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Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions

Phosphoserine (pSer) sites are primarily located within disordered protein regions, making it difficult to experimentally ascertain their effects on protein structure and function. Therefore, the production of (15)N- (and (13)C)-labeled proteins with site-specifically encoded pSer for NMR studies is...

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Detalles Bibliográficos
Autores principales:	Vesely, Cat Hoang, Reardon, Patrick N., Yu, Zhen, Barbar, Elisar, Mehl, Ryan A., Cooley, Richard B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2022
Materias:	Methods and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678770/ https://www.ncbi.nlm.nih.gov/pubmed/36265582 http://dx.doi.org/10.1016/j.jbc.2022.102613

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