study the mechanosensation of a fly larval nociceptor (class IV da neurons, c4da) using a customized mechanical device. We find that c4da are sensitive to mN-scale forces and make uniform responses to the forces applied at different dendritic regions. Moreover, c4da showed a greater sensitivity to localized forces, consistent with them being able to detect the poking of sharp objects, such as wasp ovipositor. Further analysis reveals that high morphological complexity, mechanosensitivity to lateral tension and possibly also active signal propagation in dendrites contribute to the sensory features of c4da. In particular, we discover that Piezo and Ppk1/Ppk26, two key mechanosensory molecules, make differential but additive contributions to the mechanosensitivity of c4da. In all, our results provide updates into understanding how c4da process mechanical signals at the cellular level and reveal the contributions of key molecules. eLife Sciences Publications, Ltd 2022-10-06 /pmc/articles/PMC9678358/ /pubmed/36200757 http://dx.doi.org/10.7554/eLife.76574 Text en © 2022, Liu, Wu et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Liu, Zhen
Wu, Meng-Hua
Wang, Qi-Xuan
Lin, Shao-Zhen
Feng, Xi-Qiao
Li, Bo
Liang, Xin
Drosophila mechanical nociceptors preferentially sense localized poking
title Drosophila mechanical nociceptors preferentially sense localized poking
title_full Drosophila mechanical nociceptors preferentially sense localized poking
title_fullStr Drosophila mechanical nociceptors preferentially sense localized poking
title_full_unstemmed Drosophila mechanical nociceptors preferentially sense localized poking
title_short Drosophila mechanical nociceptors preferentially sense localized poking
title_sort drosophila mechanical nociceptors preferentially sense localized poking
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678358/
https://www.ncbi.nlm.nih.gov/pubmed/36200757
http://dx.doi.org/10.7554/eLife.76574
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