Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain

Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the...

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Detalles Bibliográficos
Autores principales: Sharma, Nikhil, Sen, Ganes C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678759/
https://www.ncbi.nlm.nih.gov/pubmed/36595897
http://dx.doi.org/10.1016/j.xpro.2022.101836
Descripción
Sumario:Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and Western blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).(1)

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