Cargando…
Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens
The study was conducted to evaluate the vaccination effects on rohu, Labeo rohita head kidney tissues while assessing the vaccine efficacy of Aeromonas hydrophila antigens. Six acclimatized rohu groups were immunized with three antigenic formulations (outer membrane proteins, somatic and whole-cell...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680105/ https://www.ncbi.nlm.nih.gov/pubmed/36420497 http://dx.doi.org/10.1016/j.fsirep.2021.100025 |
_version_ | 1784834337060945920 |
---|---|
author | Biswas, Avijit Dash, Gadadhar Mali, Prasenjit Joardar, Siddhartha Narayan Dey, Biswadeep Roy, Anwesha Karmakar, Sutanu |
author_facet | Biswas, Avijit Dash, Gadadhar Mali, Prasenjit Joardar, Siddhartha Narayan Dey, Biswadeep Roy, Anwesha Karmakar, Sutanu |
author_sort | Biswas, Avijit |
collection | PubMed |
description | The study was conducted to evaluate the vaccination effects on rohu, Labeo rohita head kidney tissues while assessing the vaccine efficacy of Aeromonas hydrophila antigens. Six acclimatized rohu groups were immunized with three antigenic formulations (outer membrane proteins, somatic and whole-cell antigen) @ 200 µg/fish and also with equal volume of Freund's incomplete adjuvant (FIA), separately. Simultaneously, two non-vaccinated groups, i.e., injected with FIA (100µl), normal saline solutions (0.85%) and one control without injection were maintained for 28 days. All rohu were challenged with median lethal dose of A. hydrophila (2.85 × 10(6) cells/rohu) intraperitoneally. After 7 days, highest cumulative mortality (%) of ˃88% was found for all non-vaccinated groups. During histopathological observations in head kidney tissues of all treatment and control groups, numerous histopathological changes in the nephritic cells like mild loss of typical tubular epithelial lining, necrosis, thickening of renal epithelial lining, haemorrhages, inflammation, distorted and widening of the lumen with vacuolated surrounding and the constricted lumen of nephritic tubules were noticed for vaccinated rohu in contrast to non vaccinated groups before A. hydrophila challenge. In case of all non-vaccinated fish, including control, extensive degenerated and necrotized head kidney tissues were observed, whereas it was least observed in vaccinated rohu after 7 days A. hydrophila challenge. Results suggest that OMPs antigen along with FIA was the premier vaccine approach for improving resistance to Aeromonas disease and reduce mortality in rohu. Similarly, vaccination with all three antigenic formulations, preferably when applied along with FIA, can effectively protect the head kidney against A. hydrophila infection. |
format | Online Article Text |
id | pubmed-9680105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96801052022-11-22 Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens Biswas, Avijit Dash, Gadadhar Mali, Prasenjit Joardar, Siddhartha Narayan Dey, Biswadeep Roy, Anwesha Karmakar, Sutanu Fish Shellfish Immunol Rep Article The study was conducted to evaluate the vaccination effects on rohu, Labeo rohita head kidney tissues while assessing the vaccine efficacy of Aeromonas hydrophila antigens. Six acclimatized rohu groups were immunized with three antigenic formulations (outer membrane proteins, somatic and whole-cell antigen) @ 200 µg/fish and also with equal volume of Freund's incomplete adjuvant (FIA), separately. Simultaneously, two non-vaccinated groups, i.e., injected with FIA (100µl), normal saline solutions (0.85%) and one control without injection were maintained for 28 days. All rohu were challenged with median lethal dose of A. hydrophila (2.85 × 10(6) cells/rohu) intraperitoneally. After 7 days, highest cumulative mortality (%) of ˃88% was found for all non-vaccinated groups. During histopathological observations in head kidney tissues of all treatment and control groups, numerous histopathological changes in the nephritic cells like mild loss of typical tubular epithelial lining, necrosis, thickening of renal epithelial lining, haemorrhages, inflammation, distorted and widening of the lumen with vacuolated surrounding and the constricted lumen of nephritic tubules were noticed for vaccinated rohu in contrast to non vaccinated groups before A. hydrophila challenge. In case of all non-vaccinated fish, including control, extensive degenerated and necrotized head kidney tissues were observed, whereas it was least observed in vaccinated rohu after 7 days A. hydrophila challenge. Results suggest that OMPs antigen along with FIA was the premier vaccine approach for improving resistance to Aeromonas disease and reduce mortality in rohu. Similarly, vaccination with all three antigenic formulations, preferably when applied along with FIA, can effectively protect the head kidney against A. hydrophila infection. Elsevier 2021-09-05 /pmc/articles/PMC9680105/ /pubmed/36420497 http://dx.doi.org/10.1016/j.fsirep.2021.100025 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Biswas, Avijit Dash, Gadadhar Mali, Prasenjit Joardar, Siddhartha Narayan Dey, Biswadeep Roy, Anwesha Karmakar, Sutanu Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens |
title | Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens |
title_full | Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens |
title_fullStr | Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens |
title_full_unstemmed | Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens |
title_short | Histopathology of head kidney tissues in challenged rohu, Labeo rohita Hamilton after vaccinating with Aeromonas hydrophila antigens |
title_sort | histopathology of head kidney tissues in challenged rohu, labeo rohita hamilton after vaccinating with aeromonas hydrophila antigens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680105/ https://www.ncbi.nlm.nih.gov/pubmed/36420497 http://dx.doi.org/10.1016/j.fsirep.2021.100025 |
work_keys_str_mv | AT biswasavijit histopathologyofheadkidneytissuesinchallengedrohulabeorohitahamiltonaftervaccinatingwithaeromonashydrophilaantigens AT dashgadadhar histopathologyofheadkidneytissuesinchallengedrohulabeorohitahamiltonaftervaccinatingwithaeromonashydrophilaantigens AT maliprasenjit histopathologyofheadkidneytissuesinchallengedrohulabeorohitahamiltonaftervaccinatingwithaeromonashydrophilaantigens AT joardarsiddharthanarayan histopathologyofheadkidneytissuesinchallengedrohulabeorohitahamiltonaftervaccinatingwithaeromonashydrophilaantigens AT deybiswadeep histopathologyofheadkidneytissuesinchallengedrohulabeorohitahamiltonaftervaccinatingwithaeromonashydrophilaantigens AT royanwesha histopathologyofheadkidneytissuesinchallengedrohulabeorohitahamiltonaftervaccinatingwithaeromonashydrophilaantigens AT karmakarsutanu histopathologyofheadkidneytissuesinchallengedrohulabeorohitahamiltonaftervaccinatingwithaeromonashydrophilaantigens |