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Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection

CD40 and CD154 are one of the best-characterized costimulatory molecules essential for adaptive immunity, which extensively involved in T and B cell activation, IgM Ab production, isotype class switching, germinal center formation and affinity maturation. However, the functionality of CD40 and CD154...

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Autores principales: Su, Ning, Hu, Chong-bin, Shao, Tong, Jin, Chun-yu, Li, Hao, Ji, Jian-fei, Qin, Lu-lu, Fan, Dong-Dong, Lin, Ai-fu, Xiang, Li-xin, Shao, Jian-zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680072/
https://www.ncbi.nlm.nih.gov/pubmed/36420488
http://dx.doi.org/10.1016/j.fsirep.2021.100038
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author Su, Ning
Hu, Chong-bin
Shao, Tong
Jin, Chun-yu
Li, Hao
Ji, Jian-fei
Qin, Lu-lu
Fan, Dong-Dong
Lin, Ai-fu
Xiang, Li-xin
Shao, Jian-zhong
author_facet Su, Ning
Hu, Chong-bin
Shao, Tong
Jin, Chun-yu
Li, Hao
Ji, Jian-fei
Qin, Lu-lu
Fan, Dong-Dong
Lin, Ai-fu
Xiang, Li-xin
Shao, Jian-zhong
author_sort Su, Ning
collection PubMed
description CD40 and CD154 are one of the best-characterized costimulatory molecules essential for adaptive immunity, which extensively involved in T and B cell activation, IgM Ab production, isotype class switching, germinal center formation and affinity maturation. However, the functionality of CD40 and CD154 in IgZ-mediated immunity remains limited. In this study, we explored the regulatory role of Cd40-Cd154 interaction in IgZ-mediated antibacterial immunity in zebrafish. The results showed that the IgZ-mediated antibacterial response can be significantly induced in response to A. hydrophila infection. The percentage of Cd40(+)IgZ(+) B cells and the production of IgZ Ab were substantially increased upon A. hydrophila stimulation, but these reactions were markedly declined in Cd154 blockade fish by administering anti-Cd154 Ab or recombinant sCd40-Ig protein, accompanied with the impairment of the vaccine-initiated IgZ-mediated immunoprotection of fish against A. hydrophila infection. These observations suggested the essential role of Cd40-Cd154 interaction in IgZ-mediated bacterial immunity. Notably, the Cd40 and Cd154 costimulatory signals are required for a TD antigen-induced IgZ immunity, but are not indispensable for a TI antigen-induced IgZ immune response. These findings indicated the differential role of Cd40-Cd154 interaction in bacterial TD and TI antigen-induced IgZ immunity, which suggested the existence of diverse regulatory mechanisms underlying IgZ-mediated antibacterial immune reactions. To our knowledge, this is the first report to show the functional role of Cd40-Cd154 costimulatory signaling pathway in IgZ-mediated immune defense against bacterial infection. We hope this study will improve the current understanding of the coevolution between the IgZ/IgT immunoglobins and CD40/CD154 costimulatory molecules.
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spelling pubmed-96800722022-11-22 Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection Su, Ning Hu, Chong-bin Shao, Tong Jin, Chun-yu Li, Hao Ji, Jian-fei Qin, Lu-lu Fan, Dong-Dong Lin, Ai-fu Xiang, Li-xin Shao, Jian-zhong Fish Shellfish Immunol Rep Article CD40 and CD154 are one of the best-characterized costimulatory molecules essential for adaptive immunity, which extensively involved in T and B cell activation, IgM Ab production, isotype class switching, germinal center formation and affinity maturation. However, the functionality of CD40 and CD154 in IgZ-mediated immunity remains limited. In this study, we explored the regulatory role of Cd40-Cd154 interaction in IgZ-mediated antibacterial immunity in zebrafish. The results showed that the IgZ-mediated antibacterial response can be significantly induced in response to A. hydrophila infection. The percentage of Cd40(+)IgZ(+) B cells and the production of IgZ Ab were substantially increased upon A. hydrophila stimulation, but these reactions were markedly declined in Cd154 blockade fish by administering anti-Cd154 Ab or recombinant sCd40-Ig protein, accompanied with the impairment of the vaccine-initiated IgZ-mediated immunoprotection of fish against A. hydrophila infection. These observations suggested the essential role of Cd40-Cd154 interaction in IgZ-mediated bacterial immunity. Notably, the Cd40 and Cd154 costimulatory signals are required for a TD antigen-induced IgZ immunity, but are not indispensable for a TI antigen-induced IgZ immune response. These findings indicated the differential role of Cd40-Cd154 interaction in bacterial TD and TI antigen-induced IgZ immunity, which suggested the existence of diverse regulatory mechanisms underlying IgZ-mediated antibacterial immune reactions. To our knowledge, this is the first report to show the functional role of Cd40-Cd154 costimulatory signaling pathway in IgZ-mediated immune defense against bacterial infection. We hope this study will improve the current understanding of the coevolution between the IgZ/IgT immunoglobins and CD40/CD154 costimulatory molecules. Elsevier 2021-11-14 /pmc/articles/PMC9680072/ /pubmed/36420488 http://dx.doi.org/10.1016/j.fsirep.2021.100038 Text en © 2021 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Su, Ning
Hu, Chong-bin
Shao, Tong
Jin, Chun-yu
Li, Hao
Ji, Jian-fei
Qin, Lu-lu
Fan, Dong-Dong
Lin, Ai-fu
Xiang, Li-xin
Shao, Jian-zhong
Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
title Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
title_full Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
title_fullStr Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
title_full_unstemmed Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
title_short Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
title_sort functional role of cd40 and cd154 costimulatory signals in igz-mediated immunity against bacterial infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680072/
https://www.ncbi.nlm.nih.gov/pubmed/36420488
http://dx.doi.org/10.1016/j.fsirep.2021.100038
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