Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain

Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the...

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Detalles Bibliográficos
Autores principales: Sharma, Nikhil, Sen, Ganes C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678759/
https://www.ncbi.nlm.nih.gov/pubmed/36595897
http://dx.doi.org/10.1016/j.xpro.2022.101836
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author Sharma, Nikhil
Sen, Ganes C.
author_facet Sharma, Nikhil
Sen, Ganes C.
author_sort Sharma, Nikhil
collection PubMed
description Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and Western blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).(1)
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spelling pubmed-96787592022-11-23 Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain Sharma, Nikhil Sen, Ganes C. STAR Protoc Protocol Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and Western blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs. For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).(1) Elsevier 2022-11-18 /pmc/articles/PMC9678759/ /pubmed/36595897 http://dx.doi.org/10.1016/j.xpro.2022.101836 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sharma, Nikhil
Sen, Ganes C.
Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain
title Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain
title_full Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain
title_fullStr Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain
title_full_unstemmed Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain
title_short Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain
title_sort intracranial delivery of antisense locked nucleic acids for silencing micrornas in the mouse brain
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678759/
https://www.ncbi.nlm.nih.gov/pubmed/36595897
http://dx.doi.org/10.1016/j.xpro.2022.101836
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