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Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment

The relationship between reactive oxygen and nitrogen species (ROS-RNS) secretion and the concomitant biocorrosion of degradable magnesium (Mg) materials is poorly understood. We found that Mg foils implanted short term in vivo (24 h) displayed large amounts of proinflammatory F4/80+/iNOS + macropha...

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Autores principales: Kwesiga, Maria P., Gillette, Amani A., Razaviamri, Fatemeh, Plank, Margaret E., Canull, Alexia L., Alesch, Zachary, He, Weilue, Lee, Bruce P., Guillory, Roger J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678810/
https://www.ncbi.nlm.nih.gov/pubmed/36439083
http://dx.doi.org/10.1016/j.bioactmat.2022.10.017
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author Kwesiga, Maria P.
Gillette, Amani A.
Razaviamri, Fatemeh
Plank, Margaret E.
Canull, Alexia L.
Alesch, Zachary
He, Weilue
Lee, Bruce P.
Guillory, Roger J.
author_facet Kwesiga, Maria P.
Gillette, Amani A.
Razaviamri, Fatemeh
Plank, Margaret E.
Canull, Alexia L.
Alesch, Zachary
He, Weilue
Lee, Bruce P.
Guillory, Roger J.
author_sort Kwesiga, Maria P.
collection PubMed
description The relationship between reactive oxygen and nitrogen species (ROS-RNS) secretion and the concomitant biocorrosion of degradable magnesium (Mg) materials is poorly understood. We found that Mg foils implanted short term in vivo (24 h) displayed large amounts of proinflammatory F4/80+/iNOS + macrophages at the interface. We sought to investigate the interplay between biodegrading Mg materials (98.6% Mg, AZ31 & AZ61) and macrophages (RAW 264.7) stimulated with lipopolysaccharide (RAW 264.7(LPS)) to induce ROS-RNS secretion. To test how these proinflammatory ROS-RNS secreting cells interact with Mg corrosion in vitro, Mg and AZ61 discs were suspended approximately 2 mm above a monolayer of RAW 264.7 cells, either with or without LPS. The surfaces of both materials showed acute (24 h) changes when incubated in the proinflammatory RAW 264.7(LPS) environment. Mg discs incubated with RAW 264.7(LPS) macrophages showed greater corrosion pitting, while AZ61 showed morphological and elemental bulk product changes via scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX). X-ray photoelectron spectroscopy (XPS) analysis showed a reduction in the Ca/P ratio of the surface products for AZ61 disc incubated with RAW 264.7(LPS), but not the Mg discs. Moreover, RAW 264.7(LPS) macrophages were found to be more viable in the acute biodegradative environment generated by Mg materials, as demonstrated by calcein-AM and cleaved (active) caspase-3 staining (CC3). LPS stimulation caused an increase in ROS-RNS, and a decrease in antioxidant peroxidase activity. Mg and AZ61 were found to change this ROS-RNS balance, independently of physiological antioxidant mechanisms. The findings highlight the complexity of the cellular driven acute inflammatory responses to different biodegradable Mg, and how it can potentially affect performance of these materials.
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spelling pubmed-96788102022-11-25 Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment Kwesiga, Maria P. Gillette, Amani A. Razaviamri, Fatemeh Plank, Margaret E. Canull, Alexia L. Alesch, Zachary He, Weilue Lee, Bruce P. Guillory, Roger J. Bioact Mater Article The relationship between reactive oxygen and nitrogen species (ROS-RNS) secretion and the concomitant biocorrosion of degradable magnesium (Mg) materials is poorly understood. We found that Mg foils implanted short term in vivo (24 h) displayed large amounts of proinflammatory F4/80+/iNOS + macrophages at the interface. We sought to investigate the interplay between biodegrading Mg materials (98.6% Mg, AZ31 & AZ61) and macrophages (RAW 264.7) stimulated with lipopolysaccharide (RAW 264.7(LPS)) to induce ROS-RNS secretion. To test how these proinflammatory ROS-RNS secreting cells interact with Mg corrosion in vitro, Mg and AZ61 discs were suspended approximately 2 mm above a monolayer of RAW 264.7 cells, either with or without LPS. The surfaces of both materials showed acute (24 h) changes when incubated in the proinflammatory RAW 264.7(LPS) environment. Mg discs incubated with RAW 264.7(LPS) macrophages showed greater corrosion pitting, while AZ61 showed morphological and elemental bulk product changes via scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX). X-ray photoelectron spectroscopy (XPS) analysis showed a reduction in the Ca/P ratio of the surface products for AZ61 disc incubated with RAW 264.7(LPS), but not the Mg discs. Moreover, RAW 264.7(LPS) macrophages were found to be more viable in the acute biodegradative environment generated by Mg materials, as demonstrated by calcein-AM and cleaved (active) caspase-3 staining (CC3). LPS stimulation caused an increase in ROS-RNS, and a decrease in antioxidant peroxidase activity. Mg and AZ61 were found to change this ROS-RNS balance, independently of physiological antioxidant mechanisms. The findings highlight the complexity of the cellular driven acute inflammatory responses to different biodegradable Mg, and how it can potentially affect performance of these materials. KeAi Publishing 2022-11-17 /pmc/articles/PMC9678810/ /pubmed/36439083 http://dx.doi.org/10.1016/j.bioactmat.2022.10.017 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Kwesiga, Maria P.
Gillette, Amani A.
Razaviamri, Fatemeh
Plank, Margaret E.
Canull, Alexia L.
Alesch, Zachary
He, Weilue
Lee, Bruce P.
Guillory, Roger J.
Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment
title Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment
title_full Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment
title_fullStr Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment
title_full_unstemmed Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment
title_short Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment
title_sort biodegradable magnesium materials regulate ros-rns balance in pro-inflammatory macrophage environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678810/
https://www.ncbi.nlm.nih.gov/pubmed/36439083
http://dx.doi.org/10.1016/j.bioactmat.2022.10.017
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