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Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration
Muscle regeneration requires the coordination of muscle stem cells, mesenchymal fibro-adipogenic progenitors (FAPs), and macrophages. How macrophages regulate the paracrine secretion of FAPs during the recovery process remains elusive. Herein, we systemically investigated the communication between C...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678897/ https://www.ncbi.nlm.nih.gov/pubmed/36411280 http://dx.doi.org/10.1038/s41467-022-34191-y |
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author | Nawaz, Allah Bilal, Muhammad Fujisaka, Shiho Kado, Tomonobu Aslam, Muhammad Rahil Ahmed, Saeed Okabe, Keisuke Igarashi, Yoshiko Watanabe, Yoshiyuki Kuwano, Takahide Tsuneyama, Koichi Nishimura, Ayumi Nishida, Yasuhiro Yamamoto, Seiji Sasahara, Masakiyo Imura, Johji Mori, Hisashi Matzuk, Martin M. Kudo, Fujimi Manabe, Ichiro Uezumi, Akiyoshi Nakagawa, Takashi Oishi, Yumiko Tobe, Kazuyuki |
author_facet | Nawaz, Allah Bilal, Muhammad Fujisaka, Shiho Kado, Tomonobu Aslam, Muhammad Rahil Ahmed, Saeed Okabe, Keisuke Igarashi, Yoshiko Watanabe, Yoshiyuki Kuwano, Takahide Tsuneyama, Koichi Nishimura, Ayumi Nishida, Yasuhiro Yamamoto, Seiji Sasahara, Masakiyo Imura, Johji Mori, Hisashi Matzuk, Martin M. Kudo, Fujimi Manabe, Ichiro Uezumi, Akiyoshi Nakagawa, Takashi Oishi, Yumiko Tobe, Kazuyuki |
author_sort | Nawaz, Allah |
collection | PubMed |
description | Muscle regeneration requires the coordination of muscle stem cells, mesenchymal fibro-adipogenic progenitors (FAPs), and macrophages. How macrophages regulate the paracrine secretion of FAPs during the recovery process remains elusive. Herein, we systemically investigated the communication between CD206(+) M2-like macrophages and FAPs during the recovery process using a transgenic mouse model. Depletion of CD206(+) M2-like macrophages or deletion of CD206(+) M2-like macrophages-specific TGF-β1 gene induces myogenesis and muscle regeneration. We show that depletion of CD206(+) M2-like macrophages activates FAPs and activated FAPs secrete follistatin, a promyogenic factor, thereby boosting the recovery process. Conversely, deletion of the FAP-specific follistatin gene results in impaired muscle stem cell function, enhanced fibrosis, and delayed muscle regeneration. Mechanistically, CD206(+) M2-like macrophages inhibit the secretion of FAP-derived follistatin via TGF-β signaling. Here we show that CD206(+) M2-like macrophages constitute a microenvironment for FAPs and may regulate the myogenic potential of muscle stem/satellite cells. |
format | Online Article Text |
id | pubmed-9678897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96788972022-11-23 Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration Nawaz, Allah Bilal, Muhammad Fujisaka, Shiho Kado, Tomonobu Aslam, Muhammad Rahil Ahmed, Saeed Okabe, Keisuke Igarashi, Yoshiko Watanabe, Yoshiyuki Kuwano, Takahide Tsuneyama, Koichi Nishimura, Ayumi Nishida, Yasuhiro Yamamoto, Seiji Sasahara, Masakiyo Imura, Johji Mori, Hisashi Matzuk, Martin M. Kudo, Fujimi Manabe, Ichiro Uezumi, Akiyoshi Nakagawa, Takashi Oishi, Yumiko Tobe, Kazuyuki Nat Commun Article Muscle regeneration requires the coordination of muscle stem cells, mesenchymal fibro-adipogenic progenitors (FAPs), and macrophages. How macrophages regulate the paracrine secretion of FAPs during the recovery process remains elusive. Herein, we systemically investigated the communication between CD206(+) M2-like macrophages and FAPs during the recovery process using a transgenic mouse model. Depletion of CD206(+) M2-like macrophages or deletion of CD206(+) M2-like macrophages-specific TGF-β1 gene induces myogenesis and muscle regeneration. We show that depletion of CD206(+) M2-like macrophages activates FAPs and activated FAPs secrete follistatin, a promyogenic factor, thereby boosting the recovery process. Conversely, deletion of the FAP-specific follistatin gene results in impaired muscle stem cell function, enhanced fibrosis, and delayed muscle regeneration. Mechanistically, CD206(+) M2-like macrophages inhibit the secretion of FAP-derived follistatin via TGF-β signaling. Here we show that CD206(+) M2-like macrophages constitute a microenvironment for FAPs and may regulate the myogenic potential of muscle stem/satellite cells. Nature Publishing Group UK 2022-11-21 /pmc/articles/PMC9678897/ /pubmed/36411280 http://dx.doi.org/10.1038/s41467-022-34191-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nawaz, Allah Bilal, Muhammad Fujisaka, Shiho Kado, Tomonobu Aslam, Muhammad Rahil Ahmed, Saeed Okabe, Keisuke Igarashi, Yoshiko Watanabe, Yoshiyuki Kuwano, Takahide Tsuneyama, Koichi Nishimura, Ayumi Nishida, Yasuhiro Yamamoto, Seiji Sasahara, Masakiyo Imura, Johji Mori, Hisashi Matzuk, Martin M. Kudo, Fujimi Manabe, Ichiro Uezumi, Akiyoshi Nakagawa, Takashi Oishi, Yumiko Tobe, Kazuyuki Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration |
title | Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration |
title_full | Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration |
title_fullStr | Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration |
title_full_unstemmed | Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration |
title_short | Depletion of CD206(+) M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration |
title_sort | depletion of cd206(+) m2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678897/ https://www.ncbi.nlm.nih.gov/pubmed/36411280 http://dx.doi.org/10.1038/s41467-022-34191-y |
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