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Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders

Polyvinyl chloride (PVC) is widely used in industrial applications, such as construction and clothing, owing to its chemical, physical, and environmental resistance. Owing to the previous characteristics, PVC is the third most consumed plastic worldwide and, consequently, an increasing waste accumul...

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Autores principales: Rodríguez-Fonseca, Maria Fernanda, Ruiz-Balaguera, Sonia, Valero, Manuel Fernando, Sánchez-Suárez, Jeysson, Coy-Barrera, Ericsson, Díaz, Luis Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678452/
https://www.ncbi.nlm.nih.gov/pubmed/36419778
http://dx.doi.org/10.1155/2022/6420003
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author Rodríguez-Fonseca, Maria Fernanda
Ruiz-Balaguera, Sonia
Valero, Manuel Fernando
Sánchez-Suárez, Jeysson
Coy-Barrera, Ericsson
Díaz, Luis Eduardo
author_facet Rodríguez-Fonseca, Maria Fernanda
Ruiz-Balaguera, Sonia
Valero, Manuel Fernando
Sánchez-Suárez, Jeysson
Coy-Barrera, Ericsson
Díaz, Luis Eduardo
author_sort Rodríguez-Fonseca, Maria Fernanda
collection PubMed
description Polyvinyl chloride (PVC) is widely used in industrial applications, such as construction and clothing, owing to its chemical, physical, and environmental resistance. Owing to the previous characteristics, PVC is the third most consumed plastic worldwide and, consequently, an increasing waste accumulation-related problem. The current study evaluated an in-house collection of 61 Actinobacteria strains for PVC resin biodegradation. Weight loss percentage was measured after the completion of incubation. Thermo-gravimetric analysis was subsequently performed using the PVC incubated with the three strains exhibiting the highest weight loss. GC-MS and ionic exchange chromatography analyses were also performed using the culture media supernatant of these three strains. After incubation, 14 strains had a PVC weight loss percentage higher than 50% in ISP-2 broth. These 14 strains were identified as Streptomyces strains. Strains 208, 250, and 290 showed the highest weight loss percentages (57.6–61.5% range). The thermal stability of PVC after bacterial exposure using these three strains was evaluated, and a modification of the representative degradation stages of nonincubated PVC was observed. Additionally, GC-MS analysis revealed the presence of aromatic compounds in the inoculated culture media, and ionic exchange chromatography showed chloride release in the supernatant. A mathematical relation between culture conditions and PVC weight loss was also found for strains 208 and 290, showing an accuracy up to 97.99%. These results highlight the potential of the freshwater-derived Streptomyces strains as candidates for the PVC biodegradation strategy and constitute the first approach to a waste management control scale-up process.
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spelling pubmed-96784522022-11-22 Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders Rodríguez-Fonseca, Maria Fernanda Ruiz-Balaguera, Sonia Valero, Manuel Fernando Sánchez-Suárez, Jeysson Coy-Barrera, Ericsson Díaz, Luis Eduardo ScientificWorldJournal Research Article Polyvinyl chloride (PVC) is widely used in industrial applications, such as construction and clothing, owing to its chemical, physical, and environmental resistance. Owing to the previous characteristics, PVC is the third most consumed plastic worldwide and, consequently, an increasing waste accumulation-related problem. The current study evaluated an in-house collection of 61 Actinobacteria strains for PVC resin biodegradation. Weight loss percentage was measured after the completion of incubation. Thermo-gravimetric analysis was subsequently performed using the PVC incubated with the three strains exhibiting the highest weight loss. GC-MS and ionic exchange chromatography analyses were also performed using the culture media supernatant of these three strains. After incubation, 14 strains had a PVC weight loss percentage higher than 50% in ISP-2 broth. These 14 strains were identified as Streptomyces strains. Strains 208, 250, and 290 showed the highest weight loss percentages (57.6–61.5% range). The thermal stability of PVC after bacterial exposure using these three strains was evaluated, and a modification of the representative degradation stages of nonincubated PVC was observed. Additionally, GC-MS analysis revealed the presence of aromatic compounds in the inoculated culture media, and ionic exchange chromatography showed chloride release in the supernatant. A mathematical relation between culture conditions and PVC weight loss was also found for strains 208 and 290, showing an accuracy up to 97.99%. These results highlight the potential of the freshwater-derived Streptomyces strains as candidates for the PVC biodegradation strategy and constitute the first approach to a waste management control scale-up process. Hindawi 2022-11-14 /pmc/articles/PMC9678452/ /pubmed/36419778 http://dx.doi.org/10.1155/2022/6420003 Text en Copyright © 2022 Maria Fernanda Rodríguez-Fonseca et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rodríguez-Fonseca, Maria Fernanda
Ruiz-Balaguera, Sonia
Valero, Manuel Fernando
Sánchez-Suárez, Jeysson
Coy-Barrera, Ericsson
Díaz, Luis Eduardo
Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_full Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_fullStr Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_full_unstemmed Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_short Freshwater-Derived Streptomyces: Prospective Polyvinyl Chloride (PVC) Biodegraders
title_sort freshwater-derived streptomyces: prospective polyvinyl chloride (pvc) biodegraders
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678452/
https://www.ncbi.nlm.nih.gov/pubmed/36419778
http://dx.doi.org/10.1155/2022/6420003
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