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Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation

Microorganisms naturally present at environmental contaminated sites are capable of biodegrading, biotransforming, or removing contaminants in soil and groundwater through bioremediation processes. Cleanup strategies and goals for site remediation can be effectively achieved by bioremediation levera...

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Autores principales: Key, Trent A., Sorsby, Skyler J., Wang, Yingnan, Madison, Andrew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679620/
https://www.ncbi.nlm.nih.gov/pubmed/36425033
http://dx.doi.org/10.3389/fmicb.2022.958742
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author Key, Trent A.
Sorsby, Skyler J.
Wang, Yingnan
Madison, Andrew S.
author_facet Key, Trent A.
Sorsby, Skyler J.
Wang, Yingnan
Madison, Andrew S.
author_sort Key, Trent A.
collection PubMed
description Microorganisms naturally present at environmental contaminated sites are capable of biodegrading, biotransforming, or removing contaminants in soil and groundwater through bioremediation processes. Cleanup strategies and goals for site remediation can be effectively achieved by bioremediation leveraging the capabilities of microorganisms to biotransform contaminants into lesser or non-toxic end products; however, reproducible success can be limited by inadequate design or performance monitoring. A group of biological analyses collectively termed molecular biological tools (MBTs) can be used to assess the contaminant-degrading capabilities and activities of microorganisms present in the environment and appropriately implement bioremediation approaches. While successful bioremediation has been demonstrated through previously described lab-scale studies and field-scale implementation for a variety of environmental contaminants, design and performance monitoring of bioremediation has often been limited to inferring biodegradation potential, occurrence, and pathways based on site geochemistry or lab-scale studies. Potential field-scale application of MBTs presents the opportunity to more precisely design and monitor site-specific bioremediation approaches. To promote standardization and successful implementation of bioremediation, a framework for field-scale application of MBTs within a multiple lines of evidence (MLOE) approach is presented. The framework consists of three stages: (i) “Assessment” to evaluate naturally occurring biogeochemical conditions and screen for potential applicability of bioremediation, (ii) “Design” to define a site-specific bioremediation approach and inform amendment selection, and (iii) “Performance Monitoring” to generate data to measure or infer bioremediation progress following implementation. This framework is introduced to synthesize the complexities of environmental microbiology and guide field-scale application of MBTs to assess bioremediation potential and inform site decision-making.
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spelling pubmed-96796202022-11-23 Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation Key, Trent A. Sorsby, Skyler J. Wang, Yingnan Madison, Andrew S. Front Microbiol Microbiology Microorganisms naturally present at environmental contaminated sites are capable of biodegrading, biotransforming, or removing contaminants in soil and groundwater through bioremediation processes. Cleanup strategies and goals for site remediation can be effectively achieved by bioremediation leveraging the capabilities of microorganisms to biotransform contaminants into lesser or non-toxic end products; however, reproducible success can be limited by inadequate design or performance monitoring. A group of biological analyses collectively termed molecular biological tools (MBTs) can be used to assess the contaminant-degrading capabilities and activities of microorganisms present in the environment and appropriately implement bioremediation approaches. While successful bioremediation has been demonstrated through previously described lab-scale studies and field-scale implementation for a variety of environmental contaminants, design and performance monitoring of bioremediation has often been limited to inferring biodegradation potential, occurrence, and pathways based on site geochemistry or lab-scale studies. Potential field-scale application of MBTs presents the opportunity to more precisely design and monitor site-specific bioremediation approaches. To promote standardization and successful implementation of bioremediation, a framework for field-scale application of MBTs within a multiple lines of evidence (MLOE) approach is presented. The framework consists of three stages: (i) “Assessment” to evaluate naturally occurring biogeochemical conditions and screen for potential applicability of bioremediation, (ii) “Design” to define a site-specific bioremediation approach and inform amendment selection, and (iii) “Performance Monitoring” to generate data to measure or infer bioremediation progress following implementation. This framework is introduced to synthesize the complexities of environmental microbiology and guide field-scale application of MBTs to assess bioremediation potential and inform site decision-making. Frontiers Media S.A. 2022-11-08 /pmc/articles/PMC9679620/ /pubmed/36425033 http://dx.doi.org/10.3389/fmicb.2022.958742 Text en Copyright © 2022 Key, Sorsby, Wang and Madison. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Key, Trent A.
Sorsby, Skyler J.
Wang, Yingnan
Madison, Andrew S.
Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation
title Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation
title_full Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation
title_fullStr Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation
title_full_unstemmed Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation
title_short Framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation
title_sort framework for field-scale application of molecular biological tools to support natural and enhanced bioremediation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679620/
https://www.ncbi.nlm.nih.gov/pubmed/36425033
http://dx.doi.org/10.3389/fmicb.2022.958742
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