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Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues

The transcriptional repressor REV-ERB-α, encoded by Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), has been considered to play an essential role in the skeletal muscle oxidative capacity adaptation and muscle mass control. Also, this molecule regulates autophagy via the repression of autopha...

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Autores principales: da Rocha, Alisson L., Pinto, Ana P., Bedo, Bruno L. S., Morais, Gustavo P., Oliveira, Luciana C., Carolino, Ruither O. G., Pauli, Jose R., Simabuco, Fernando M., de Moura, Leandro P., Ropelle, Eduardo R., Cintra, Dennys E., Rivas, Donato A., da Silva, Adelino S. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678899/
https://www.ncbi.nlm.nih.gov/pubmed/36411310
http://dx.doi.org/10.1038/s41598-022-24277-4
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author da Rocha, Alisson L.
Pinto, Ana P.
Bedo, Bruno L. S.
Morais, Gustavo P.
Oliveira, Luciana C.
Carolino, Ruither O. G.
Pauli, Jose R.
Simabuco, Fernando M.
de Moura, Leandro P.
Ropelle, Eduardo R.
Cintra, Dennys E.
Rivas, Donato A.
da Silva, Adelino S. R.
author_facet da Rocha, Alisson L.
Pinto, Ana P.
Bedo, Bruno L. S.
Morais, Gustavo P.
Oliveira, Luciana C.
Carolino, Ruither O. G.
Pauli, Jose R.
Simabuco, Fernando M.
de Moura, Leandro P.
Ropelle, Eduardo R.
Cintra, Dennys E.
Rivas, Donato A.
da Silva, Adelino S. R.
author_sort da Rocha, Alisson L.
collection PubMed
description The transcriptional repressor REV-ERB-α, encoded by Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), has been considered to play an essential role in the skeletal muscle oxidative capacity adaptation and muscle mass control. Also, this molecule regulates autophagy via the repression of autophagy-related genes both in skeletal muscle and brain regions. Classically, training programs based on endurance or strength characteristics enhance skeletal muscle mass content and/or oxidative capacity, leading to autophagy activation in several tissues. Thus, it seems that REV-ERB-α regulates similar responses induced by exercise. However, how this molecule responds to different exercise models/intensities in different tissues is still unclear. Therefore, the main aim was to characterize the responses of REV-ERB-α and autophagy-related genes to different exercise protocols (endurance/interval run/strength) in distinct tissues (gastrocnemius, soleus and hippocampus). Since REV-ERB-α presents a circadian rhythm, the analyses were performed in a time-course manner. The endurance and strength groups attenuated REV-ERB-α transcriptional response during the time course in gastrocnemius and soleus. Conversely, the interval group enhanced the Nr1d1 expression in the hippocampus. All protocols downregulated the REV-ERB-α protein levels in gastrocnemius following the exercise session with concomitant nuclear exclusion. The major autophagy-related genes presented downregulation after the exercise session in all analyzed tissues. Altogether, these results highlight that REV-ERB-α is extremely sensitive to physical exercise stimuli, including different models and intensities in skeletal muscle and the hippocampus.
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spelling pubmed-96788992022-11-23 Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues da Rocha, Alisson L. Pinto, Ana P. Bedo, Bruno L. S. Morais, Gustavo P. Oliveira, Luciana C. Carolino, Ruither O. G. Pauli, Jose R. Simabuco, Fernando M. de Moura, Leandro P. Ropelle, Eduardo R. Cintra, Dennys E. Rivas, Donato A. da Silva, Adelino S. R. Sci Rep Article The transcriptional repressor REV-ERB-α, encoded by Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), has been considered to play an essential role in the skeletal muscle oxidative capacity adaptation and muscle mass control. Also, this molecule regulates autophagy via the repression of autophagy-related genes both in skeletal muscle and brain regions. Classically, training programs based on endurance or strength characteristics enhance skeletal muscle mass content and/or oxidative capacity, leading to autophagy activation in several tissues. Thus, it seems that REV-ERB-α regulates similar responses induced by exercise. However, how this molecule responds to different exercise models/intensities in different tissues is still unclear. Therefore, the main aim was to characterize the responses of REV-ERB-α and autophagy-related genes to different exercise protocols (endurance/interval run/strength) in distinct tissues (gastrocnemius, soleus and hippocampus). Since REV-ERB-α presents a circadian rhythm, the analyses were performed in a time-course manner. The endurance and strength groups attenuated REV-ERB-α transcriptional response during the time course in gastrocnemius and soleus. Conversely, the interval group enhanced the Nr1d1 expression in the hippocampus. All protocols downregulated the REV-ERB-α protein levels in gastrocnemius following the exercise session with concomitant nuclear exclusion. The major autophagy-related genes presented downregulation after the exercise session in all analyzed tissues. Altogether, these results highlight that REV-ERB-α is extremely sensitive to physical exercise stimuli, including different models and intensities in skeletal muscle and the hippocampus. Nature Publishing Group UK 2022-11-21 /pmc/articles/PMC9678899/ /pubmed/36411310 http://dx.doi.org/10.1038/s41598-022-24277-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
da Rocha, Alisson L.
Pinto, Ana P.
Bedo, Bruno L. S.
Morais, Gustavo P.
Oliveira, Luciana C.
Carolino, Ruither O. G.
Pauli, Jose R.
Simabuco, Fernando M.
de Moura, Leandro P.
Ropelle, Eduardo R.
Cintra, Dennys E.
Rivas, Donato A.
da Silva, Adelino S. R.
Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues
title Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues
title_full Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues
title_fullStr Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues
title_full_unstemmed Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues
title_short Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues
title_sort exercise alters the circadian rhythm of rev-erb-α and downregulates autophagy-related genes in peripheral and central tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678899/
https://www.ncbi.nlm.nih.gov/pubmed/36411310
http://dx.doi.org/10.1038/s41598-022-24277-4
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