Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides
In this study, we investigated the structural features of the polysaccharide obtained from Craterellus cornucopioides (CCP2) by high-performance liquid chromatography, Fourier transform infrared spectroscopy and ion chromatography. The results showed that CCP2 was a catenarian pyranose that principa...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678937/ https://www.ncbi.nlm.nih.gov/pubmed/36424922 http://dx.doi.org/10.3389/fnut.2022.1017431 |
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author | Zhang, Caixuan Shu, Ying Li, Yang Guo, Mingzhu |
author_facet | Zhang, Caixuan Shu, Ying Li, Yang Guo, Mingzhu |
author_sort | Zhang, Caixuan |
collection | PubMed |
description | In this study, we investigated the structural features of the polysaccharide obtained from Craterellus cornucopioides (CCP2) by high-performance liquid chromatography, Fourier transform infrared spectroscopy and ion chromatography. The results showed that CCP2 was a catenarian pyranose that principally comprised of mannose, galactose, glucose, and xylose in the ratio of 1.86: 1.57: 1.00: 1.14, with a molecular weight of 8.28 × 10(4) Da. Moreover, the immunoregulation effect of CCP2 was evaluated both in vitro and in vivo. It displayed a remarkable immunological activity and activation in RAW264.7 cells by enhancing the phagocytosis of macrophages in a dose-dependent manner without showing cytotoxicity at the concentrations of 10–200 μg/mL in vitro. Additionally, Histopathological analysis indicated the protective function of CCP2 against immunosuppression induced by cyclophosphamide (Cy). Meanwhile, the intake of CCP2 had better immunoregulatory activity for immunosuppression BALB/c mice model. After prevention by CCP2, the spleen and thymus weight indexes of BALB/c mice model were significantly increased. The RT-qPCR and Western Blot results provided comprehensive evidence that the CCP2 could activate macrophages by enhancing the production of cytokines (IL-2, IL-6, and IL-8) and upregulating the protein expression of cell membrane receptor TLR4 and its downstream protein kinase (TRAF6, TRIF, and NF-κB p65) production of immunosuppressive mice through TLR4-NFκB p65 pathway. The results demonstrated that CCP2 could be a potential prebiotic and might provide meaningful information for further research on the immune mechanism. |
format | Online Article Text |
id | pubmed-9678937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96789372022-11-23 Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides Zhang, Caixuan Shu, Ying Li, Yang Guo, Mingzhu Front Nutr Nutrition In this study, we investigated the structural features of the polysaccharide obtained from Craterellus cornucopioides (CCP2) by high-performance liquid chromatography, Fourier transform infrared spectroscopy and ion chromatography. The results showed that CCP2 was a catenarian pyranose that principally comprised of mannose, galactose, glucose, and xylose in the ratio of 1.86: 1.57: 1.00: 1.14, with a molecular weight of 8.28 × 10(4) Da. Moreover, the immunoregulation effect of CCP2 was evaluated both in vitro and in vivo. It displayed a remarkable immunological activity and activation in RAW264.7 cells by enhancing the phagocytosis of macrophages in a dose-dependent manner without showing cytotoxicity at the concentrations of 10–200 μg/mL in vitro. Additionally, Histopathological analysis indicated the protective function of CCP2 against immunosuppression induced by cyclophosphamide (Cy). Meanwhile, the intake of CCP2 had better immunoregulatory activity for immunosuppression BALB/c mice model. After prevention by CCP2, the spleen and thymus weight indexes of BALB/c mice model were significantly increased. The RT-qPCR and Western Blot results provided comprehensive evidence that the CCP2 could activate macrophages by enhancing the production of cytokines (IL-2, IL-6, and IL-8) and upregulating the protein expression of cell membrane receptor TLR4 and its downstream protein kinase (TRAF6, TRIF, and NF-κB p65) production of immunosuppressive mice through TLR4-NFκB p65 pathway. The results demonstrated that CCP2 could be a potential prebiotic and might provide meaningful information for further research on the immune mechanism. Frontiers Media S.A. 2022-11-08 /pmc/articles/PMC9678937/ /pubmed/36424922 http://dx.doi.org/10.3389/fnut.2022.1017431 Text en Copyright © 2022 Zhang, Shu, Li and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Zhang, Caixuan Shu, Ying Li, Yang Guo, Mingzhu Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides |
title | Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides |
title_full | Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides |
title_fullStr | Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides |
title_full_unstemmed | Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides |
title_short | Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides |
title_sort | extraction and immunomodulatory activity of the polysaccharide obtained from craterellus cornucopioides |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678937/ https://www.ncbi.nlm.nih.gov/pubmed/36424922 http://dx.doi.org/10.3389/fnut.2022.1017431 |
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