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SARS-CoV-2 infection induces activation of ferroptosis in human placenta

Ferroptosis, a regulated non-apoptotic form of cell death, has been implicated in the response to varied types of infectious agents including virus. In this study, we sought to determine whether SARS-CoV-2 infection can induce activation of ferroptosis in the human placenta. We collected placentas f...

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Autores principales: Wang, Bingbing, Shen, Wei-Bin, Yang, Peixin, Turan, Sifa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679405/
https://www.ncbi.nlm.nih.gov/pubmed/36425527
http://dx.doi.org/10.3389/fcell.2022.1022747
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author Wang, Bingbing
Shen, Wei-Bin
Yang, Peixin
Turan, Sifa
author_facet Wang, Bingbing
Shen, Wei-Bin
Yang, Peixin
Turan, Sifa
author_sort Wang, Bingbing
collection PubMed
description Ferroptosis, a regulated non-apoptotic form of cell death, has been implicated in the response to varied types of infectious agents including virus. In this study, we sought to determine whether SARS-CoV-2 infection can induce activation of ferroptosis in the human placenta. We collected placentas from 23 pregnant females with laboratory-confirmed SARS-CoV-2 following delivery and then used RNA in situ hybridization assay for detection of viral positive-sense strand (PSS) to confirm that these placentas have been infected. We also used immunohistochemistry assay to assess expression levels of acyl-CoA synthetase long-chain family member 4 (ACSL4), an essential executioner of ferroptosis in the same specimens. Our results showed that ACSL4 expression was significantly increased in the group with positive positive-sense strand staining compared to their negative counterparts (p = 0.00022). Furthermore, we found that there was a positive trend for increased PSS staining along with increased ACSL4 expression. Our study supports that ferroptosis is activated in the response to SARS-CoV-2 infection in the human placenta, highlighting a molecular mechanism potentially linking this coronavirus infection and pathogenesis of adverse pregnancy outcomes.
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spelling pubmed-96794052022-11-23 SARS-CoV-2 infection induces activation of ferroptosis in human placenta Wang, Bingbing Shen, Wei-Bin Yang, Peixin Turan, Sifa Front Cell Dev Biol Cell and Developmental Biology Ferroptosis, a regulated non-apoptotic form of cell death, has been implicated in the response to varied types of infectious agents including virus. In this study, we sought to determine whether SARS-CoV-2 infection can induce activation of ferroptosis in the human placenta. We collected placentas from 23 pregnant females with laboratory-confirmed SARS-CoV-2 following delivery and then used RNA in situ hybridization assay for detection of viral positive-sense strand (PSS) to confirm that these placentas have been infected. We also used immunohistochemistry assay to assess expression levels of acyl-CoA synthetase long-chain family member 4 (ACSL4), an essential executioner of ferroptosis in the same specimens. Our results showed that ACSL4 expression was significantly increased in the group with positive positive-sense strand staining compared to their negative counterparts (p = 0.00022). Furthermore, we found that there was a positive trend for increased PSS staining along with increased ACSL4 expression. Our study supports that ferroptosis is activated in the response to SARS-CoV-2 infection in the human placenta, highlighting a molecular mechanism potentially linking this coronavirus infection and pathogenesis of adverse pregnancy outcomes. Frontiers Media S.A. 2022-11-08 /pmc/articles/PMC9679405/ /pubmed/36425527 http://dx.doi.org/10.3389/fcell.2022.1022747 Text en Copyright © 2022 Wang, Shen, Yang and Turan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wang, Bingbing
Shen, Wei-Bin
Yang, Peixin
Turan, Sifa
SARS-CoV-2 infection induces activation of ferroptosis in human placenta
title SARS-CoV-2 infection induces activation of ferroptosis in human placenta
title_full SARS-CoV-2 infection induces activation of ferroptosis in human placenta
title_fullStr SARS-CoV-2 infection induces activation of ferroptosis in human placenta
title_full_unstemmed SARS-CoV-2 infection induces activation of ferroptosis in human placenta
title_short SARS-CoV-2 infection induces activation of ferroptosis in human placenta
title_sort sars-cov-2 infection induces activation of ferroptosis in human placenta
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679405/
https://www.ncbi.nlm.nih.gov/pubmed/36425527
http://dx.doi.org/10.3389/fcell.2022.1022747
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