Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides

In this study, we investigated the structural features of the polysaccharide obtained from Craterellus cornucopioides (CCP2) by high-performance liquid chromatography, Fourier transform infrared spectroscopy and ion chromatography. The results showed that CCP2 was a catenarian pyranose that principa...

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Autores principales: Zhang, Caixuan, Shu, Ying, Li, Yang, Guo, Mingzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678937/
https://www.ncbi.nlm.nih.gov/pubmed/36424922
http://dx.doi.org/10.3389/fnut.2022.1017431
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author Zhang, Caixuan
Shu, Ying
Li, Yang
Guo, Mingzhu
author_facet Zhang, Caixuan
Shu, Ying
Li, Yang
Guo, Mingzhu
author_sort Zhang, Caixuan
collection PubMed
description In this study, we investigated the structural features of the polysaccharide obtained from Craterellus cornucopioides (CCP2) by high-performance liquid chromatography, Fourier transform infrared spectroscopy and ion chromatography. The results showed that CCP2 was a catenarian pyranose that principally comprised of mannose, galactose, glucose, and xylose in the ratio of 1.86: 1.57: 1.00: 1.14, with a molecular weight of 8.28 × 10(4) Da. Moreover, the immunoregulation effect of CCP2 was evaluated both in vitro and in vivo. It displayed a remarkable immunological activity and activation in RAW264.7 cells by enhancing the phagocytosis of macrophages in a dose-dependent manner without showing cytotoxicity at the concentrations of 10–200 μg/mL in vitro. Additionally, Histopathological analysis indicated the protective function of CCP2 against immunosuppression induced by cyclophosphamide (Cy). Meanwhile, the intake of CCP2 had better immunoregulatory activity for immunosuppression BALB/c mice model. After prevention by CCP2, the spleen and thymus weight indexes of BALB/c mice model were significantly increased. The RT-qPCR and Western Blot results provided comprehensive evidence that the CCP2 could activate macrophages by enhancing the production of cytokines (IL-2, IL-6, and IL-8) and upregulating the protein expression of cell membrane receptor TLR4 and its downstream protein kinase (TRAF6, TRIF, and NF-κB p65) production of immunosuppressive mice through TLR4-NFκB p65 pathway. The results demonstrated that CCP2 could be a potential prebiotic and might provide meaningful information for further research on the immune mechanism.
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spelling pubmed-96789372022-11-23 Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides Zhang, Caixuan Shu, Ying Li, Yang Guo, Mingzhu Front Nutr Nutrition In this study, we investigated the structural features of the polysaccharide obtained from Craterellus cornucopioides (CCP2) by high-performance liquid chromatography, Fourier transform infrared spectroscopy and ion chromatography. The results showed that CCP2 was a catenarian pyranose that principally comprised of mannose, galactose, glucose, and xylose in the ratio of 1.86: 1.57: 1.00: 1.14, with a molecular weight of 8.28 × 10(4) Da. Moreover, the immunoregulation effect of CCP2 was evaluated both in vitro and in vivo. It displayed a remarkable immunological activity and activation in RAW264.7 cells by enhancing the phagocytosis of macrophages in a dose-dependent manner without showing cytotoxicity at the concentrations of 10–200 μg/mL in vitro. Additionally, Histopathological analysis indicated the protective function of CCP2 against immunosuppression induced by cyclophosphamide (Cy). Meanwhile, the intake of CCP2 had better immunoregulatory activity for immunosuppression BALB/c mice model. After prevention by CCP2, the spleen and thymus weight indexes of BALB/c mice model were significantly increased. The RT-qPCR and Western Blot results provided comprehensive evidence that the CCP2 could activate macrophages by enhancing the production of cytokines (IL-2, IL-6, and IL-8) and upregulating the protein expression of cell membrane receptor TLR4 and its downstream protein kinase (TRAF6, TRIF, and NF-κB p65) production of immunosuppressive mice through TLR4-NFκB p65 pathway. The results demonstrated that CCP2 could be a potential prebiotic and might provide meaningful information for further research on the immune mechanism. Frontiers Media S.A. 2022-11-08 /pmc/articles/PMC9678937/ /pubmed/36424922 http://dx.doi.org/10.3389/fnut.2022.1017431 Text en Copyright © 2022 Zhang, Shu, Li and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Zhang, Caixuan
Shu, Ying
Li, Yang
Guo, Mingzhu
Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides
title Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides
title_full Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides
title_fullStr Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides
title_full_unstemmed Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides
title_short Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides
title_sort extraction and immunomodulatory activity of the polysaccharide obtained from craterellus cornucopioides
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678937/
https://www.ncbi.nlm.nih.gov/pubmed/36424922
http://dx.doi.org/10.3389/fnut.2022.1017431
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