Cargando…

Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis

Mycobacterium bovis (M. bovis) is the zoonotic bacterium responsible for bovine tuberculosis. An attenuated form of M. bovis, Bacillus Calmette-Guerin (BCG), is a modified live vaccine known to provide variable protection in cattle and other species. Protection for this vaccine is defined as a reduc...

Descripción completa

Detalles Bibliográficos
Autores principales: Kanipe, C., Boggiatto, P. M., Putz, E. J., Palmer, M. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678917/
https://www.ncbi.nlm.nih.gov/pubmed/36425039
http://dx.doi.org/10.3389/fmicb.2022.1048648
_version_ 1784834095424995328
author Kanipe, C.
Boggiatto, P. M.
Putz, E. J.
Palmer, M. V.
author_facet Kanipe, C.
Boggiatto, P. M.
Putz, E. J.
Palmer, M. V.
author_sort Kanipe, C.
collection PubMed
description Mycobacterium bovis (M. bovis) is the zoonotic bacterium responsible for bovine tuberculosis. An attenuated form of M. bovis, Bacillus Calmette-Guerin (BCG), is a modified live vaccine known to provide variable protection in cattle and other species. Protection for this vaccine is defined as a reduction in disease severity rather than prevention of infection and is determined by evaluation of the characteristic lesion of tuberculosis: the granuloma. Despite its recognized ability to decrease disease severity, the mechanism by which BCG imparts protection remains poorly understood. Understanding the histopathologic differences between granulomas which form in BCG vaccinates compared to non-vaccinates may help identify how BCG imparts protection and lead to an improved vaccine. Utilizing special stains and image analysis software, we examined 88 lymph nodes obtained from BGC-vaccinated and non-vaccinated animals experimentally infected with M. bovis. We evaluated the number of granulomas, their size, severity (grade), density of multinucleated giant cells (MNGC), and the amounts of necrosis, mineralization, and fibrosis. BCG vaccinates had fewer granulomas overall and smaller high-grade granulomas with less necrosis than non-vaccinates. The relative numbers of high- and low- grade lesions were similar as were the amounts of mineralization and the density of MNGC. The amount of fibrosis was higher in low-grade granulomas from vaccinates compared to non-vaccinates. Collectively, these findings suggest that BCG vaccination reduces bacterial establishment, resulting in the formation of fewer granulomas. In granulomas that form, BCG has a protective effect by containing their size, reducing the relative amount of necrosis, and increasing fibrosis in low-grade lesions. Vaccination did not affect the amount of mineralization or density of MNGC.
format Online
Article
Text
id pubmed-9678917
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96789172022-11-23 Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis Kanipe, C. Boggiatto, P. M. Putz, E. J. Palmer, M. V. Front Microbiol Microbiology Mycobacterium bovis (M. bovis) is the zoonotic bacterium responsible for bovine tuberculosis. An attenuated form of M. bovis, Bacillus Calmette-Guerin (BCG), is a modified live vaccine known to provide variable protection in cattle and other species. Protection for this vaccine is defined as a reduction in disease severity rather than prevention of infection and is determined by evaluation of the characteristic lesion of tuberculosis: the granuloma. Despite its recognized ability to decrease disease severity, the mechanism by which BCG imparts protection remains poorly understood. Understanding the histopathologic differences between granulomas which form in BCG vaccinates compared to non-vaccinates may help identify how BCG imparts protection and lead to an improved vaccine. Utilizing special stains and image analysis software, we examined 88 lymph nodes obtained from BGC-vaccinated and non-vaccinated animals experimentally infected with M. bovis. We evaluated the number of granulomas, their size, severity (grade), density of multinucleated giant cells (MNGC), and the amounts of necrosis, mineralization, and fibrosis. BCG vaccinates had fewer granulomas overall and smaller high-grade granulomas with less necrosis than non-vaccinates. The relative numbers of high- and low- grade lesions were similar as were the amounts of mineralization and the density of MNGC. The amount of fibrosis was higher in low-grade granulomas from vaccinates compared to non-vaccinates. Collectively, these findings suggest that BCG vaccination reduces bacterial establishment, resulting in the formation of fewer granulomas. In granulomas that form, BCG has a protective effect by containing their size, reducing the relative amount of necrosis, and increasing fibrosis in low-grade lesions. Vaccination did not affect the amount of mineralization or density of MNGC. Frontiers Media S.A. 2022-11-08 /pmc/articles/PMC9678917/ /pubmed/36425039 http://dx.doi.org/10.3389/fmicb.2022.1048648 Text en Copyright © 2022 Kanipe, Boggiatto, Putz and Palmer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kanipe, C.
Boggiatto, P. M.
Putz, E. J.
Palmer, M. V.
Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis
title Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis
title_full Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis
title_fullStr Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis
title_full_unstemmed Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis
title_short Histopathologic differences in granulomas of Mycobacterium bovis bacille Calmette Guérin (BCG) vaccinated and non-vaccinated cattle with bovine tuberculosis
title_sort histopathologic differences in granulomas of mycobacterium bovis bacille calmette guérin (bcg) vaccinated and non-vaccinated cattle with bovine tuberculosis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678917/
https://www.ncbi.nlm.nih.gov/pubmed/36425039
http://dx.doi.org/10.3389/fmicb.2022.1048648
work_keys_str_mv AT kanipec histopathologicdifferencesingranulomasofmycobacteriumbovisbacillecalmetteguerinbcgvaccinatedandnonvaccinatedcattlewithbovinetuberculosis
AT boggiattopm histopathologicdifferencesingranulomasofmycobacteriumbovisbacillecalmetteguerinbcgvaccinatedandnonvaccinatedcattlewithbovinetuberculosis
AT putzej histopathologicdifferencesingranulomasofmycobacteriumbovisbacillecalmetteguerinbcgvaccinatedandnonvaccinatedcattlewithbovinetuberculosis
AT palmermv histopathologicdifferencesingranulomasofmycobacteriumbovisbacillecalmetteguerinbcgvaccinatedandnonvaccinatedcattlewithbovinetuberculosis