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Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials

The accumulation of protons in electro-active biofilms (EABfs) has been reported as a critical parameter determining produced currents at the anode since the very beginning of the studies on Bio-electrochemical systems (BESs). Even though the knowledge gained on the influence of this parameter on th...

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Autores principales: Pereira, João, Wang, Guanxiong, Sleutels, Tom, Hamelers, Bert, ter Heijne, Annemiek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678801/
https://www.ncbi.nlm.nih.gov/pubmed/36425753
http://dx.doi.org/10.1016/j.bioflm.2022.100092
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author Pereira, João
Wang, Guanxiong
Sleutels, Tom
Hamelers, Bert
ter Heijne, Annemiek
author_facet Pereira, João
Wang, Guanxiong
Sleutels, Tom
Hamelers, Bert
ter Heijne, Annemiek
author_sort Pereira, João
collection PubMed
description The accumulation of protons in electro-active biofilms (EABfs) has been reported as a critical parameter determining produced currents at the anode since the very beginning of the studies on Bio-electrochemical systems (BESs). Even though the knowledge gained on the influence of this parameter on the produced currents, its influence on EABfs growth is frequently overlooked. In this study, we quantified EABfs thicknesses in real-time and related them to the produced current at three buffer concentrations, two anode potentials and two acetate concentrations. The thickest EABfs (80 μm) and higher produced currents (2.5 A.m(−2)) were measured when a 50 mM buffer concentration was used. By combining the measured EABfs thicknesses with the pH in the anolyte, a simple model was developed to identify buffer limitations. Buffer limited EABfs with thicknesses of 15 and 42 μm were identified at −0.3 V vs Ag/AgCl when 10 and 50 mM buffer concentrations were used, respectively. At −0.2 V vs Ag/AgCl, the thicknesses of buffer limited EABfs decreased to 13 and 20 μm, respectively. The model also estimated buffer and acetate diffusion rates in EABfs and allowed to determine the boundary between a buffer and acetate limited EABfs. The diffusion rates reported in this study and the definition of the boundary between buffer and acetate limited EABfs provide a powerful tool to avoid limitations, leading to higher produced currents at the anode.
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spelling pubmed-96788012022-11-23 Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials Pereira, João Wang, Guanxiong Sleutels, Tom Hamelers, Bert ter Heijne, Annemiek Biofilm Article The accumulation of protons in electro-active biofilms (EABfs) has been reported as a critical parameter determining produced currents at the anode since the very beginning of the studies on Bio-electrochemical systems (BESs). Even though the knowledge gained on the influence of this parameter on the produced currents, its influence on EABfs growth is frequently overlooked. In this study, we quantified EABfs thicknesses in real-time and related them to the produced current at three buffer concentrations, two anode potentials and two acetate concentrations. The thickest EABfs (80 μm) and higher produced currents (2.5 A.m(−2)) were measured when a 50 mM buffer concentration was used. By combining the measured EABfs thicknesses with the pH in the anolyte, a simple model was developed to identify buffer limitations. Buffer limited EABfs with thicknesses of 15 and 42 μm were identified at −0.3 V vs Ag/AgCl when 10 and 50 mM buffer concentrations were used, respectively. At −0.2 V vs Ag/AgCl, the thicknesses of buffer limited EABfs decreased to 13 and 20 μm, respectively. The model also estimated buffer and acetate diffusion rates in EABfs and allowed to determine the boundary between a buffer and acetate limited EABfs. The diffusion rates reported in this study and the definition of the boundary between buffer and acetate limited EABfs provide a powerful tool to avoid limitations, leading to higher produced currents at the anode. Elsevier 2022-11-11 /pmc/articles/PMC9678801/ /pubmed/36425753 http://dx.doi.org/10.1016/j.bioflm.2022.100092 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pereira, João
Wang, Guanxiong
Sleutels, Tom
Hamelers, Bert
ter Heijne, Annemiek
Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials
title Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials
title_full Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials
title_fullStr Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials
title_full_unstemmed Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials
title_short Maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials
title_sort maximum thickness of non-buffer limited electro-active biofilms decreases at higher anode potentials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678801/
https://www.ncbi.nlm.nih.gov/pubmed/36425753
http://dx.doi.org/10.1016/j.bioflm.2022.100092
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