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Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679739/ https://www.ncbi.nlm.nih.gov/pubmed/36425980 http://dx.doi.org/10.1016/j.dib.2022.108744 |
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author | Rahmeh, Rita Akbar, Abrar Alomirah, Husam Kishk, Mohamed Al-Ateeqi, Abdulaziz Al-Milhm, Salah Shajan, Anisha Akbar, Batool Al-Merri, Shafeah Alotaibi, Mohammad Esposito, Alfonso |
author_facet | Rahmeh, Rita Akbar, Abrar Alomirah, Husam Kishk, Mohamed Al-Ateeqi, Abdulaziz Al-Milhm, Salah Shajan, Anisha Akbar, Batool Al-Merri, Shafeah Alotaibi, Mohammad Esposito, Alfonso |
author_sort | Rahmeh, Rita |
collection | PubMed |
description | Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three samples, and libraries were prepared using custom fusion primers of the 16S rRNA gene and sequenced on Illumina HiSeq 2500 platform. 16S rRNA gene sequences were aligned against the SILVA database SSU release 138. The high-throughput sequencing data are available at the NCBI database under the Bioproject PRJNA814013. This work describes camel milk's bacterial diversity among different geographical locations and seasons. The distribution of alpha diversity measures among camel milk sample groups collected from different geographical locations and seasons is presented. A significant effect of these parameters on camel milk's bacterial diversity was shown. Linear discriminant analysis (LefSe) showed significant differentially abundant bacteria at the phylum, class, order, family and genus level among the three locations and seasons. LefSe identified a total of 83 and 40 differentially abundant genera in the different geographical locations and seasons, respectively. More details about the bacterial composition of raw camel milk at the phylum and genus level can be found in research article [1]. These data can be used to compare the diversity of milk bacterial community between different milk producing species and camels from different parts of the world. Besides, these findings will contribute to our understanding of the camel microbiome structure and might be useful for designing an appropriate control program in the camel dairy herd. The data described in this article are available in Mendeley Data [2]. |
format | Online Article Text |
id | pubmed-9679739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96797392022-11-23 Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing Rahmeh, Rita Akbar, Abrar Alomirah, Husam Kishk, Mohamed Al-Ateeqi, Abdulaziz Al-Milhm, Salah Shajan, Anisha Akbar, Batool Al-Merri, Shafeah Alotaibi, Mohammad Esposito, Alfonso Data Brief Data Article Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three samples, and libraries were prepared using custom fusion primers of the 16S rRNA gene and sequenced on Illumina HiSeq 2500 platform. 16S rRNA gene sequences were aligned against the SILVA database SSU release 138. The high-throughput sequencing data are available at the NCBI database under the Bioproject PRJNA814013. This work describes camel milk's bacterial diversity among different geographical locations and seasons. The distribution of alpha diversity measures among camel milk sample groups collected from different geographical locations and seasons is presented. A significant effect of these parameters on camel milk's bacterial diversity was shown. Linear discriminant analysis (LefSe) showed significant differentially abundant bacteria at the phylum, class, order, family and genus level among the three locations and seasons. LefSe identified a total of 83 and 40 differentially abundant genera in the different geographical locations and seasons, respectively. More details about the bacterial composition of raw camel milk at the phylum and genus level can be found in research article [1]. These data can be used to compare the diversity of milk bacterial community between different milk producing species and camels from different parts of the world. Besides, these findings will contribute to our understanding of the camel microbiome structure and might be useful for designing an appropriate control program in the camel dairy herd. The data described in this article are available in Mendeley Data [2]. Elsevier 2022-11-11 /pmc/articles/PMC9679739/ /pubmed/36425980 http://dx.doi.org/10.1016/j.dib.2022.108744 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Rahmeh, Rita Akbar, Abrar Alomirah, Husam Kishk, Mohamed Al-Ateeqi, Abdulaziz Al-Milhm, Salah Shajan, Anisha Akbar, Batool Al-Merri, Shafeah Alotaibi, Mohammad Esposito, Alfonso Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
title | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
title_full | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
title_fullStr | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
title_full_unstemmed | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
title_short | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
title_sort | data on microbial diversity of camel milk microbiota determined by 16s rrna gene sequencing |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679739/ https://www.ncbi.nlm.nih.gov/pubmed/36425980 http://dx.doi.org/10.1016/j.dib.2022.108744 |
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