An optimized NGS sample preparation protocol for in vitro CRISPR screens

This standardized protocol describes the preparation of PCR amplified and purified samples from human cell lines passaged and collected from CRISPR screening. High-quality samples can be used to perform next-generation sequencing (NGS) to uncover changes in sgRNA abundance from the timepoint at whic...

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Detalles Bibliográficos
Autores principales: Wohlhieter, Corrin A., Uddin, Fathema, Quintanal-Villalonga, Àlvaro, Poirier, John T., Sen, Triparna, Rudin, Charles M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680194/
http://dx.doi.org/10.1016/j.xpro.2021.100390

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9680194/
http://dx.doi.org/10.1016/j.xpro.2021.100390

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