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Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing

Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three...

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Autores principales: Rahmeh, Rita, Akbar, Abrar, Alomirah, Husam, Kishk, Mohamed, Al-Ateeqi, Abdulaziz, Al-Milhm, Salah, Shajan, Anisha, Akbar, Batool, Al-Merri, Shafeah, Alotaibi, Mohammad, Esposito, Alfonso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679739/
https://www.ncbi.nlm.nih.gov/pubmed/36425980
http://dx.doi.org/10.1016/j.dib.2022.108744
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author Rahmeh, Rita
Akbar, Abrar
Alomirah, Husam
Kishk, Mohamed
Al-Ateeqi, Abdulaziz
Al-Milhm, Salah
Shajan, Anisha
Akbar, Batool
Al-Merri, Shafeah
Alotaibi, Mohammad
Esposito, Alfonso
author_facet Rahmeh, Rita
Akbar, Abrar
Alomirah, Husam
Kishk, Mohamed
Al-Ateeqi, Abdulaziz
Al-Milhm, Salah
Shajan, Anisha
Akbar, Batool
Al-Merri, Shafeah
Alotaibi, Mohammad
Esposito, Alfonso
author_sort Rahmeh, Rita
collection PubMed
description Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three samples, and libraries were prepared using custom fusion primers of the 16S rRNA gene and sequenced on Illumina HiSeq 2500 platform. 16S rRNA gene sequences were aligned against the SILVA database SSU release 138. The high-throughput sequencing data are available at the NCBI database under the Bioproject PRJNA814013. This work describes camel milk's bacterial diversity among different geographical locations and seasons. The distribution of alpha diversity measures among camel milk sample groups collected from different geographical locations and seasons is presented. A significant effect of these parameters on camel milk's bacterial diversity was shown. Linear discriminant analysis (LefSe) showed significant differentially abundant bacteria at the phylum, class, order, family and genus level among the three locations and seasons. LefSe identified a total of 83 and 40 differentially abundant genera in the different geographical locations and seasons, respectively. More details about the bacterial composition of raw camel milk at the phylum and genus level can be found in research article [1]. These data can be used to compare the diversity of milk bacterial community between different milk producing species and camels from different parts of the world. Besides, these findings will contribute to our understanding of the camel microbiome structure and might be useful for designing an appropriate control program in the camel dairy herd. The data described in this article are available in Mendeley Data [2].
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spelling pubmed-96797392022-11-23 Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing Rahmeh, Rita Akbar, Abrar Alomirah, Husam Kishk, Mohamed Al-Ateeqi, Abdulaziz Al-Milhm, Salah Shajan, Anisha Akbar, Batool Al-Merri, Shafeah Alotaibi, Mohammad Esposito, Alfonso Data Brief Data Article Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three samples, and libraries were prepared using custom fusion primers of the 16S rRNA gene and sequenced on Illumina HiSeq 2500 platform. 16S rRNA gene sequences were aligned against the SILVA database SSU release 138. The high-throughput sequencing data are available at the NCBI database under the Bioproject PRJNA814013. This work describes camel milk's bacterial diversity among different geographical locations and seasons. The distribution of alpha diversity measures among camel milk sample groups collected from different geographical locations and seasons is presented. A significant effect of these parameters on camel milk's bacterial diversity was shown. Linear discriminant analysis (LefSe) showed significant differentially abundant bacteria at the phylum, class, order, family and genus level among the three locations and seasons. LefSe identified a total of 83 and 40 differentially abundant genera in the different geographical locations and seasons, respectively. More details about the bacterial composition of raw camel milk at the phylum and genus level can be found in research article [1]. These data can be used to compare the diversity of milk bacterial community between different milk producing species and camels from different parts of the world. Besides, these findings will contribute to our understanding of the camel microbiome structure and might be useful for designing an appropriate control program in the camel dairy herd. The data described in this article are available in Mendeley Data [2]. Elsevier 2022-11-11 /pmc/articles/PMC9679739/ /pubmed/36425980 http://dx.doi.org/10.1016/j.dib.2022.108744 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Rahmeh, Rita
Akbar, Abrar
Alomirah, Husam
Kishk, Mohamed
Al-Ateeqi, Abdulaziz
Al-Milhm, Salah
Shajan, Anisha
Akbar, Batool
Al-Merri, Shafeah
Alotaibi, Mohammad
Esposito, Alfonso
Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
title Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
title_full Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
title_fullStr Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
title_full_unstemmed Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
title_short Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
title_sort data on microbial diversity of camel milk microbiota determined by 16s rrna gene sequencing
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679739/
https://www.ncbi.nlm.nih.gov/pubmed/36425980
http://dx.doi.org/10.1016/j.dib.2022.108744
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