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Optimized protocol for quantifying 5′ UTR-mediated translation initiation in S. cerevisiae using direct analysis of ribosome targeting

Direct analysis of ribosome targeting (DART) allows investigators to measure the translation initiation potential of thousands of RNAs in parallel. Here, we describe an optimized protocol for generating active translation extract from S. cerevisiae, followed by in vitro translation, purification of...

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Detalles Bibliográficos
Autores principales:	Lewis, Cole J.T., Niederer, Rachel O., Neupane, Ritam, Gilbert, Wendy V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678775/ https://www.ncbi.nlm.nih.gov/pubmed/36595943 http://dx.doi.org/10.1016/j.xpro.2022.101862

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